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Pathogens
Volume 8
Issue 4
10.3390/pathogens8040237
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Article

Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital

by
Kathy Montes
1,
Bryan Ortiz
1,
Celeste Galindo
1,2,
Isis Figueroa
1,
Sharleen Braham
3 and
Gustavo Fontecha
1,*
1
Microbiology Research Institute, Universidad Nacional Autónoma de Honduras, Tegucigalpa 11101, Honduras
2
Instituto Hondureño de Seguridad Social, Tegucigalpa 11101, Honduras
3
Microbiology Research Institute, Universidad Nacional Autonoma de Honduras, Tegucigalpa 11101, Honduras
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 237; https://doi.org/10.3390/pathogens8040237
Submission received: 16 September 2019 / Revised: 25 October 2019 / Accepted: 27 October 2019 / Published: 15 November 2019
(This article belongs to the Section Human Pathogens)
Review Reports Versions Notes

Abstract

Candida species are one of the most important causes of human infections, especially in hospitals and among immunocompromised patients. The correct and rapid etiological identification of yeast infections is important to provide adequate therapy, reduce mortality, and control outbreaks. In this study, Candida species were identified in patients with suspected fungal infection, and phenotypic and genotypic identification methods were compared. A total of 167 axenic fungal cultures and 46 clinical samples were analyzed by HardyCHROM®, MicroScan®(Omron Microscan Systems Inc, Renton, WA, USA), and PCR-RFLP (Restriction Fragment Length Polymorphisms). The species of the C. albicans complex were the most frequent, followed by C. tropicalis and C. glabrata. Less common but clinically relevant species of Candida were also isolated. The comparison between the three methods was concordant, especially for the most common Candida species. Fungal DNA amplification was successful in all clinical samples.
Keywords: Candida spp.; PCR-RFLP; Honduras; HardyCHROM®; MicroScan® Candida spp.; PCR-RFLP; Honduras; HardyCHROM®; MicroScan®

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MDPI and ACS Style

Montes, K.; Ortiz, B.; Galindo, C.; Figueroa, I.; Braham, S.; Fontecha, G. Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital. Pathogens 2019, 8, 237. https://doi.org/10.3390/pathogens8040237

AMA Style

Montes K, Ortiz B, Galindo C, Figueroa I, Braham S, Fontecha G. Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital. Pathogens. 2019; 8(4):237. https://doi.org/10.3390/pathogens8040237

Chicago/Turabian Style

Montes, Kathy, Bryan Ortiz, Celeste Galindo, Isis Figueroa, Sharleen Braham, and Gustavo Fontecha. 2019. "Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital" Pathogens 8, no. 4: 237. https://doi.org/10.3390/pathogens8040237

APA Style

Montes, K., Ortiz, B., Galindo, C., Figueroa, I., Braham, S., & Fontecha, G. (2019). Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital. Pathogens, 8(4), 237. https://doi.org/10.3390/pathogens8040237

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