Insects

623 KiB

Open AccessArticle

Optimizing Western Flower Thrips Management on French Beans by Combined Use of Beneficials and Imidacloprid

by Johnson O. Nyasani, Sevgan Subramanian, Hans-Michael Poehling, Nguya K. Maniania, Sunday Ekesi and Rainer Meyhöfer

Insects 2015, 6(1), 279-296; https://doi.org/10.3390/insects6010279 - 23 Mar 2015

Cited by 14 | Viewed by 7499

Abstract

Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an important pest of vegetable crops worldwide and has developed resistance to many insecticides. The predatory mites Neoseiulus (=Amblyseius) cucumeris (Oudemans), the entomopathogenic fungus Metarhizium anisopliae (Metsch.), and an insecticide (imidacloprid) were tested [...] Read more.

Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an important pest of vegetable crops worldwide and has developed resistance to many insecticides. The predatory mites Neoseiulus (=Amblyseius) cucumeris (Oudemans), the entomopathogenic fungus Metarhizium anisopliae (Metsch.), and an insecticide (imidacloprid) were tested for their efficacy to reduce WFT population density and damage to French bean (Phaseolus vulgaris L.) pods under field conditions in two planting periods. Metarhizium anisopliae was applied as a foliar spray weekly at a rate of one litre spray volume per plot while imidacloprid was applied as a soil drench every two weeks at a rate of two litres of a mixture of water and imidacloprid per m². Neoseiulus cucumeris was released every two weeks on plant foliage at a rate of three mites per plant. Single and combined treatment applications reduced WFT population density by at least three times and WFT damage to French bean pods by at least 1.7 times compared with untreated plots. The benefit-cost ratios in management of WFT were profitable with highest returns realized on imidacloprid treated plots. The results indicate that M. anisopliae, N. cucumeris, and imidacloprid have the potential for use in developing an integrated pest management program against WFT on French beans. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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359 KiB

Open AccessReview

Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes

by Joy Kean, Stephanie M. Rainey, Melanie McFarlane, Claire L. Donald, Esther Schnettler, Alain Kohl and Emilie Pondeville

Insects 2015, 6(1), 236-278; https://doi.org/10.3390/insects6010236 - 23 Mar 2015

Cited by 58 | Viewed by 11964

Abstract

Control of aedine mosquito vectors, either by mosquito population reduction or replacement with refractory mosquitoes, may play an essential role in the fight against arboviral diseases. In this review, we will focus on the development and application of biological approaches, both natural or [...] Read more.

Control of aedine mosquito vectors, either by mosquito population reduction or replacement with refractory mosquitoes, may play an essential role in the fight against arboviral diseases. In this review, we will focus on the development and application of biological approaches, both natural or engineered, to limit mosquito vector competence for arboviruses. The study of mosquito antiviral immunity has led to the identification of a number of host response mechanisms and proteins that are required to control arbovirus replication in mosquitoes, though more factors influencing vector competence are likely to be discovered. We will discuss key aspects of these pathways as targets either for selection of naturally resistant mosquito populations or for mosquito genetic manipulation. Moreover, we will consider the use of endosymbiotic bacteria such as Wolbachia, which in some cases have proven to be remarkably efficient in disrupting arbovirus transmission by mosquitoes, but also the use of naturally occurring insect-specific viruses that may interfere with arboviruses in mosquito vectors. Finally, we will discuss the use of paratransgenesis as well as entomopathogenic fungi, which are also proposed strategies to control vector competence. Full article

(This article belongs to the Special Issue Mosquito Control)

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216 KiB

Open AccessArticle

Susceptibility of Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) to Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae and Steinernematidae)

by Joseph Tourtois and Matthew J. Grieshop

Insects 2015, 6(1), 224-235; https://doi.org/10.3390/insects6010224 - 18 Mar 2015

Cited by 3 | Viewed by 5848

Abstract

Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) and entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) are two soil-dwelling biological control agents used to manage western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and fungus gnats Bradysis spp. (Diptera: Sciaridae) in glasshouses. Growers often use multiple natural [...] Read more.

Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) and entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) are two soil-dwelling biological control agents used to manage western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and fungus gnats Bradysis spp. (Diptera: Sciaridae) in glasshouses. Growers often use multiple natural enemies to achieve economic control, but knowledge of interactions among natural enemies is lacking. We conducted a laboratory bioassay to test the pathogenicity of four commercially available nematode species—Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhbditidae), Steinernema carpocapsae (Weiser) (Rhabditida: Steinernematidae), S. feltiae (Filipjev), and S. riobrave Cabanillas et al.—to third instar and adult D. coriaria. Third instars were three times more susceptible than the adults to the entomopathogenic nematodes. Mortality for D. coriaria adults and third instars treated with S. feltiae and H. bacteriophora was lower than the mortality for D. coriaria adults and third instars treated with S. carpocapsae and S. riobrave. Neither infective juvenile foraging behavior nor size correlates with D. coriaria mortality. Dalotia coriaria appears to be most likely compatible with applications of S. feltiae and H. bacteriophora. Full article

(This article belongs to the Special Issue Pest Control in Glasshouses)

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Open AccessArticle

Improvement of the Insecticidal Capacity of Two Purpureocillium Lilacinum Strains against Tribolium Confusum

by Paula Barra, Miriam Etcheverry and Andrea Nesci

Insects 2015, 6(1), 206-223; https://doi.org/10.3390/insects6010206 - 18 Mar 2015

Cited by 15 | Viewed by 5963

Abstract

Entomopathogenic fungi can regulate insect populations. They have extracellular enzymes that degrade cuticle components, mainly hydrocarbons, used as an energy source. The increase in insecticidal activity of fungi in a medium supplemented with cuticular hydrocarbons was assayed and the hydrolytic enzyme profiles of [...] Read more.

Entomopathogenic fungi can regulate insect populations. They have extracellular enzymes that degrade cuticle components, mainly hydrocarbons, used as an energy source. The increase in insecticidal activity of fungi in a medium supplemented with cuticular hydrocarbons was assayed and the hydrolytic enzyme profiles of two strains of Purpureocillium lilacinum were evaluated. A spore suspension of P. lilacinum was inoculated in Petri plates with different values (0.99–0.97–0.95) of water activity (Aw) using the substrates gelatin, starch and tween-20. Growth rate on the different substrates and the enzymatic activity index for proteases, amylases and lipases at different incubation times, pH and Aw, was evaluated. Moreover, the insecticidal efficiency of strains grown in media supplemented with n-hexadecane and n-octacosane was analyzed. LT₅₀ was calculated against adults of Tribolium confusum and showed that mortality increased about 15% when the strains grew in amended culture medium. High amylolytic activity was detected, but proteases were the main enzymes produced. Optimal protease production was observed in a range of acid and alkaline pH and lower Aw. The greatest growth rate was obtained in presence of gelatin. Lipase and amylase production was detected in small amounts. Fungal growth in media with hydrocarbon mixtures increased the pathogenicity of the two strains of P. lilacinum, with the strain JQ926223 being more virulent. The information obtained is important for achieving both an increase in insecticidal capacity and an understanding of physiological adaptation of the fungus. Full article

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Open AccessArticle

Field Study of the Comparative Efficacy of Three Pyrethroid/Neonicotinoid Mixture Products for the Control of the Common Bed Bug, Cimex lectularius

by Changlu Wang, Narinderpal Singh and Richard Cooper

Insects 2015, 6(1), 197-205; https://doi.org/10.3390/insects6010197 - 18 Mar 2015

Cited by 28 | Viewed by 7454

Abstract

Three insecticide mixtures that contain two classes of insecticides (pyrethroid and neonicotinoid) were recently developed to control bed bugs. We evaluated three integrated bed bug management strategies in apartments, each using the same non-chemical control methods and one of the three insecticide mixture [...] Read more.

Three insecticide mixtures that contain two classes of insecticides (pyrethroid and neonicotinoid) were recently developed to control bed bugs. We evaluated three integrated bed bug management strategies in apartments, each using the same non-chemical control methods and one of the three insecticide mixture products: Tandem (lambda-cyhalothrin + thiamethoxam), Temprid SC (beta-cyfluthrin + imidacloprid), and Transport Mikron (bifenthrin + acetamiprid). No insecticides were applied in the Control apartments. In all apartments, we installed vinyl mattress encasements (if not already present) and applied steam to beds and other infested upholstered furniture. Insecticide sprays were applied in the three treatments. Each treatment and the Control included 8–10 occupied apartments. Re-treatment was conducted during biweekly inspections if necessary. After eight weeks, the mean (± SEM) bed bug count reduction in the Tandem, Temprid SC, Transport Mikron, and Control was 89 ± 9, 87 ± 6, 98 ± 1, and 23 ± 54%, respectively. Only Tandem and Transport Mikron treatments resulted in significantly higher population reduction than the Control at eight weeks. There were no significant differences in mean percent reduction among the three treatments (Tandem, Temprid SC, Transport Mikron) at eight weeks. Tandem spray caused significantly faster bed bug reduction than Temprid SC spray and Transport Mikron spray. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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Open AccessArticle

Sexual Behavior of Drosophila suzukii

by Santosh Revadi, Sébastien Lebreton, Peter Witzgall, Gianfranco Anfora, Teun Dekker and Paul G. Becher

Insects 2015, 6(1), 183-196; https://doi.org/10.3390/insects6010183 - 9 Mar 2015

Cited by 81 | Viewed by 17141

Abstract

A high reproductive potential is one reason for the rapid spread of Drosophila suzukii in Europe and in the United States. In order to identify mechanisms that mediate mating and reproduction in D. suzukii we studied the fly’s reproductive behavior, diurnal mating activity [...] Read more.

A high reproductive potential is one reason for the rapid spread of Drosophila suzukii in Europe and in the United States. In order to identify mechanisms that mediate mating and reproduction in D. suzukii we studied the fly’s reproductive behavior, diurnal mating activity and sexual maturation. Furthermore, we studied the change of female cuticular hydrocarbons (CHCs) with age and conducted a preliminary investigation on the role of female-derived chemical signals in male mating behavior. Sexual behavior in D. suzukii is characterized by distinct elements of male courtship leading to female acceptance for mating. Time of day and age modulate D. suzukii mating activity. As with other drosophilids, female sexual maturity is paralleled by a quantitative increase in CHCs. Neither female CHCs nor other olfactory signals were required to induce male courtship, however, presence of those signals significantly increased male sexual behavior. With this pilot study we hope to stimulate research on the reproductive biology of D. suzukii, which is relevant for the development of pest management tools. Full article

(This article belongs to the Special Issue Pheromones and Insect Behaviour)

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252 KiB

Open AccessArticle

Integrated Pest Management for Sustainable Intensification of Agriculture in Asia and Africa

by Jules Pretty and Zareen Pervez Bharucha

Insects 2015, 6(1), 152-182; https://doi.org/10.3390/insects6010152 - 5 Mar 2015

Cited by 360 | Viewed by 54870

Abstract

Integrated Pest Management (IPM) is a leading complement and alternative to synthetic pesticides and a form of sustainable intensification with particular importance for tropical smallholders. Global pesticide use has grown over the past 20 years to 3.5 billion kg/year, amounting to a global [...] Read more.

Integrated Pest Management (IPM) is a leading complement and alternative to synthetic pesticides and a form of sustainable intensification with particular importance for tropical smallholders. Global pesticide use has grown over the past 20 years to 3.5 billion kg/year, amounting to a global market worth $45 billion. The external costs of pesticides are $4–$19 (€3–15) per kg of active ingredient applied, suggesting that IPM approaches that result in lower pesticide use will benefit, not only farmers, but also wider environments and human health. Evidence for IPM’s impacts on pesticide use and yields remains patchy. We contribute an evaluation using data from 85 IPM projects from 24 countries of Asia and Africa implemented over the past twenty years. Analysing outcomes on productivity and reliance on pesticides, we find a mean yield increase across projects and crops of 40.9% (SD 72.3), combined with a decline in pesticide use to 30.7% (SD 34.9) compared with baseline. A total of 35 of 115 (30%) crop combinations resulted in a transition to zero pesticide use. We assess successes in four types of IPM projects, and find that at least 50% of pesticide use is not needed in most agroecosystems. Nonetheless, policy support for IPM is relatively rare, counter-interventions from pesticide industry common, and the IPM challenge never done as pests, diseases and weeds evolve and move. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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Open AccessArticle

Foliar Substrate Affects Cuticular Hydrocarbon Profiles and Intraspecific Aggression in the Leafcutter Ant Atta sexdens

by Lohan Valadares, Daniela Nascimento and Fabio S. Nascimento

Insects 2015, 6(1), 141-151; https://doi.org/10.3390/insects6010141 - 12 Feb 2015

Cited by 15 | Viewed by 8249

Abstract

Cuticular hydrocarbons (CHCs) are traditionally considered to be one of the most important chemical cues used in the nestmate recognition process of social hymenopterans. However, it has been suggested that in the leafcutter ant genus Atta, it is not the CHCs, but [...] Read more.

Cuticular hydrocarbons (CHCs) are traditionally considered to be one of the most important chemical cues used in the nestmate recognition process of social hymenopterans. However, it has been suggested that in the leafcutter ant genus Atta, it is not the CHCs, but the alarm pheromone that is involved in the nestmate recognition process. In this study we used a laboratory population of Atta sexdens to explore the association between their CHC profile variation and intraspecific aggression. In the first part of the experiment, four colonies were divided into two groups with distinct diets to stimulate differentiation of their CHC profiles. In the second part of the experiment, all colonies received the same diet to examine resemblance of chemical profiles. At the end of each part of the experiment we extracted the CHCs from workers. The results demonstrated that colonies that shared the same food resource had similar cuticular hydrocarbon profiles. Furthermore, colonies were significantly more aggressive towards conspecifics that used a different foliar substrate and consequently had greater differences in their cuticular chemical composition. This study suggests that the CHC profiles of A. sexdens can be affected by the foliar substrates used, and that the CHCs are used in the nestmate recognition process of this species. Full article

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Open AccessArticle

Tri-Tek (Petroleum Horticultural Oil) and Beauveria bassiana: Use in Eradication Strategies for Bemisia tabaci Mediterranean Species in UK Glasshouses

by Andrew G. S. Cuthbertson and Debbie A. Collins

Insects 2015, 6(1), 133-140; https://doi.org/10.3390/insects6010133 - 12 Feb 2015

Cited by 9 | Viewed by 6217

Abstract

The sweetpotato whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. To date, B. tabaci has not become established in the UK. The UK holds Protected Zone status against this pest and, as a [...] Read more.

The sweetpotato whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. To date, B. tabaci has not become established in the UK. The UK holds Protected Zone status against this pest and, as a result, B. tabaci entering on plant material is subjected to a policy of eradication. Mediterranean species is now the most prevalent Bemisia species entering the UK. Increasing neonicotinoid resistance is becoming increasingly widespread and problematic with this species. As a result, this continues to pose problems for eradication strategies. The current study investigates the efficacy of Tri-Tek (a petroleum horticultural oil awaiting UK registration) and the fungus Beauveria bassiana to act as control agents against Mediterranean species in UK glasshouses. Tri-Tek provided 100% egg mortality compared to 74% for B. bassiana. When tested against second instar larvae, mortalities of 69% and 65% respectively were achieved. Both products can be successfully “tank-mixed”. A tank-mix application provided 95.5% mortality of second instar larvae under glasshouse conditions. The potential integration of both products into current Bemisia eradication strategies in UK glasshouses is discussed. Full article

(This article belongs to the Special Issue Pest Control in Glasshouses)

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225 KiB

Open AccessArticle

Insecticide Resistance in Eggs and First Instars of the Bed Bug, Cimex lectularius (Hemiptera: Cimicidae)

by Brittany E. Campbell and Dini M. Miller

Insects 2015, 6(1), 122-132; https://doi.org/10.3390/insects6010122 - 15 Jan 2015

Cited by 21 | Viewed by 6949

Abstract

Two strains of the common bed bug, Cimex lectularius L., eggs and first instars collected from pyrethroid-resistant adults were evaluated for insecticide resistance and compared to a susceptible strain. Dose-response bioassays were conducted using two insecticide formulations (Temprid: imidacloprid/β-cyfluthrin, and Transport: acetamiprid/ bifenthrin). [...] Read more.

Two strains of the common bed bug, Cimex lectularius L., eggs and first instars collected from pyrethroid-resistant adults were evaluated for insecticide resistance and compared to a susceptible strain. Dose-response bioassays were conducted using two insecticide formulations (Temprid: imidacloprid/β-cyfluthrin, and Transport: acetamiprid/ bifenthrin). The lethal concentration (LC₅₀) for the two resistant egg strains exposed to imidacloprid/β-cyfluthrin ranged from 3 to 5-fold higher than susceptible strain eggs. Resistant strain eggs dipped into formulations of acetamiprid/bifenthrin had LC₅₀ values which were significantly greater (39 to 1,080-fold) than susceptible strain eggs. Similar to eggs, resistant strain first instars exposed to residual applications of imidacloprid/β-cyfluthrin had LC₅₀ values ranging from 121 to 493-fold greater than susceptible strain first instars. When resistant strain first instars were treated with acetamiprid/bifenthrin, they had LC₅₀ values that were 99 to >1,900-fold greater than susceptible strain first instars. To determine differences between egg and first instar resistance, stage resistance ratios (SRR) were compared between the two stages. There was little difference between the egg and first instar stages, indicated by small SRR values ranging from 1.1 to 10.0. This study suggests that insecticide resistance is expressed early during bed bug development. Full article

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Open AccessArticle

Do Offspring of Insects Feeding on Defoliation-Resistant Trees Have Better Biological Performance When Exposed to Nutritionally-Imbalanced Food?

by Roberto Quezada-Garcia, Alvaro Fuentealba, Ngoc Nguyen and Éric Bauce

Insects 2015, 6(1), 112-121; https://doi.org/10.3390/insects6010112 - 12 Jan 2015

Cited by 1 | Viewed by 4979

Abstract

White spruce (Picea glauca (Moench) Voss) trees that are resistant or susceptible to spruce budworm (Choristoneura fumiferana (Clem.)) attack were identified in a southern Quebec plantation. Due to high mortality-induced selective pressures imposed by resistant trees on spruce budworm larvae, insects [...] Read more.

White spruce (Picea glauca (Moench) Voss) trees that are resistant or susceptible to spruce budworm (Choristoneura fumiferana (Clem.)) attack were identified in a southern Quebec plantation. Due to high mortality-induced selective pressures imposed by resistant trees on spruce budworm larvae, insects that survive on resistant trees exhibited greater biological performance than those on susceptible trees. We tested the hypothesis that this better biological performance is maintained across generations when progeny were subjected to nutritional stress. We collected pupae from resistant and susceptible trees (phenotype). Adults were reared under controlled laboratory conditions. Progeny were subsequently reared on two types of artificial diet (high vs. low quality). Low quality diet simulated food quality deterioration during outbreak conditions. Results confirmed that surviving insects collected from resistant trees have better performance than those from susceptible trees. Offspring performance (pupal mass, developmental time) was affected only by diet quality. These results suggest that adaptive advantages that would be acquired from parents fed on resistant trees are lost when progeny are exposed to nutritionally-imbalanced food, but the effects persist when larvae are fed a balanced diet. Offspring mortality, fecundity and fertility were positively influenced by parental origin (tree phenotype). Full article

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Open AccessArticle

Effects of Starvation on Deltamethrin Tolerance in Bed Bugs, Cimex lectularius L. (Hemiptera: Cimicidae)

by Zachary C. DeVries, William R. Reid, Stephen A. Kells and Arthur G. Appel

Insects 2015, 6(1), 102-111; https://doi.org/10.3390/insects6010102 - 9 Jan 2015

Cited by 11 | Viewed by 5638

Abstract

Bed bugs, Cimex lectularius L., are a major pest in the urban environment. Their presence often results in physical, psychological, and financial distress of homeowners and apartment dwellers. Although many insecticide bioassays have been performed on this pest, little attention has been paid [...] Read more.

Bed bugs, Cimex lectularius L., are a major pest in the urban environment. Their presence often results in physical, psychological, and financial distress of homeowners and apartment dwellers. Although many insecticide bioassays have been performed on this pest, little attention has been paid to bed bug feeding status, which is closely linked to metabolism, molting, and mass. Therefore, we evaluated the toxicity of topically applied deltamethrin on insecticide susceptible adult male bed bugs fed 2 d, 9 d, and 21 d prior to testing. When toxicity was evaluated on a “per-bug” basis, there was no difference between 2 d [LD₅₀ = 0.498 (0.316 − 0.692) ng·bug⁻¹] and 9 d [LD₅₀ = 0.572 (0.436 − 0.724) ng·bug⁻¹] starved bugs, while 21 d starved bugs had a significantly lower LD₅₀ [0.221 (0.075 − 0.386) ng·bug⁻¹]. When toxicity was evaluated in terms of body mass, 9 d starved bugs had the highest LD₅₀ values [0.138 (0.102 − 0.176) ng·mg⁻¹], followed by 2 d starved bugs [0.095 (0.060 − 0.134) ng·mg⁻¹], and then 21 d starved bugs [0.058 (0.019–0.102) ng·mg⁻¹]; the LD₅₀ values of 2 d and 9 d starved bugs were significantly different from 21 d starved bugs. These results indicate that feeding status plays an important role in the toxicity of deltamethrin. In addition, the lack of differences between 2 d and 9 d starved bugs indicate that the blood meal itself has little impact on tolerance, but rather it is some physiological change following feeding that confers increased tolerance to bed bugs. Full article

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Open AccessEditorial

Acknowledgement to Reviewers of Insects in 2014

by Insects Editorial Office

Insects 2015, 6(1), 100-101; https://doi.org/10.3390/insects6010100 - 8 Jan 2015

Viewed by 3468

Abstract

The editors of Insects would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2014:[...] Full article

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Open AccessArticle

Male Armaments and Reproductive Behavior in “Nutcracker” Camel Crickets (Rhaphidophoridae, Pristoceuthophilus)

by Lauren P. Conroy and David A. Gray

Insects 2015, 6(1), 85-99; https://doi.org/10.3390/insects6010085 - 7 Jan 2015

Cited by 9 | Viewed by 6958

Abstract

Males of many species possess striking weaponry used in intrasexual competition for access to females. Until recently, there were no known cases of male weaponry being used against females in sexual coercion. However, in the camel cricket, Pristoceuthophilus marmoratus, males use modified [...] Read more.

Males of many species possess striking weaponry used in intrasexual competition for access to females. Until recently, there were no known cases of male weaponry being used against females in sexual coercion. However, in the camel cricket, Pristoceuthophilus marmoratus, males use modified hind legs to fight with each other and also to trap females and force them to copulate. To determine whether hind leg armaments serve similar fighting and mating functions in morphologically similar congeners, we performed a comparative survey of armament use in intra- and inter-sexual interactions in four additional species of Pristoceuthophilus (P. arizonae and three undescribed species: P. ‘Huachuca summer,’ P. ‘Madera’ and P. ‘Mt. Pinos’). Intrasexual leg fighting occurred in all species for which trials were performed, and hints of sexual coercion occurred in two species (P. ‘Huachuca summer’ and P. ‘Mt. Pinos’), suggesting additional cases of a uniquely dual-purpose armament. These findings suggest an evolutionary exaptation of hind leg armaments in this genus, wherein an intrasexual fighting weapon took on a secondary function of sexual coercion. Full article

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Open AccessArticle

Control of Cowpea Weevil, Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), Using Natural Plant Products

by Bamphitlhi Tiroesele, Kesegofetse Thomas and Seipati Seketeme

Insects 2015, 6(1), 77-84; https://doi.org/10.3390/insects6010077 - 31 Dec 2014

Cited by 41 | Viewed by 11602

Abstract

A laboratory study was conducted to investigate the effects of natural products on the reproduction and damage of Callosobruchus maculatus, the cowpea weevil, on cowpea seeds at Botswana College of Agriculture in Gaborone, Botswana. The cowpea variety Blackeye was used in the study. [...] Read more.

A laboratory study was conducted to investigate the effects of natural products on the reproduction and damage of Callosobruchus maculatus, the cowpea weevil, on cowpea seeds at Botswana College of Agriculture in Gaborone, Botswana. The cowpea variety Blackeye was used in the study. Fifty grams of each plant product (garlic, peppermint and chilies) was added to 500 g of the cowpea seeds. Findings of this experiment revealed that chilies and garlic had negative effects on cowpea weevils for all parameters measured. Peppermint also showed significant reduction in the F₁ progeny of the cowpea weevils but with less effect on weevils than garlic and chilies. The results indicate that these plant products have the potential to protect cowpea seeds from cowpea weevils’ damage compared to when the seeds are left or stored unprotected. They should, therefore, be included in pest management strategies for cowpea weevil in grains stored on-farm in rural tropical and subtropical regions. Full article

(This article belongs to the Special Issue Insect-Plant Interactions)

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Open AccessArticle

Molecular Cloning and Characterization of G Alpha Proteins from the Western Tarnished Plant Bug, Lygus hesperus

by J. Joe Hull and Meixian Wang

Insects 2015, 6(1), 54-76; https://doi.org/10.3390/insects6010054 - 31 Dec 2014

Cited by 2 | Viewed by 6915

Abstract

The Gα subunits of heterotrimeric G proteins play critical roles in the activation of diverse signal transduction cascades. However, the role of these genes in chemosensation remains to be fully elucidated. To initiate a comprehensive survey of signal transduction genes, we used homology-based [...] Read more.

The Gα subunits of heterotrimeric G proteins play critical roles in the activation of diverse signal transduction cascades. However, the role of these genes in chemosensation remains to be fully elucidated. To initiate a comprehensive survey of signal transduction genes, we used homology-based cloning methods and transcriptome data mining to identity Gα subunits in the western tarnished plant bug (Lygus hesperus Knight). Among the nine sequences identified were single variants of the Gαi, Gαo, Gαs, and Gα12 subfamilies and five alternative splice variants of the Gαq subfamily. Sequence alignment and phylogenetic analyses of the putative L. hesperus Gα subunits support initial classifications and are consistent with established evolutionary relationships. End-point PCR-based profiling of the transcripts indicated head specific expression for LhGαq4, and largely ubiquitous expression, albeit at varying levels, for the other LhGα transcripts. All subfamilies were amplified from L. hesperus chemosensory tissues, suggesting potential roles in olfaction and/or gustation. Immunohistochemical staining of cultured insect cells transiently expressing recombinant His-tagged LhGαi, LhGαs, and LhGαq1 revealed plasma membrane targeting, suggesting the respective sequences encode functional G protein subunits. Full article

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Open AccessReview

Aggression in Tephritidae Flies: Where, When, Why? Future Directions for Research in Integrated Pest Management

by Giovanni Benelli

Insects 2015, 6(1), 38-53; https://doi.org/10.3390/insects6010038 - 30 Dec 2014

Cited by 28 | Viewed by 9884

Abstract

True fruit flies (Diptera: Tephritidae) include over 4000 species, many of which constitute enormous threats to fruit and vegetable production worldwide. A number of Tephritidae are lekking species, forming aggregations in which males fight to defend a small territory where they court females [...] Read more.

True fruit flies (Diptera: Tephritidae) include over 4000 species, many of which constitute enormous threats to fruit and vegetable production worldwide. A number of Tephritidae are lekking species, forming aggregations in which males fight to defend a small territory where they court females and mate. Male-male contests also occur in non-lekking species, characterized by resource defense polygyny. Tephritidae females display agonistic behavior to maintain single oviposition sites and reduce larval competition for food. Here, how, where, when and why aggressive interactions occur in Tephritidae flies is reviewed. A number of neglected issues deserving further research are highlighted, with a special focus on diel periodicity of aggression, cues evoking aggressive behavior, the role of previous experience on fighting success and the evolution of behavioral lateralization of aggressive displays. In the final section, future directions to exploit this knowledge in Integrated Pest Management, with particular emphasis on enhancement of Sterile Insect Technique and interspecific competitive displacement in the field are suggested. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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Open AccessReview

Classical Biological Control of Invasive Legacy Crop Pests: New Technologies Offer Opportunities to Revisit Old Pest Problems in Perennial Tree Crops

by Mark S. Hoddle, Keith Warner, John Steggall and Karen M. Jetter

Insects 2015, 6(1), 13-37; https://doi.org/10.3390/insects6010013 - 23 Dec 2014

Cited by 20 | Viewed by 9025

Abstract

Advances in scientific disciplines that support classical biological control have provided “new tools” that could have important applications for biocontrol programs for some long-established invasive arthropod pests. We suggest that these previously unavailable tools should be used in biological control programs targeting “legacy [...] Read more.

Advances in scientific disciplines that support classical biological control have provided “new tools” that could have important applications for biocontrol programs for some long-established invasive arthropod pests. We suggest that these previously unavailable tools should be used in biological control programs targeting “legacy pests”, even if they have been targets of previously unsuccessful biocontrol projects. Examples of “new tools” include molecular analyses to verify species identities and likely geographic area of origin, climate matching and ecological niche modeling, preservation of natural enemy genetic diversity in quarantine, the use of theory from invasion biology to maximize establishment likelihoods for natural enemies, and improved understanding of the interactions between natural enemy and target pest microbiomes. This review suggests that opportunities exist for revisiting old pest problems and funding research programs using “new tools” for developing biological control programs for “legacy pests” could provide permanent suppression of some seemingly intractable pest problems. As a case study, we use citricola scale, Coccus pseudomagnoliarum, an invasive legacy pest of California citrus, to demonstrate the potential of new tools to support a new classical biological control program targeting this insect. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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Open AccessArticle

Efficacies and Second-Year Effects of SPLAT GM™ and SPLAT GM™ Organic Formulations

by Ksenia S. Onufrieva, Andrea D. Hickman, Donna S. Leonard and Patrick C. Tobin

Insects 2015, 6(1), 1-12; https://doi.org/10.3390/insects6010001 - 23 Dec 2014

Cited by 4 | Viewed by 6040

Abstract

Mating disruption is the primary control tactic used against the gypsy moth, Lymantria dispar (L.) (Lepidoptera: Lymantriidae) under the gypsy moth Slow the Spread (STS) program. In this paper, we present the results of the multiyear study designed to evaluate a new liquid [...] Read more.

Mating disruption is the primary control tactic used against the gypsy moth, Lymantria dispar (L.) (Lepidoptera: Lymantriidae) under the gypsy moth Slow the Spread (STS) program. In this paper, we present the results of the multiyear study designed to evaluate a new liquid SPLAT GM™ (ISCA Tech, Riverside, CA, USA) Organic formulation, which is approved by the USDA to meet National Organic Program Standards for use in organic certified farms, for its ability to disrupt gypsy moth mating, and to evaluate the environmental persistence of SPLAT GM™ and SPLAT GM™ Organic formulations. Environmental persistence of the pheromone beyond the year of application is a significant concern since STS relies on trap catch data to evaluate treatment success. The study was conducted in 2007–2012 in forested areas in Virginia and Wisconsin, USA. We observed that SPLAT GM™ Organic reduced gypsy moth trap catch by ≥90% for 10 weeks in a similar manner as SPLAT GM™ and Hercon Disrupt^® II (Hercon Environmental, Emigsville, PA, USA). Although we observed persistent effects in all products one year after application, the persistence observed in SPLAT GM™ and SPLAT GM™ Organic was significantly lower than that of Hercon Disrupt^® II plastic laminated flakes. Full article

(This article belongs to the Special Issue Integrated Pest Management)

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Insects, Volume 6, Issue 1 (March 2015) – 19 articles , Pages 1-296

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