Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection
Abstract
:1. Introduction
2. Results
2.1. Cell Viability
2.2. Detection of CAV Antigens
2.3. Detection of the CAV Genome within Infected Cells and Cell Supernatants
2.4. Recovery of CAV during Passage
2.5. Genetic Analyses of Virus Recovered from Passaged Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture, Virus, Titration, and Virus Isolation
4.2. Antiserum, Monoclonal Antibodies, and Anti-Peptide Antibodies
4.3. Detection of Cell Viability
4.4. DNA Extraction and Real-Time PCR
4.5. IFAT
4.6. Experimental Design for In Vitro Persistent Infection
4.7. Nucleotide Sequencing and Genetic Analyses
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | CAV Isolation (TCID50/106 cells) | |||
---|---|---|---|---|
No. of Passages | ||||
P1 | P5 | P10 | P14 | |
cells | 5.32 | 6.04 | 5.9 | 6.28 |
sup | <0.7 | <0.7 | <0.7 | <0.7 |
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Van Dong, H.; Tran, G.T.H.; Trinh, D.Q.; Takeda, Y.; Ogawa, H.; Imai, K. Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection. Pathogens 2020, 9, 842. https://doi.org/10.3390/pathogens9100842
Van Dong H, Tran GTH, Trinh DQ, Takeda Y, Ogawa H, Imai K. Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection. Pathogens. 2020; 9(10):842. https://doi.org/10.3390/pathogens9100842
Chicago/Turabian StyleVan Dong, Hieu, Giang Thi Huong Tran, Dai Quang Trinh, Yohei Takeda, Haruko Ogawa, and Kunitoshi Imai. 2020. "Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection" Pathogens 9, no. 10: 842. https://doi.org/10.3390/pathogens9100842