Molecular Characteristics and Antibiotic Resistance of Staphylococcus aureus Isolated from Patient and Food Samples in Shijiazhuang, China
Abstract
:1. Introduction
2. Methods
2.1. Bacterial Isolates
2.2. Extraction DNA and Detection of Antibiotic Resistance Genes
2.3. Antimicrobial Susceptibility Test
2.4. MLST
2.5. Statistical Analysis
3. Results
3.1. Antibiotic Resistance Profiles of S. aureus
3.2. STs and CCs of S. aureus Isolates
3.3. STs in MRSA and MSSA Isolates
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antimicrobial Agents | Patient Samples (n = 86) Resistance Rate (%) | ||
---|---|---|---|
Overall (n = 86) | MRSA (n = 38) | MSSA (n = 48) | |
PEN | 84 (97.7%) | 38 (100) | 46 (95.8) |
FOX | 38 (44.2%) | 38 (100) ** | 0 (0) |
OXA | 37 (43.0%) | 37 (97.4) ** | 0 (0) |
TET | 34 (39.5%) | 26 (68.4) ** | 8 (16.7) |
DOX | 14 (16.3%) | 14 (36.8) ** | 0 (0) |
CAP | 3 (3.5%) | 1 (2.6) | 2 (4.2) |
RFP | 5 (5.8%) | 4 (10.5) | 1 (2.1) |
ERY | 65 (75.6%) | 28 (73.7) | 37 (77.1) |
CLR | 65 (75.6%) | 28 (73.7) | 37 (77.1) |
SMZ | 64 (74.4%) | 26 (68.4) | 38 (79.2) |
GM | 17 (19.8%) | 9 (23.7) | 8 (16.7) |
CLI | 53 (61.6%) | 28 (73.7) * | 25 (52.1) |
CIP | 25 (29.1%) | 21 (55.3) ** | 4 (8.3) |
LEV | 22 (25.6%) | 20 (52.6) ** | 2 (4.2) |
CCs | STs Number | Patient Samples | Food Samples | ||||
---|---|---|---|---|---|---|---|
Sputum | Pus and Secretion | Blood | Wound and Swabs | Others | Meats | ||
CC1 | ST1 | 0 | 0 | 0 | 0 | 0 | 4 |
ST9 | 0 | 0 | 0 | 0 | 0 | 2 | |
ST2315 | 0 | 0 | 0 | 1 | 0 | 0 | |
ST6769 | 1 | 0 | 0 | 0 | 0 | 0 | |
CC5 | ST5 | 14 | 0 | 2 | 3 | 2 | 0 |
ST6 | 0 | 0 | 0 | 0 | 1 | 2 | |
ST306 | 0 | 0 | 0 | 0 | 1 | 0 | |
CC8 | ST72 | 1 | 0 | 0 | 0 | 0 | 0 |
ST239 | 2 | 1 | 0 | 0 | 2 | 0 | |
CC15 | ST15 | 1 | 0 | 0 | 0 | 2 | 1 |
CC22 | ST22 | 2 | 0 | 0 | 3 | 5 | 0 |
CC121 | ST121 | 0 | 0 | 0 | 0 | 1 | 0 |
ST7 | ST7 | 2 | 0 | 0 | 0 | 0 | 2 |
ST20 | ST20 | 1 | 1 | 0 | 0 | 0 | 0 |
ST25 | ST25 | 0 | 1 | 0 | 0 | 0 | 0 |
ST59 | ST59 | 6 | 8 | 1 | 2 | 5 | 2 |
ST88 | ST88 | 0 | 0 | 0 | 0 | 1 | 0 |
ST398 | ST398 | 1 | 2 | 1 | 0 | 3 | 0 |
ST522 | ST522 | 0 | 0 | 0 | 0 | 0 | 1 |
ST1281 | ST1281 | 0 | 0 | 0 | 0 | 1 | 0 |
ST2472 | ST2472 | 0 | 0 | 0 | 0 | 0 | 1 |
ST3068 | ST3068 | 1 | 0 | 0 | 0 | 0 | 0 |
ST3355 | ST3355 | 1 | 0 | 0 | 0 | 0 | 0 |
NEW | NEW | 1 | 0 | 0 | 0 | 0 | 0 |
ST3069 | ST3069 | 0 | 0 | 0 | 1 | 0 | 0 |
ST4845 | ST4845 | 0 | 0 | 0 | 1 | 0 | 0 |
Total | 34 | 13 | 4 | 11 | 24 | 15 |
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Zhang, H.; Qin, L.; Jin, C.; Ju, H.; Jiang, R.; Li, L.; Zhang, H.; Gao, W.; Wei, X.; Dong, H.; et al. Molecular Characteristics and Antibiotic Resistance of Staphylococcus aureus Isolated from Patient and Food Samples in Shijiazhuang, China. Pathogens 2022, 11, 1333. https://doi.org/10.3390/pathogens11111333
Zhang H, Qin L, Jin C, Ju H, Jiang R, Li L, Zhang H, Gao W, Wei X, Dong H, et al. Molecular Characteristics and Antibiotic Resistance of Staphylococcus aureus Isolated from Patient and Food Samples in Shijiazhuang, China. Pathogens. 2022; 11(11):1333. https://doi.org/10.3390/pathogens11111333
Chicago/Turabian StyleZhang, Han, Liyun Qin, Caiping Jin, Huidong Ju, Ruiping Jiang, Lijie Li, Hong Zhang, Weili Gao, Xiuping Wei, Hongyan Dong, and et al. 2022. "Molecular Characteristics and Antibiotic Resistance of Staphylococcus aureus Isolated from Patient and Food Samples in Shijiazhuang, China" Pathogens 11, no. 11: 1333. https://doi.org/10.3390/pathogens11111333