Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Butterfly Rearing
2.2. Experimental Operations
2.3. Fluorescent Probes
2.4. Confocal Imaging
3. Results
3.1. FB28 and SYBR Green I: Proximal Regions
3.2. FB28 and BODIPY: Distal Regions
3.3. FB28 and BODIPY: Middle Regions
3.4. Three-Dimensional Images
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nakazato, Y.; Otaki, J.M. Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns. Insects 2023, 14, 753. https://doi.org/10.3390/insects14090753
Nakazato Y, Otaki JM. Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns. Insects. 2023; 14(9):753. https://doi.org/10.3390/insects14090753
Chicago/Turabian StyleNakazato, Yugo, and Joji M. Otaki. 2023. "Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns" Insects 14, no. 9: 753. https://doi.org/10.3390/insects14090753