Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland
Abstract
:1. Introduction
2. Results
2.1. Field Observations
2.2. Identificaiton and Characteristics of Bacterial Strains
2.3. Pathogenicity Assessment of Strains
3. Discussion
4. Materials and Methods
4.1. Plant and Insect Material
4.2. Bacteria Isolation from Plants
4.3. Bacteria Isolation from Insects
4.4. Bacterial Identification, DNA Extraction, and Sequence Analysis
4.5. Phylogenetic Analysis of the Tested Pantoea Ananatis Strains
4.6. Assessing Pathogenicity of Bacterial Strains
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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No | Strain Tested | Symptoms Group 1/Season | Biolog Gen III Identification Result | Wheat Pathogenic (Yes/No) | 16S rDNA Sequencing Result (GenBank Accession Number) | gyrB Sequencing Result (GenBank Accession Number) |
---|---|---|---|---|---|---|
Season 2018 | ||||||
1 | Ta015 | 1/2018 | Bacillus licheniformis | No | nt | nt |
2 | Ta016 | 1/2018 | Bacillus licheniformis | No | nt | nt |
3 | Ta017 | 1/2018 | Pantoea agglomerans | No | nt | nt |
4 | Ta018 | 1/2018 | Bacillus licheniformis | No | nt | nt |
5 | Ta019 | 1/2018 | Macrococcus brunensis | No | nt | nt |
6 | Ta020 | 1/2018 | Macrococcus brunensis | No | nt | nt |
7 | Ta021 | 1/2018 | Pantoea agglomerans | No | nt | nt |
8 | Ta022 | 1/2018 | Pantoea agglomerans | No | nt | nt |
9 | Ta023 | 2/2018 | Bacillus licheniformis | No | nt | nt |
10 | Ta024 | 3/2018 | Pantoea ananatis | Yes | MH973236 | MT091018 |
11 | Ta025 | 3/2018 | Bacillus licheniformis | No | nt | nt |
12 | Ta026 | 3/2018 | Pseudomonas fluorescens | No | nt | nt |
13 | Ta027 | 3/2018 | Pantoea ananatis | Yes | MH973237 | MT091019 |
14 | Ta028 | 2/2018 | Pantoea agglomerans | No | nt | nt |
15 | Ta029 | 3/2018 | Pseudomonas fluorescens | No | nt | nt |
16 | Ta030 | 3/2018 | Pantoea ananatis | Yes | MH973238 | MT091020 |
17 | Ta031 | 2/2018 | Bacillus licheniformis | No | nt | nt |
18 | Ta032 | 2/2018 | Macrococcus brunensis | No | nt | nt |
19 | Ta033 | 2/2018 | Bacillus pseudomycoides | No | nt | nt |
20 | Ta034 | 2/2018 | Pseudomonas flavescens | No | nt | nt |
21 | Ta035 | 2/2018 | Pseudomonas flavescens | No | nt | nt |
22 | Ta036 | 2/2018 | Pantoea agglomerans | No | nt | nt |
Season 2019 | ||||||
23 | Ta037 | 2/2019 | Pantoea agglomerans | No | nt | nt |
24 | Ta038 | 2/2019 | Pantoea agglomerans | No | nt | nt |
25 | Ta039 | 2/2019 | Pseudomonas synthaxa | No | nt | nt |
26 | Ta040 | 2/2019 | Pseudomonas synthaxa | No | nt | nt |
27 | Ta041 | 1/2019 | Pantoea agglomerans | No | nt | nt |
28 | Ta042 | 1/2019 | Pantoea agglomerans | No | nt | nt |
29 | Ta043 | 1/2019 | Pantoea agglomerans | No | nt | nt |
30 | Ta044 | 1/2019 | Pantoea agglomerans | No | nt | nt |
31 | Ta045 | 3/2019 | Pseudomonas fluorescens | No | nt | nt |
32 | Ta046 | 3/2019 | Pantoea ananatis | Yes | MH973239 | MT091021 |
No | Strain Tested | Sample/Season | Biolog Gen III identification Result | Wheat Pathogenic (Yes/No) | 16S rDNA Sequencing Result (GenBank Accession Number) | gyrB Sequencing Result (GenBank Accession Number) |
---|---|---|---|---|---|---|
Season 2018 | ||||||
1 | OUC1 | 1/2018 | Pantoea ananatis | Yes | MT234395 | MT091022 |
2 | OUC2 | 1/2018 | Pantoea agglomerans | No | nt | nt |
3 | OUC3 | 1/2018 | Serratia liquefaciens | No | nt | nt |
4 | OUD1 | 2/2018 | Pantoea agglomerans | No | nt | nt |
5 | OUD2 | 2/2018 | Pantoea ananatis | Yes | MT234396 | MT091023 |
6 | OUD3 | 2/2018 | Serratia marcescens | No | nt | nt |
7 | OUE1 | 3/2018 | No ID | No | nt | nt |
8 | OUE2 | 3/2018 | No ID | No | nt | nt |
9 | OUE3 | 3/2018 | No ID | No | nt | nt |
Season 2019 | ||||||
10 | OUF1 | 1/2019 | Pantoea agglomerans | No | nt | nt |
11 | OUF2 | 1/2019 | Pantoea ananatis | Yes | MT234397 | MT091024 |
12 | OUF3 | 1/2019 | Acinetobacter johnstonii | No | nt | nt |
13 | OUG1 | 2/2019 | Pantoea ananatis | Yes | MT234398 | MT091025 |
14 | OUG2 | 2/2019 | Serratia marcescens | No | nt | nt |
15 | OUG3 | 2/2019 | Enterobacter aerogenes | No | nt | nt |
16 | OUH1 | 3/2019 | Acinetobacter johnstonii | No | nt | nt |
17 | OUH2 | 3/2019 | No ID | No | nt | nt |
18 | OUH3 | 3/2019 | No ID | No | nt | nt |
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Krawczyk, K.; Wielkopolan, B.; Obrępalska-Stęplowska, A. Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland. Pathogens 2020, 9, 1079. https://doi.org/10.3390/pathogens9121079
Krawczyk K, Wielkopolan B, Obrępalska-Stęplowska A. Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland. Pathogens. 2020; 9(12):1079. https://doi.org/10.3390/pathogens9121079
Chicago/Turabian StyleKrawczyk, Krzysztof, Beata Wielkopolan, and Aleksandra Obrępalska-Stęplowska. 2020. "Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland" Pathogens 9, no. 12: 1079. https://doi.org/10.3390/pathogens9121079
APA StyleKrawczyk, K., Wielkopolan, B., & Obrępalska-Stęplowska, A. (2020). Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland. Pathogens, 9(12), 1079. https://doi.org/10.3390/pathogens9121079