SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation
Abstract
:1. Introduction
2. Results
2.1. SseL Reduces the Nuclear Abundance of RPS3
2.2. SseL Binds to RPS3
2.3. SseL Deubiquitinates RPS3
2.4. SseL DUB Activity Is Important to Inhibiting RPS3 Nuclear Translocation
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Conflicts of Interest
References
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Strain/Plasmid | Description | Source |
---|---|---|
Strain | ||
E. coli BL21(DE3) | E. coli F−ompT hsdSB (rB−_m B−) gal dcm (DE3) | Novagen |
BL21(DE3)/NleH1-pET42a | GST-EHEC NleH1 | [19] |
BL21(DE3)/SseL-pET42a | GST-S. Typhimurium SseL | This study |
BL21(DE3)/RPS3-pET28a | His-RPS3 | This study |
BL21(DE3)NleB1-pET42a | GST-EHEC NleB1 | [23] |
BL21(DE3)/SseL(C262A)-pET42a | GST-S. Typhimurium SseL(C262A) | This study |
Plasmid | ||
HA | HA fusion expression | Clontech |
NleH1-HA | HA fused to E. coli EDL933 NleH1 | [19] |
SseL-HA | HA fused to S. Typhimurium SseL | This study |
SseL(C262A)-HA | HA fused to S. Typhimurium SseL(C262A) | This study |
FliC-HA | HA fused to ETEC FliC | [39] |
3× FLAG | FLAG expression | Sigma |
3× FLAG-RPS3 | FLAG-RPS3 | [16] |
pET42a | Bacterial GST fusion expression | Novagen |
NleH1-pET42a | GST-EHEC NleH1 | [19] |
NleB1-pET42a | GST-EHEC NleB1 | [23] |
SseL-pET42a | GST-S. Typhimurium SseL | This study |
SseL(C262A)-pET42a | GST-S. Typhimurium SseL(C262A) | This study |
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Wu, M.; El Qaidi, S.; Hardwidge, P.R. SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation. Pathogens 2018, 7, 86. https://doi.org/10.3390/pathogens7040086
Wu M, El Qaidi S, Hardwidge PR. SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation. Pathogens. 2018; 7(4):86. https://doi.org/10.3390/pathogens7040086
Chicago/Turabian StyleWu, Miaomiao, Samir El Qaidi, and Philip R. Hardwidge. 2018. "SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation" Pathogens 7, no. 4: 86. https://doi.org/10.3390/pathogens7040086