Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Plasmids
2.2. Cell Transfection
2.3. Cell Imaging
2.4. Western Blotting
3. Results
3.1. Strategy for a Novel Genetic Code Expansion System to Control Gene Expression in B. mori Cell Lines
3.2. Testing of the Novel Genetic Code Expansion System in BmE Cell Line
3.3. Application of the Novel Genetic Code Expansion System in BmNs Cell Line
3.4. Efficient and Orthogonal Bombyx mori Genetic Code Extension System
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Primer Name | Sequence (5′ to 3′) |
---|---|---|
DsRed | DsRed-F (Xba I+Spe I) | GCTCTAGAGGGACTAGTATGGTGCGCTCCTCCAAG |
DsRed-R (Sph I) | ACATGCATGCCTACAGGAACAGGTGGTGG | |
EGFP | EGFP-F (Sph I) | ACATGCATGCATGGTGAGCAAGGGCGAG |
EGFP-R (Hind III+BsrG I) | CCCAAGCTTTTACTTGTACAGCTCGTCCATG |
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Lu, W.; Wang, R.; Wang, P.; Ma, S.; Xia, Q. Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines. Insects 2021, 12, 1081. https://doi.org/10.3390/insects12121081
Lu W, Wang R, Wang P, Ma S, Xia Q. Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines. Insects. 2021; 12(12):1081. https://doi.org/10.3390/insects12121081
Chicago/Turabian StyleLu, Wei, Ruolin Wang, Pan Wang, Sanyuan Ma, and Qingyou Xia. 2021. "Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines" Insects 12, no. 12: 1081. https://doi.org/10.3390/insects12121081