The Draft Genome of Yellow Stem Borer, an Agriculturally Important Pest, Provides Molecular Insights into Its Biology, Development and Specificity Towards Rice for Infestation
by
Divya Kattupalli
1,†, 
Kalyani M. Barbadikar
1,†,
Vishalakshi Balija
1,
Suneel Ballichatla
1,
Athulya R
2,
Ayyagari Phani Padmakumari
2,
Swati Saxena
3,
Kishor Gaikwad
3,
Sridhar Yerram
2,
Premalatha Kokku
4 and
Maganti Sheshu Madhav
1,* 1
Biotechnology Section, Division of Crop Improvement, ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
2
Entomology Section, Division of Crop Protection, ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
3
Genomics Lab, ICAR-National Institute of Plant Biotechnology, New Delhi 110012, India
4
Department of Chemistry, Osmania University, Hyderabad 500007, India
*
Author to whom correspondence should be addressed.
†
Authors contributed equally as the first author.
Insects 2021, 12(6), 563; https://doi.org/10.3390/insects12060563
Submission received: 24 April 2021
/
Revised: 20 May 2021
/
Accepted: 23 May 2021
/
Published: 19 June 2021
Simple Summary
Yellow stem borer (YSB), is the most destructive and widely occurring pest that attacks rice throughout the growing season. Rice (Oryza sativa L.) is a major staple cereal worldwide, providing essential caloric requirements for more than half of the world’s population. Annual losses to rice borers are approximately 5–10%, but losses in individual fields may reach up to 50–60%. The use of traditional pest management strategies in controlling YSB is somewhat challenging due to its unique internal feeding habit. Genome sequence information of economically important crop pests is important for designing or developing pest-resistant rice varieties. In an approach to achieve this, we present our first-ever study on the draft genome sequence of YSB. The information provided from our current study might be useful in developing genome-based approaches for the management of pest species.
Abstract
Yellow stem borer (YSB), Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae), a major monophagous insect pest of rice, causes significant yield losses. The rice–YSB interaction is very dynamic, making it difficult for management. The development of resistant lines has been unsuccessful as there are no effective resistant sources in the germplasm. Genome information is necessary for a better understanding of interaction with rice in terms of its recognition, response, and infestation mechanism. The draft genome of YSB is predicted to have 46,057 genes with an estimated size of 308 Mb, being correlated with the flow cytometry analysis. The existence of complex metabolic mechanisms and genes related to specific behavior was identified, being conditioned by a higher level of regulation. We deciphered the possible visual, olfactory, and gustatory mechanisms responsible for its evolution as a monophagous pest. Comparative genomic analysis revealed that YSB is unique in the way it has evolved. The obvious presence of high-immunity-related genes, well-developed RNAi machinery, and diverse effectors provides a means for developing genomic tools for its management. The identified 21,696 SSR markers can be utilized for diversity analysis of populations across the rice-growing regions. We present the first draft genome of YSB. The information emanated paves a way for biologists to design novel pest management strategies as well as for the industry to design new classes of safer and specific insecticide molecules.
Keywords:
YSB genome; Scirpophaga; monophagous; rice pest; effectors; venom-like proteins; insect pest; immune genes
