Effect of Different Host Plants on the Diversity of Gut Bacterial Communities of Spodoptera frugiperda (J. E. Smith, 1797)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Rearing and Sample Collection
2.2. DNA Extraction, Library Preparation, and High-Throughput Sequencing
2.3. Statistical and Bioinformatics Analysis
3. Results
3.1. General Profile of 16S rRNA Sequencing Data
3.2. Comparison of the Bacterial Communities
3.3. Functional Prediction of Microbiota
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Taxonomy | Group | ZM | TA | OS | LJF | LJL | DO | F-Value | p-Value |
---|---|---|---|---|---|---|---|---|---|
Phylum | Firmicutes | 98.39 ± 1.21 a | 80.80 ± 12.71 b | 83.93 ± 2.47 ab | 96.68 ± 4.68 a | 94.04 ± 0.90 ab | 98.97 ± 0.44 a | 5.731 | 0.006 |
Actinobacteriota | 0.37 ± 0.20 b | 13.33 ± 3.89 a | 11.90 ± 2.26 a | 0.12 ± 0.06 b | 0.51 ± 0.16 b | 0.25 ± 0.08 b | 35.936 | <0.001 | |
Proteobacteria | 0.79 ± 0.10 b | 0.40 ± 0.15 b | 0.75 ± 0.38 b | 0.47 ± 0.27 b | 5.17 ± 0.87 a | 0.69 ± 0.47 b | 51.616 | <0.001 | |
Cyanobacteria | 0.54 ± 0.31 a | 0.02 ± 0.01 b | 0.14 ± 0.21 ab | 0.02 ± 0.02 b | 0.03 ± 0.05 b | 0.01 ± 0.01 b | 5.565 | 0.007 | |
Bacteroidota | 0.04 ± 0.03 bc | 0.01 ± 0.01 c | 0.57 ± 0.13 a | 0.01 ± 0.01 bc | 0.19 ± 0.07 b | 0.07 ± 0.05 bc | 34.112 | <0.001 | |
Family | Enterococcaceae | 92.35 ± 5.94 a | 82.25 ± 6.20 ab | 68.50 ± 9.75 b | 92.97 ± 5.94 a | 90.80 ± 0.54 a | 97.48 ± 2.07 a | 9.537 | 0.001 |
Staphylococcaceae | 0.01 ± 0.00 b | 0.62 ± 0.44 b | 34.31 ± 11.58 a | 0.03 ± 0.02 b | 0.05 ± 0.01 b | 0.01 ± 0.00 b | 26.099 | <0.001 | |
Micrococcaceae | 0.17 ± 0.16 b | 4.62 ± 0.74 a | 3.84 ± 2.01 a | 0.05 ± 0.02 b | 0.01 ± 0.00 b | 0.01 ± 0.00 b | 18.391 | <0.001 | |
Enterobacteriaceae | 0.53 ± 0.23 b | 0.22 ± 0.03 bc | 0.34 ± 0.08 bc | 0.35 ± 0.23 bc | 2.38 ± 0.09 a | 0.04 ± 0.04 c | 110.248 | <0.001 | |
Erysipelatoclostridiaceae | 0.09 ± 0.01 b | 0.74 ± 0.11 a | 0.21 ± 0.06 b | 0.01 ± 0.00 b | 0.14 ± 0.23 b | 0.00 ± 0.00 b | 20.716 | <0.001 |
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Han, S.; Zhou, Y.; Wang, D.; Qin, Q.; Song, P.; He, Y. Effect of Different Host Plants on the Diversity of Gut Bacterial Communities of Spodoptera frugiperda (J. E. Smith, 1797). Insects 2023, 14, 264. https://doi.org/10.3390/insects14030264
Han S, Zhou Y, Wang D, Qin Q, Song P, He Y. Effect of Different Host Plants on the Diversity of Gut Bacterial Communities of Spodoptera frugiperda (J. E. Smith, 1797). Insects. 2023; 14(3):264. https://doi.org/10.3390/insects14030264
Chicago/Turabian StyleHan, Shipeng, Yayuan Zhou, Da Wang, Qiuju Qin, Peng Song, and Yunzhuan He. 2023. "Effect of Different Host Plants on the Diversity of Gut Bacterial Communities of Spodoptera frugiperda (J. E. Smith, 1797)" Insects 14, no. 3: 264. https://doi.org/10.3390/insects14030264