Pichinde Virus Infection of Outbred Hartley Guinea Pigs as a Surrogate Animal Model for Human Lassa Fever: Histopathological and Immunohistochemical Analyses
Abstract
:1. Introduction
2. Results
2.1. Overview of the Viral Strains Used and the Disease Symptoms Produced by Them
2.2. Histopathological and Immunohistochemical Analyses of Tissue Sections Prepared from Representative Healthy Guinea Pigs after Injection with Either PBS or the Parental P2 PICV
2.3. Histopathological and Immunohistochemical Analyses of Tissue Sections Prepared from a Representative Moribund Guinea Pig Due to the Parental P18 PICV Infection
2.4. Histopathological and Immunohistochemical Analyses of Tissue Sections Prepared from a Representative Moribund Guinea Pig Due to the Recombinant rP18 PICV Infection
2.5. Histopathological and Immunohistochemical Analyses of Tissue Sections Prepared from a Representative Guinea Pig Infected with a Recombinant rS2L18 Reassortant Virus
2.6. Histopathological and Immunohistochemical Analyses of Tissue Sections Prepared from a Representative Guinea Pig Infected with a Recombinant rS18L2 Reassortant Virus
3. Discussion
4. Materials and Methods
4.1. In Vivo Experiment
4.2. Histopathological and Immunohistochemical Analyses
4.3. PICV Plaque Assay
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Guinea Pig ID | Viruses | Fever, BW Loss 1 | DoE 2 | Health, BW at DoE 3 |
---|---|---|---|---|
867508 | PBS | No fever, no BW loss | 18 dpi | Healthy, BW 139% |
867474 | P2 | Fever d6 to d9, BW loss d9 to d10 | 18 dpi | Healthy, BW 132% |
867480 | P18 | Fever d5 to d13, BW loss since d8 | 14 dpi | Moribund, BW 74% |
867488 | rP18 | Fever d5 to d17 BW loss since d9 | 18 dpi | Moribund BW 80% |
867494 | rS2L18 | Fever d6 to d10 BW loss d9 to d10 | 18 dpi | Healthy, BW 116% |
867500 | rS18L2 | Fever d6 to d13 BW loss d9 to d16 | 18 dpi | Healthy, BW 102% |
Guinea Pig ID | Viruses | 6 dpi (PFU/mL) | 12 dpi (PFU/mL) | TP 1 or 18 dpi (PFU/mL) |
---|---|---|---|---|
867474 | P2 | BD | BD | BD |
867480 | P18 | 360 | 3.4 × 104 | 4 × 106 |
867488 | rP18 | NA | NA | 1.8 × 106 |
867494 | rS2L18 | BD | BD | BD |
867500 | rS18L2 | BD | BD | BD |
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Shieh, W.-J.; Lan, S.; Zaki, S.R.; Ly, H.; Liang, Y. Pichinde Virus Infection of Outbred Hartley Guinea Pigs as a Surrogate Animal Model for Human Lassa Fever: Histopathological and Immunohistochemical Analyses. Pathogens 2020, 9, 579. https://doi.org/10.3390/pathogens9070579
Shieh W-J, Lan S, Zaki SR, Ly H, Liang Y. Pichinde Virus Infection of Outbred Hartley Guinea Pigs as a Surrogate Animal Model for Human Lassa Fever: Histopathological and Immunohistochemical Analyses. Pathogens. 2020; 9(7):579. https://doi.org/10.3390/pathogens9070579
Chicago/Turabian StyleShieh, Wun-Ju, Shuiyun Lan, Sherif R. Zaki, Hinh Ly, and Yuying Liang. 2020. "Pichinde Virus Infection of Outbred Hartley Guinea Pigs as a Surrogate Animal Model for Human Lassa Fever: Histopathological and Immunohistochemical Analyses" Pathogens 9, no. 7: 579. https://doi.org/10.3390/pathogens9070579