Ultrastructure of the Spermiogenesis in Halyomorpha halys (Hemiptera: Pentatomidae): X-Irradiation and New Insights on the Centriolar Region Organization
, Massimo Cristofaro 2, Gerardo Roselli 2,3, Raffaele Sasso 4, Sergio Musmeci 4, Francesco Barbieri 1, Chiara Sciandra 1,5, Valentina Vanoni 6, Loris Menegotti 6, Pio Federico Roversi 7, Gianfranco Anfora 3, David Mercati 5 and Romano Dallai 5,*Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe research provides an approach to the use of X-irradiation in insect control. Radiation demonstrates changes in the spermiogenesis process, especially in the organization of the centriole region.
The manuscript is incremental in theme. This approach is already present in the literature, but involving other groups of insects.
The manuscript provides a description of the impact of X-irradiation on the organization of the centriolar region, during the spermiogenesis process of a species of Pentatomidae (Heminoptera). As already mentioned, it is an incremental study adding data to the literature involving the action of X-irradiation in the spermiogenesis process, aiming at biological control of the species.
The methodology used was adequate for the defined purpose.
The conclusions described are compatible with the objective of the manuscript. The methodology used was appropriate, that is, the description of the spermiogenesis process in males subjected to X-irradiation. The results and discussion were presented in a clear and compatible manner.
The references used address the state of the art on the topic, being appropriate and current.
The images are relevant and appropriate to the manuscript’s approach. The excellent quality of transmission electron microscopy stands out.
Author Response
The research provides an approach to the use of X-irradiation in insect control. Radiation demonstrates changes in the spermiogenesis process, especially in the organization of the centriole region.
The manuscript is incremental in theme. This approach is already present in the literature, but involving other groups of insects.
The manuscript provides a description of the impact of X-irradiation on the organization of the centriolar region, during the spermiogenesis process of a species of Pentatomidae (Heminoptera). As already mentioned, it is an incremental study adding data to the literature involving the action of X-irradiation in the spermiogenesis process, aiming at biological control of the species.
The methodology used was adequate for the defined purpose.
The conclusions described are compatible with the objective of the manuscript. The methodology used was appropriate, that is, the description of the spermiogenesis process in males subjected to X-irradiation. The results and discussion were presented in a clear and compatible manner.
The references used address the state of the art on the topic, being appropriate and current.
The images are relevant and appropriate to the manuscript’s approach. The excellent quality of transmission electron microscopy stands out.
We thank the reviewer for his kind comments on our work
Reviewer 2 Report
Comments and Suggestions for AuthorsThe manuscript #insects-3079676 deals with the spermiogenesis of the important stink bug pest Halyomorpha halys. Generally speaking, the pictures are of good quality, but the manuscript is well written. This paper is a great contribution to the sperm morphology of insects. Therefore, I recommend to publish this paper in Insects after a minor revision.
Abstract. Line 51: “failure to reabsorption the cytoplasm”. Here, I think a verb (reabsorb) rather than a noun should be used.
Results. L178, L283, and L304: The period (.) before the bracket should be deleted.
Figure 2. L188: There are six follicles. Two are large, one is median, therefore, three (rather than two” should be small. Please modify properly.
L278: “between A and B”, and “from A to B”, but not “between A to B”. “Thus, to increase image quality and allow a high-resolution power for each mounted histological image” lack a complete sentence structure, therefore should be rewritten.
Figure 9. L322: “free the in” should be modified as “free in the”.
L381: The scientific name “H. halys” should be in italics.
Comments on the Quality of English LanguageThe English Language of this paper is of high quality.
Author Response
Abstract. Line 51: “failure to reabsorption the cytoplasm”. Here, I think a verb (reabsorb) rather than a noun should be used.
Results. L178, L283, and L304: The period (.) before the bracket should be deleted.
Figure 2. L188: There are six follicles. Two are large, one is median, therefore, three (rather than two” should be small. Please modify properly.
L278: “between A and B”, and “from A to B”, but not “between A to B”. “Thus, to increase image quality and allow a high-resolution power for each mounted histological image” lack a complete sentence structure, therefore should be rewritten.
Figure 9. L322: “free the in” should be modified as “free in the”.
L381: The scientific name “H. halys” should be in italics.
We followed all the suggestions given to us by the Reviewer
