Increasing the Safety Profile of the Master Donor Live Attenuated Influenza Vaccine
Abstract
:1. Introduction
2. Results
2.1. The MDV A/AA/6/60 LAIV PA-X and NS1 Proteins Inhibit Host Gene Expression
2.2. Generation of Recombinant WT and Mutant MDV A/AA/60 LAIVs Containing Modified PA and/or NS
2.3. Growth Kinetics of Recombinant WT and Mutant MDV A/AA/6/60 LAIVs
2.4. In Vivo Safety of MDV A/AA/6/60 LAIVs with Varying Ability to Inhibit Host Gene Expression
2.5. Induction of Humoral Immune Responses by WT and Mutant MDV A/AA/6/60 LAIVs
2.6. Protective Efficacy of MDV A/AA/6/60 LAIVs Against a Homologous pH1N1 Viral Challenge
3. Discussion
4. Materials and Methods
4.1. Cells and Viruses
4.2. Plasmids
4.3. Viral Rescues
4.4. Inhibition of Host Gene Expression
4.5. SDS-PAGE and Western Blot Analysis
4.6. Viral Growth Kinetics
4.7. Plaque Assay and Immunostaining
4.8. Mouse Experiments
4.9. Enzyme-Linked Immunosorbent Assay (ELISA)
4.10. Hemagglutination inhibition (HAI) Assay
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hilimire, T.A.; Nogales, A.; Chiem, K.; Ortego, J.; Martinez-Sobrido, L. Increasing the Safety Profile of the Master Donor Live Attenuated Influenza Vaccine. Pathogens 2020, 9, 86. https://doi.org/10.3390/pathogens9020086
Hilimire TA, Nogales A, Chiem K, Ortego J, Martinez-Sobrido L. Increasing the Safety Profile of the Master Donor Live Attenuated Influenza Vaccine. Pathogens. 2020; 9(2):86. https://doi.org/10.3390/pathogens9020086
Chicago/Turabian StyleHilimire, Thomas A., Aitor Nogales, Kevin Chiem, Javier Ortego, and Luis Martinez-Sobrido. 2020. "Increasing the Safety Profile of the Master Donor Live Attenuated Influenza Vaccine" Pathogens 9, no. 2: 86. https://doi.org/10.3390/pathogens9020086