Identification and Tissue Expression Profiles of Odorant Receptor Genes in the Green Peach Aphid Myzus persicae
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Rearing and Sample Collection
2.2. RNA Extraction, cDNA Library Construction and Illumina Sequencing
2.3. Bioinformatics Analysis
2.4. Identification of M. persicae ORs by Bioinformatics
2.5. Phylogenetic Analysis
2.6. Verification of OR Sequences by Cloning and Sequencing
2.7. Genomic Structure Analysis of M. persicae ORs
2.8. Expression Profiles of M. persicae ORs in Different Tissues
3. Results
3.1. Identification of M. persicae ORs
3.2. Phylogenetic Analyses of MperORs
3.3. Genomic Structure of M. persicae ORs
3.4. Expression Profiles of M. perszicae ORs in Different Tissues
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | ORF (bp) | Amino Acids | Scaffold * | Antennae RPKM | Head RPKM | Legs RPKM | Body RPKM | Homology Search with A. Pisum | ||
---|---|---|---|---|---|---|---|---|---|---|
Most Similar | %Identity | TM Domains | ||||||||
MperORco | 1494 | 498 | 173 (307,518–314,584) | 16.04 | 1.32 | 0.15 | 1.05 | ApisOR1 | 96 | 7 |
MperOR2 | 1203 | 401 | 200 (329,352–332,846) | 4.19 | 0.14 | 0.07 | 0.14 | ApisOR2 | 93 | 5 |
MperOR3 | 1206 | 402 | 27 (538,815–544,733) | 2.76 | 0.14 | 0.04 | 0.08 | ApisOR3 | 94 | 8 |
MperOR4 | 1167 | 389 | 2 (1,669,537–1,680,404) | 0.66 | 0.25 | 0.08 | 0.12 | ApisOR4 | 94 | 6 |
MperOR5 | 1104 | 368 | 185 (390,783–396,720) | 1.28 | 0.08 | 0.00 | 0.02 | ApisOR5 | 87 | 8 |
MperOR9 | 1107 | 369 | 52 (795,957–799,660) | 1.23 | 0.03 | 0.03 | 0.03 | ApisOR9 | 82 | 6 |
MperOR10 | 1122 | 374 | 448 (232,245–234,867) | 1.23 | 0.03 | 0.03 | 0.03 | ApisOR10 | 68 | 7 |
MperOR17 | 1296 | 432 | 54 (655,522–659,088) | 1.36 | 4.15 | 1.44 | 5.60 | ApisOR17 | 84 | 6 |
MperOR20 | 1266 | 422 | 27 (295,168–299,537) | 3.45 | 0.14 | 0.62 | 0.32 | ApisOR20 | 89 | 6 |
MperOR21 | 1272 | 424 | 27 (273,498–275,759) | 1.39 | 0.20 | 0.05 | 0.05 | ApisOR21 | 84 | 7 |
MperOR22 | 1290 | 430 | 27 (286,505–290,732) | 0.09 | 0.00 | 0.00 | 0.00 | ApisOR22 | 80 | 6 |
MperOR23 | 1248 | 416 | 128 (606,788–609,435) | 0.42 | 0.00 | 0.00 | 0.02 | ApisOR23 | 90 | 7 |
MperOR24 | 1224 | 408 | 128 (619,838–621,712) | 1.49 | 5.02 | 1.90 | 4.38 | ApisOR23 | 43 | 4 |
MperOR25 | 1203 | 401 | 1011 (10,792–14,561) | 1.30 | 0.01 | 0.01 | 0.05 | ApisOR25 | 80 | 5 |
MperOR29 | 1242 | 414 | 495 (178,577–182,414) | 0.21 | 0.06 | 0.00 | 0.01 | ApisOR29 | 72 | 6 |
MperOR35 | 1221 | 407 | 139 (21,194–26,450) | 1.32 | 0.01 | 0.01 | 0.08 | ApisOR35 | 59 | 6 |
MperOR36 | 1221 | 407 | 0 (1,027,879–1,031,452) | 0.19 | 0.02 | 0.00 | 0.00 | ApisOR36 | 75 | 6 |
MperOR37 | 1251 | 417 | 533 (71,681–75,764) | 1.95 | 0.01 | 0.05 | 0.04 | ApisOR37 | 88 | 7 |
MperOR38 | 1221 | 407 | 16 (1,217,873–1,219,749) | 0.26 | 0.03 | 0.07 | 0.05 | ApisOR38 | 84 | 6 |
MperOR39 | 1188 | 396 | 257 (222,543–224,270) | 0.12 | 0.00 | 0.02 | 0.03 | ApisOR39 | 93 | 5 |
MperOR40 | 1266 | 422 | 268 (227,342–230,883) | 0.53 | 0.00 | 0.01 | 0.00 | ApisOR40 | 86 | 6 |
MperOR41 | 1248 | 416 | 268 (236,969–239,632) | 0.61 | 0.04 | 0.18 | 0.28 | ApisOR41 | 84 | 6 |
MperOR42 | 1290 | 430 | 129 (610,966–615,169) | 0.12 | 2.27 | 0.21 | 0.65 | ApisOR42 | 75 | 7 |
MperOR43 | 1254 | 418 | 138 (86,064–89,109) | 1.32 | 0.15 | 0.01 | 0.08 | ApisOR43 | 92 | 8 |
MperOR44 | 1140 | 380 | 115 (575,915–582,437) | 0.10 | 0.05 | 0.00 | 0.18 | ApisOR44 | 59 | 8 |
MperOR45 | 1296 | 432 | 13 (1,040,637–1,046,400) | 0.50 | 0.22 | 0.05 | 0.64 | ApisOR45 | 58 | 5 |
MperOR47 | 1302 | 434 | 109 (556,358–561,734) | 0.08 | 0.00 | 0.05 | 0.13 | ApisOR47 | 73 | 7 |
MperOR51 | 1293 | 431 | 1192 (10,158–17,868) | 0.53 | 0.06 | 0.00 | 0.03 | ApisOR70 | 70 | 6 |
MperOR53 | 1281 | 427 | 700 (104,988–111,553) | 0.84 | 0.04 | 0.00 | 0.02 | ApisOR53 | 70 | 6 |
MperOR64 | 1290 | 430 | 380 (259,518–265,853) | 0.53 | 0.06 | 0.00 | 0.03 | ApisOR64 | 71 | 5 |
MperOR67 | 1239 | 413 | 354 (145,937–151,752) | 5.87 | 0.06 | 0.05 | 0.09 | ApisOR67 | 81 | 6 |
MperOR69 | 1626 | 542 | 304 (223,678–231,005) | 0.36 | 0.04 | 0.06 | 0.02 | ApisOR69 | 59 | 5 |
MperOR78 | 1290 | 430 | 140 (453,957–457,387) | 0.36 | 0.05 | 0.03 | 0.08 | ApisOR78 | 62 | 6 |
Gene Name | Genomic DNA Size (bp) and Strand (+/−) | No. of Intron | Total Length of Introns (bp) | Average Intron Size (bp) | No. of Exon | Total Length of Exons (bp) | Average Exon Size (bp) |
---|---|---|---|---|---|---|---|
MperORco | 7066(+) | 8 | 5572 | 697 | 9 | 1494 | 166 |
MperOR2 | 3494(−) | 1 | 2291 | 2291 | 2 | 1203 | 602 |
MperOR3 | 5918(+) | 3 | 4712 | 1571 | 4 | 1206 | 302 |
MperOR4 | 10,867(+) | 5 | 9700 | 1940 | 6 | 1167 | 195 |
MperOR5 | 5937(+) | 4 | 4833 | 1208 | 5 | 1104 | 221 |
MperOR9 | 3703(+) | 4 | 2596 | 649 | 5 | 1107 | 221 |
MperOR10 | 2622(+) | 4 | 1500 | 375 | 5 | 1122 | 224 |
MperOR17 | 3566(−) | 5 | 2270 | 454 | 6 | 1296 | 216 |
MperOR20 | 4369(−) | 5 | 3103 | 621 | 6 | 1266 | 211 |
MperOR21 | 2261(−) | 5 | 989 | 198 | 6 | 1272 | 212 |
MperOR22 | 4227(−) | 5 | 2937 | 587 | 6 | 1290 | 215 |
MperOR23 | 2647(−) | 5 | 1399 | 280 | 6 | 1248 | 208 |
MperOR24 | 1874(−) | 4 | 650 | 163 | 5 | 1224 | 245 |
MperOR25 | 3769(+) | 5 | 2566 | 513 | 6 | 1203 | 201 |
MperOR29 | 3837(+) | 5 | 2595 | 519 | 6 | 1242 | 207 |
MperOR35 | 5256(−) | 5 | 4035 | 807 | 6 | 1221 | 204 |
MperOR36 | 3573(−) | 5 | 2352 | 470 | 6 | 1221 | 204 |
MperOR37 | 4083(+) | 5 | 2832 | 566 | 6 | 1251 | 209 |
MperOR38 | 1876(+) | 4 | 655 | 164 | 5 | 1221 | 244 |
MperOR39 | 1727(+) | 4 | 539 | 135 | 5 | 1188 | 238 |
MperOR40 | 3541(−) | 5 | 2275 | 455 | 6 | 1266 | 211 |
MperOR41 | 2663(+) | 5 | 1415 | 283 | 6 | 1248 | 208 |
MperOR42 | 4203(+) | 5 | 2913 | 583 | 6 | 1290 | 215 |
MperOR43 | 3045(+) | 5 | 1791 | 358 | 6 | 1254 | 209 |
MperOR44 | 6522(−) | 5 | 5382 | 1076 | 6 | 1140 | 190 |
MperOR45 | 5763(+) | 5 | 4467 | 893 | 6 | 1296 | 216 |
MperOR47 | 5376(+) | 5 | 4074 | 815 | 6 | 1302 | 217 |
MperOR51 | 7710(−) | 5 | 6417 | 1283 | 6 | 1293 | 216 |
MperOR53 | 6565(+) | 5 | 5284 | 1057 | 6 | 1281 | 214 |
MperOR64 | 6335(+) | 5 | 5045 | 1009 | 6 | 1290 | 215 |
MperOR67 | 5815(−) | 6 | 4576 | 763 | 7 | 1239 | 177 |
MperOR69 | 7327(+) | 5 | 5701 | 1140 | 6 | 1626 | 271 |
MperOR78 | 3430(+) | 5 | 2140 | 428 | 6 | 1290 | 215 |
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Liu, J.; Xie, J.; Khashaveh, A.; Zhou, J.; Zhang, Y.; Dong, H.; Cong, B.; Gu, S. Identification and Tissue Expression Profiles of Odorant Receptor Genes in the Green Peach Aphid Myzus persicae. Insects 2022, 13, 398. https://doi.org/10.3390/insects13050398
Liu J, Xie J, Khashaveh A, Zhou J, Zhang Y, Dong H, Cong B, Gu S. Identification and Tissue Expression Profiles of Odorant Receptor Genes in the Green Peach Aphid Myzus persicae. Insects. 2022; 13(5):398. https://doi.org/10.3390/insects13050398
Chicago/Turabian StyleLiu, Jingtao, Jiaoxin Xie, Adel Khashaveh, Jingjiang Zhou, Yongjun Zhang, Hui Dong, Bin Cong, and Shaohua Gu. 2022. "Identification and Tissue Expression Profiles of Odorant Receptor Genes in the Green Peach Aphid Myzus persicae" Insects 13, no. 5: 398. https://doi.org/10.3390/insects13050398