Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey
Abstract
:1. Introduction
2. Results
2.1. Phytoplasma Detection and Symptom Observation
2.2. Sequence Typing by RFLP Analyses and Phytogenetic Relationships
3. Discussion
4. Material and Methods
4.1. Sources of Nucleic Acids and Field Sampling
4.2. Isolation of Nucleic Acids and PCR Detection of Phytoplasmas
4.3. Molecular Characterization of the Strains Based on Tuf, VMP1, and SecY Genes
4.4. Sequencing and Phylogenetic Analyses
4.5. In Silico RFLP Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Çağlar, B.K.; Şimşek, E. Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey. Pathogens 2022, 11, 1031. https://doi.org/10.3390/pathogens11091031
Çağlar BK, Şimşek E. Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey. Pathogens. 2022; 11(9):1031. https://doi.org/10.3390/pathogens11091031
Chicago/Turabian StyleÇağlar, Behçet Kemal, and Eray Şimşek. 2022. "Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey" Pathogens 11, no. 9: 1031. https://doi.org/10.3390/pathogens11091031