Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Identification of Neuropeptide Genes
2.2. Sample Collection
2.3. Liquid Chromatography
2.4. Mass Spectrometry
2.5. Data Analysis
3. Results
3.1. Differential Proteomics
3.2. KEGG Enrichment Analysis
3.3. Neuropeptide Hormones in the Midgut Proteome
4. Discussion
4.1. Neuropeptide Hormones
4.2. Impact of Starvation on Other Hormones
4.3. Metabolism and Digestive Enzymes
4.4. Feeding Variation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein ID | Protein Name | 24 h logFC | 48 h logFC | Combined logFC |
---|---|---|---|---|
Protein degradation | ||||
XP_030030816.2 | Mitochondrial-processing peptidase subunit beta | 1.5 | 2.1 | 1.8 |
XP_030031119.2 | Probable aminopeptidase NPEPL1 | 1.3 | 1.1 | 1.2 |
XP_030028976.2 | Zinc carboxypeptidase | −1.4 | −2.2 | −1.8 |
XP_030023348.2 | Carboxypeptidase B | −1.9 | −2.9 | −2.4 |
XP_030027851.2 | CAAX prenyl protease 1 homolog | −2.1 | −2.1 | −2.1 |
XP_037300887.1 | Antichymotrypsin-2-like | −2.4 | −4.0 | −3.2 |
XP_030024172.2 | Lysozyme | −2.3 | −4.8 | −3.6 |
XP_030021372.2 | Trypsin, alkaline C-like | −3.7 | −6.1 | −4.9 |
Sugar degradation | ||||
XP_030027415.1 | Maltase A1 | −2.0 | −4.2 | −3.1 |
XP_037295803.1 | Lactase–phlorizin hydrolase | −2.7 | −5.7 | −4.2 |
XP_037295801.1 | Lactase–phlorizin hydrolase | −4.2 | −7.2 | −5.7 |
Lipid degradation | ||||
XP_030034911.2 | Venom carboxylesterase 6 | 1.7 | 2.2 | 1.9 |
XP_030029542.2 | Pancreatic lipase-related protein 2-like | −4.3 | −4.6 | −4.4 |
Mitochondrial | ||||
XP_037294842.1 | Alanine–glyoxylate aminotransferase 2 | 2.6 | 3.1 | 2.8 |
XP_030027392.1 | Cytochrome c oxidase subunit 4 isoform 1 | 1.9 | 2.3 | 2.1 |
XP_030029341.1 | 39S ribosomal protein L41 | −1.1 | −1.2 | −1.1 |
XP_030024656.1 | 39S ribosomal protein L37 | −1.4 | −2.0 | −1.7 |
XP_030035113.2 | 3-ketoacyl-COA thiolase | −1.5 | −2.6 | −2.1 |
XP_030031455.1 | Mitochondrial glutamate carrier 1 | −2.1 | −3.0 | −2.6 |
XP_030026609.1 | Short-chain specific acyl-COA dehydrogenase | −3.0 | −2.7 | −2.9 |
XP_037294570.1 | 39S ribosomal protein s18a | −3.7 | −3.4 | −3.6 |
XP_037295058.1 | 3-ketoacyl-coa thiolase | −4.0 | −3.7 | −3.8 |
Metabolism | ||||
XP_030029916.1 | Diacylglycerol O-acyltransferase 1 | −1.4 | −1.6 | −1.5 |
XP_037292967.1 | Probable 3-hydroxyacyl-coa dehydrogenase B0272.3 | −1.3 | −1.9 | −1.6 |
XP_030023792.1 | Hydroxysteroid dehydrogenase-like protein 2 | −2.1 | −1.6 | −1.8 |
XP_030023969.1 | 3-hydroxyacyl-coa dehydrogenase type-2 | −1.8 | −1.9 | −1.9 |
XP_030032134.1 | Epidermal retinol dehydrogenase 2 | −2.3 | −1.7 | −2.0 |
XP_030028836.1 | D-altritol 5-dehydrogenase | −2.5 | −2.1 | −2.3 |
XP_030021537.1 | 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma | −2.6 | −2.0 | −2.3 |
XP_030026278.2 | Aldehyde dehydrogenase, dimeric NADP-preferring | −2.5 | −2.6 | −2.6 |
XP_030037221.2 | Estradiol 17-beta-dehydrogenase 8 | −2.9 | −3.4 | −3.2 |
XP_030038272.1 | 15-hydroxyprostaglandin dehydrogenase [NAD (+)] | −2.5 | −4.2 | −3.4 |
Juvenile hormone | ||||
XP_030040069.2 | Juvenile hormone esterase | 1.9 | 4.4 | 3.1 |
XP_037295223.1 | Juvenile hormone esterase-like | 1.9 | 2.8 | 2.4 |
XP_030032265.2 | Juvenile hormone epoxide hydrolase | −1.7 | −2.5 | −2.1 |
Cytochromes | ||||
XP_030034471.1 | Cytochrome b5 | 1.9 | 2.4 | 2.1 |
XP_030040484.1 | Cytochrome c-like | −1.1 | −1.4 | −1.3 |
XP_030025415.2 | NADH-cytochrome b5 reductase 2 | −1.4 | −1.3 | −1.3 |
Basement membrane assembly (laminin) | ||||
XP_030028135.1 | Laminin subunit beta-1 | 2.3 | 1.7 | 2.0 |
XP_037299829.1 | Laminin subunit gamma-1 | 2.4 | 1.7 | 2.0 |
XP_037299653.1 | Laminin subunit alpha-like | 2.1 | 1.7 | 1.9 |
Peptide | M. sexta Proteome | M. sexta Transcriptomes |
---|---|---|
Allatostatin A (helicostatins) | ++ | |
Allatostatin C | +++ | |
Bombyxin-related peptide A | na | +++ |
Bombyxin-related peptide A | na | + |
CCHamide-1 | + | |
CCHamide-2 | ++ | |
Corticotropin-releasing factor-like/diuretic hormone 44 | + | |
Diuretic hormone class 2/diuretic hormone 31/Calcitonin-like | + | |
Ecdysis-triggering hormone | + | |
IDLSRF-like peptide | + | |
Insulin-like growth-factor-binding protein complex acid labile subunit | + | +++ |
Insulin-like growth factor 2 mRNA-binding protein 1 | + | +++ |
Myosuppressin | + | +++ |
Neuropeptide-like 4 | ++++ | |
NPF1 (a and b) | + | +++ |
NPF2 | + | ++ |
Orcokinin | + | ++++ |
RYamide neuropeptides | + | |
sNPF | + | |
Tachykinin | +++ |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Kaur, G.; Quilici, D.R.; Woolsey, R.J.; Petereit, J.; Nuss, A.B. Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta. Insects 2024, 15, 325. https://doi.org/10.3390/insects15050325
Kaur G, Quilici DR, Woolsey RJ, Petereit J, Nuss AB. Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta. Insects. 2024; 15(5):325. https://doi.org/10.3390/insects15050325
Chicago/Turabian StyleKaur, Gurlaz, David R. Quilici, Rebekah J. Woolsey, Juli Petereit, and Andrew B. Nuss. 2024. "Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta" Insects 15, no. 5: 325. https://doi.org/10.3390/insects15050325