Identification and Functional Analysis of V-ATPaseA and C Genes in Hyphantria cunea
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Cloning and Validation of cDNA Seqences of HcV-ATPases
2.3. Bioinformatics Analysis of HcV-ATPases
2.4. Expression Pattern of HcV-ATPases in Different Developmental Stages and Tissues
2.5. Functional Analysis of HcV-ATPases by RNAi
2.6. H&E Staining and Transmission Electron Microscopy of Midgut
2.7. Immunohistochemistry
2.8. Statistical Analysis
3. Results
3.1. Identification and Characterization of HcV-ATPase A and C
3.2. Expression Patterns of HcV-ATPase A and C
3.3. Effect on H. cunea Survival after HcV-ATPase A and C RNAi
3.4. Immunohistochemistry
3.5. Effect of RNAi of HcV-ATPase A and C on H. cunea Midgut
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Appendix A
Primer Name | Sequence (5′-3′) | Application | Product Size (bp) |
---|---|---|---|
HcV-ATPase AF | CCGGAATTCATGGCGTCGGATTATCAG | cDNA verification | 1845 |
HcV-ATPase AR | CCAAGCTTCTTAGTCCTCGAGGTTGCG | ||
HcV-ATPase CF | CCGGAATTCATGTCTGAATACTGGGTGA | 1158 | |
HcV-ATPase CR | CGCGTCGACCATTAGTTCGCCTTCTCG | ||
qHcV-ATPase AF | GTGATGTCAGCTGGGAATT | qRT-PCR | 156 |
qHcV-ATPase AR | AGAAGGCGCGATGTATGT | ||
qHcV-ATPase CF | CTGATGACCTTGGTAAGCTTG | 154 | |
qHcV-ATPase CR | TATTTCGCCATGTCCCAT | ||
actinF | CTACCTCACGCCATTCTC | 150 | |
actinR | AGCTTCTCCTTGATGTCAC | ||
dsHcV-ATPase AF | TAATACGACTCACTATAGGGAGAAGATGCCGGCCAAC | RNAi | 527 |
dsHcV-ATPase AR | TAATACGACTCACTATAGGCGTCCAGCTCGCTCGTAG | ||
dsHcV-ATPase CF | TAATACGACTCACTATAGGCCTACAATGCACTCAAGGGC | 502 | |
dsHcV-ATPase CR | TAATACGACTCACTATAGGGATCCAGGCGCAGAAACAC | ||
dsGFPF | TAATACGACTCACTATAGGCCACAAGTTCAGCGTGT | 500 | |
dsGFPR | TAATACGACTCACTATAGGAGTTCACCTTGATGCCG |
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Wang, X.; Zhao, D.; Wang, Q.; Liu, Y.; Lu, X.; Guo, W. Identification and Functional Analysis of V-ATPaseA and C Genes in Hyphantria cunea. Insects 2024, 15, 515. https://doi.org/10.3390/insects15070515
Wang X, Zhao D, Wang Q, Liu Y, Lu X, Guo W. Identification and Functional Analysis of V-ATPaseA and C Genes in Hyphantria cunea. Insects. 2024; 15(7):515. https://doi.org/10.3390/insects15070515
Chicago/Turabian StyleWang, Xiaojie, Dan Zhao, Qian Wang, Yanan Liu, Xiujun Lu, and Wei Guo. 2024. "Identification and Functional Analysis of V-ATPaseA and C Genes in Hyphantria cunea" Insects 15, no. 7: 515. https://doi.org/10.3390/insects15070515