Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Rearing and Morphological Characters for Sex Determination
2.2. Sequence Analysis
2.3. RNA Extraction and cDNA Preparation
2.4. RT-PCR and RT-qPCR
2.5. Preparation of Double Stranded RNA (dsRNA) with In Vitro Transcription
2.6. Injection or Feeding Treatment of dsRNA to Young Larvae
2.7. Preparation of dsRNA with Recombinant Bacteria
2.8. Quantification of dsRNA Produced from Recombinant Bacteria
2.9. Pretreatment of Recombinant Bacteria Using Heat and Sonication
2.10. Feeding Assay of Recombinant Bacteria Expressing dsRNA
2.11. Statistical Analysis
3. Results
3.1. Morphological Characters of Female and Male Z. scutellata Adults
3.2. Prediction of Transformer-2 (Zs-Tra2) of Z. Scutellata and Expression Profile
3.3. Male-Biased Sex Ratio after RNAi of Zs-Tra2 Expression
3.4. Construction of Recombinant E. coli Expressing dsRNA Specific to Zs-Tra2 and Its Effect on Sex Transformation
3.5. Effect of Feeding Recombinant E. coli on Sex Ratio of Z. scutellata
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Al Baki, M.A.; Vatanparast, M.; Kim, Y. Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2. Insects 2020, 11, 211. https://doi.org/10.3390/insects11040211
Al Baki MA, Vatanparast M, Kim Y. Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2. Insects. 2020; 11(4):211. https://doi.org/10.3390/insects11040211
Chicago/Turabian StyleAl Baki, Md. Abdullah, Mohammad Vatanparast, and Yonggyun Kim. 2020. "Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2" Insects 11, no. 4: 211. https://doi.org/10.3390/insects11040211