Hepatic Stellate Cells and Hepatocytes as Liver Antigen-Presenting Cells during B. abortus Infection
Abstract
:1. Introduction
2. Results
2.1. B. abortus Infection Induces MHC-I and -II Expression in LX-2 Cells via a T4SS-Independent Mechanism
2.2. B. abortus Infection Induce CIITA and Cathepsin S Transcription in LX-2 cells
2.3. B. abortus Infection Does not Induce the Expression of Costimulatory Molecules CD80, CD86 and CD40
2.4. B. abortus Increase the Phagocytic Capability of LX-2 Cells
2.5. B. abortus Induces MHC-II-Restricted Antigen Processing and Presentation by LX-2 Cells
2.6. Culture Supernatants from B. abortus Infected THP-I Cells Do not Induce MHC-I and MHC-II Expression by LX2 Cells
2.7. Culture Supernatants from B. abortus Infected THP-I Monocytes Inhibit MHC-II Expression Induced by IFN-γ in an IL-10 Dependent Mechanism.
2.8. B. abortus Infection Induces MHC-I Expression in HepG2 Cells but Does not Alter MHC-II Levels
3. Discussion
4. Materials and Methods
4.1. Bacterial Culture
4.2. Cell Culture
4.3. Cellular Infection
4.4. Flow Cytometry
4.5. Cytokine ELISA
4.6. Phagocytosis Assays
4.7. mRNA Preparation and RT-qPCR
4.8. Ag Processing and Presentation Assays
4.9. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Arriola Benitez, P.C.; Pesce Viglietti, A.I.; Elizalde, M.M.; Giambartolomei, G.H.; Quarleri, J.F.; Delpino, M.V. Hepatic Stellate Cells and Hepatocytes as Liver Antigen-Presenting Cells during B. abortus Infection. Pathogens 2020, 9, 527. https://doi.org/10.3390/pathogens9070527
Arriola Benitez PC, Pesce Viglietti AI, Elizalde MM, Giambartolomei GH, Quarleri JF, Delpino MV. Hepatic Stellate Cells and Hepatocytes as Liver Antigen-Presenting Cells during B. abortus Infection. Pathogens. 2020; 9(7):527. https://doi.org/10.3390/pathogens9070527
Chicago/Turabian StyleArriola Benitez, Paula Constanza, Ayelén Ivana Pesce Viglietti, María Mercedes Elizalde, Guillermo Hernán Giambartolomei, Jorge Fabián Quarleri, and María Victoria Delpino. 2020. "Hepatic Stellate Cells and Hepatocytes as Liver Antigen-Presenting Cells during B. abortus Infection" Pathogens 9, no. 7: 527. https://doi.org/10.3390/pathogens9070527