Identification and Functional Insights of Knickkopf Genes in the Larval Cuticle of Leptinotarsa decemlineata
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Insect
2.2. Identification of LdKnk-Family Genes
2.3. Bioinformatics and Phylogenetic Analysis
2.4. Expression Analysis of LdKnk-Family Genes
2.5. Preparation of dsRNA Using Bacterial Expression
2.6. Dietary dsRNA Bioassays
2.7. Transmission Electron Microscopy (TEM) Analysis
2.8. Data Analysis
3. Results
3.1. Identification and Characterization of LdKnk-Family Genes in L.decemlineata
3.2. Spatio-Temporal Expression Patterns of LdKnk-Family Genes
3.3. Effects of Silencing LdKnk Genes on Larval Development and Epidermis Structure
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Accession Number | cDNA (bp) | Coding Region | 5′-UTR (bp) | 3′-UTR (bp) | aa | pl | Mw(kDa) |
---|---|---|---|---|---|---|---|---|
LdKnk | PP962378 | 2118 | 61–2088 | 60 | 30 | 675 | 6.35 | 76.03 |
LdKnk2 | PP962379 | 2142 | 1–2109 | 0 | 33 | 702 | 5.09 | 78.90 |
LdKnk3-FL | PP962380 | 5100 | 49–4236 | 48 | 864 | 1395 | 7.41 | 156.48 |
LdKnk3-5′ | PP962381 | 1042 | 61–975 | 60 | 67 | 304 | 7.65 | 33.81 |
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Zeng, M.-Z.; Zhou, W.; Wen, S.-S.; Wu, H.; Zhang, Q.; Fu, K.-Y.; Guo, W.-C.; Shi, J.-F. Identification and Functional Insights of Knickkopf Genes in the Larval Cuticle of Leptinotarsa decemlineata. Insects 2024, 15, 623. https://doi.org/10.3390/insects15080623
Zeng M-Z, Zhou W, Wen S-S, Wu H, Zhang Q, Fu K-Y, Guo W-C, Shi J-F. Identification and Functional Insights of Knickkopf Genes in the Larval Cuticle of Leptinotarsa decemlineata. Insects. 2024; 15(8):623. https://doi.org/10.3390/insects15080623
Chicago/Turabian StyleZeng, Mu-Zi, Wei Zhou, Shan-Shan Wen, Hao Wu, Qing Zhang, Kai-Yun Fu, Wen-Chao Guo, and Ji-Feng Shi. 2024. "Identification and Functional Insights of Knickkopf Genes in the Larval Cuticle of Leptinotarsa decemlineata" Insects 15, no. 8: 623. https://doi.org/10.3390/insects15080623