Impact of Sclerotinia sclerotiorum Infection on Lettuce (Lactuca sativa L.) Survival and Phenolics Content—A Case Study in a Horticulture Farm in Poland
Abstract
:1. Introduction
2. Materials and Methods
2.1. Lettuce Cultivars, Planting, and Cultivation
2.1.1. The Summer (1st) Crop, 2016
2.1.2. The Autumn (2nd) Crop, 2016
2.2. Air and Soil Parameters
2.3. S. sclerotiorum Soil Contamination and Re-Isolation of the Fungus
2.4. Evaluation of Lettuce Infection
2.5. Analyses of Phenolics Contents
2.5.1. Sample Collection and Preparation of Plant Extracts
2.5.2. Total Phenolic Compounds (TPCs)
2.5.3. Flavonoids
2.5.4. Anthocyanins
2.6. Statistical Analysis
3. Results
3.1. Changes in the Air and Soil Parameters
3.2. Re-Isolation of the Pathogen
3.3. Evaluation of Disease Progression and Lettuce Survival
3.4. Analysis of Phenolics Content
3.4.1. Total Content of Phenolic Compounds (TPCs)
3.4.2. Flavonoid Content
3.4.3. Anthocyanin Content
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
References
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Stage of Infection | Presence of the Fungus | Symptom on Plant |
---|---|---|
0 | No fungus | No symptoms |
1 | Small white mycelium | Small necroses on the stem |
2 | White mycelium and black sclerotia on the stem | Wilted leaf base, necroses on leaves and stem |
3 | White mycelium and sclerotia on leaves and stems | Necroses on leaves and stems, marginal leaves wilted and brown |
4 | Mycelium spread and many black sclerotia | Wilted, rotten, and discolored leaves, rotten stem |
5 | Spreading fungus on the whole plant | Decoyed, dead plant |
Cultivation Week | I | II | III | IV | V | VI | VII |
---|---|---|---|---|---|---|---|
Cultivar | Plants in control field (%) | ||||||
Crispy iceberg Templin | 98 | 92 1 | 90 1 | 82 1 | 80 1 | 80 1 | 80 1 |
Leafy red Lollo Rossa | 100 | 100 | 99 | 99 | 99 | 99 | 99 |
Leafy green Lollo Bionda | 99 | 99 | 93 | 92 | 92 | 92 | 92 |
Plants in infected field (%) | |||||||
Crispy iceberg Templin | 100 | 97 | 95 | 80 | 70 | 21 | 10 |
Leafy red Lollo Rossa | 97 | 96 | 90 | 89 | 89 | 84 | 83 |
Leafy green Lollo Bionda | 100 | 94 | 93 | 93 | 79 | 70 | 70 |
Cultivation Week | I | II | III | IV | V | VI | VII |
---|---|---|---|---|---|---|---|
Cultivar | Plants in control field (%) | ||||||
Crispy iceberg Templin | 100 | 100 | 100 | 98 | 96 | 96 | 92 |
Leafy red Lollo Rossa | 100 | 100 | 100 | 100 | 99 | 99 | 97 |
Leafy green Lollo Bionda | 100 | 100 | 100 | 99 | 99 | 99 | 97 |
Plants in infected field (%) | |||||||
Crispy iceberg Templin | 100 | 97 | 94 | 70 | 52 | 48 | 30 |
Leafy red Lollo Rossa | 98 | 88 | 81 | 80 | 76 | 65 | 51 |
Leafy green Lollo Bionda | 100 | 99 | 98 | 88 | 85 | 80 | 60 |
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Macioszek, V.K.; Marciniak, P.; Kononowicz, A.K. Impact of Sclerotinia sclerotiorum Infection on Lettuce (Lactuca sativa L.) Survival and Phenolics Content—A Case Study in a Horticulture Farm in Poland. Pathogens 2023, 12, 1416. https://doi.org/10.3390/pathogens12121416
Macioszek VK, Marciniak P, Kononowicz AK. Impact of Sclerotinia sclerotiorum Infection on Lettuce (Lactuca sativa L.) Survival and Phenolics Content—A Case Study in a Horticulture Farm in Poland. Pathogens. 2023; 12(12):1416. https://doi.org/10.3390/pathogens12121416
Chicago/Turabian StyleMacioszek, Violetta Katarzyna, Paulina Marciniak, and Andrzej Kiejstut Kononowicz. 2023. "Impact of Sclerotinia sclerotiorum Infection on Lettuce (Lactuca sativa L.) Survival and Phenolics Content—A Case Study in a Horticulture Farm in Poland" Pathogens 12, no. 12: 1416. https://doi.org/10.3390/pathogens12121416