Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm
Abstract
:1. Introduction
2. Results
2.1. Diversity and Isolation Frequency of Streptomyces Species from Different Regions
2.2. Phenotypic Characterization
2.3. Pathogenicity Tests/Virulence Confirmation Assays
2.4. Screening of Potato Germplasm against S. scabies
2.5. Measurement of the Percentage of Potato Scab Incidence
2.6. Measurement of Potato Scab Index and Percent Disease Severity
2.7. Effect of Streptomyces scabies on Growth Parameters of Potato Cultivars
2.8. Molecular Detection of Streptomyces scabies (Gel Electrophoresis and PCR Amplification)
2.9. Sequence Analysis
3. Discussion
4. Materials and Methods
4.1. Diversity and Isolation of Streptomyces Species from Different Regions
4.2. Phenotypic Characterization
4.3. Pathogenicity Tests/Virulence Confirmation Assays
4.4. Screening of Potato Germplasm against Streptomyces scabies
4.5. Percentage of Disease Incidence
4.6. Percentage of Potato Scab Index and Disease Severity
- 0 = No symptom,
- 1 = Very small lesions,
- 2 = Small superficial lesions,
- 3 = Periderm broken,
- 4 = Light pitted
- 5 = Deep pitted
4.7. Effect of Streptomyces scabies on Growth Parameters of Potato Cultivars
4.8. Molecular Detection of S. scabies (Gel Electrophoresis and PCR Amplification)
4.9. Sequence Analysis
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Area | Location | Disease Incidence (%) | Percent Scab Index |
---|---|---|---|
Taxila (Rawalpindi) | Thatha | 2% | 3.1% |
Usman Khattir | 3% | 3.2% | |
Ghari sikandar | 16% | 6.7% | |
Khurum Paracha | 12% | 5.5% | |
Labthatoo | 5% | 3.6% | |
Average | 8% | 4.42% | |
Hazro (Attock) | Waisa | 13% | 9.6% |
Mallah | 3% | 2.6% | |
Shamsabad | 11% | 8.2% | |
Sirka | 2% | 1.9% | |
Shadi khan | 9% | 4.6% | |
Kalu kalan | 5% | 2.5% | |
Average | 7% | 4.90% | |
Sialkot | Kishan garh | 22% | 13.7% |
Hussain pur | 23% | 13.9% | |
Randhir | 34% | 18.7% | |
Habibpur | 41% | 21.2% | |
Chicharwali | 21% | 13.2% | |
Average | 28% | 16.14% | |
Kasur | Qutba | 40% | 22.6% |
Faqeerwala | 38% | 21.8% | |
Dolaywala | 42% | 23.1% | |
Average | 40% | 22.5% | |
Okara | Moza Ameer | 36% | 23.8% |
Salwal | 45% | 17.5% | |
Qadirabad | 52% | 25.2% | |
Average | 44.33% | 22.16% | |
Faisalabad | Jaranwala | 32% | 15.1% |
Ram Diwali | 26% | 14.9% | |
Karamsar | 24% | 18.7% | |
Average | 27.33% | 16.23% |
Isolates | Potato Cultivars | Location of Sampling | Suggested Identification |
---|---|---|---|
CS-TAX01 | Cardinal | Taxila (Rawalpindi) | Streptomyces scabies |
CS-TAX02 | Cardinal | Taxila (Rawalpindi) | Streptomyces acidiscabies |
CS-TAX002 | Asterix | Taxila (Rawalpindi) | Streptomyces scabies |
CS-TAX004 | Lady Rosetta | Taxila (Rawalpindi) | Streptomyces scabies |
CS-TAX06 | Lady Rosetta | Taxila (Rawalpindi) | Streptomyces griseoflavus |
CS-TAX08 | Faisalabad red | Taxila (Rawalpindi) | Streptomyces scabies |
CS-TAX010 | Faisalabad red | Taxila (Rawalpindi) | Streptomyces griseoflavus |
CS-TAX12 | Faisalabad red | Taxila (Rawalpindi) | Streptomyces griseoflavus |
CS-TAX13 | Faisalabad white | Taxila (Rawalpindi) | Streptomyces scabies |
CS-HAZ14 | Faisalabad white | Hazro (Attock) | Streptomyces scabies |
CS-HAZ15 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ18 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ20 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ24 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ40 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ56 | Sadaf | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ58 | Tourag | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ59 | Tourag | Hazro (Attock) | Streptomyces acidiscabies |
CS-HAZ62 | Tourag | Hazro (Attock) | Streptomyces scabies |
CS-SIAL060 | Tourag | Sialkot | Streptomyces scabies |
CS-SIAL70 | Diamant | Sialkot | Streptomyces scabies |
CS-SIAL72 | Diamant | Sialkot | Streptomyces scabies |
CS-SIAL74 | Diamant | Sialkot | Streptomyces scabies |
CS-SIAL76 | Diamant | Sialkot | Streptomyces scabies |
CS-SIAL78 | Diamant | Sialkot | Streptomyces griseoflavus |
CS-SIAL80 | Diamant | Sialkot | Streptomyces griseoflavus |
CS-SIAL90 | Diamant | Sialkot | Streptomyces griseoflavus |
CS-SIAL101 | Kuroda | Sialkot | Streptomyces griseoflavus |
CS-SIAL110 | Kuroda | Sialkot | Streptomyces griseoflavus |
CS-OK108 | Kuroda | Okara | Streptomyces griseoflavus |
CS-OK0101 | Kuroda | Okara | Streptomyces scabies |
CS-OK120 | Kuroda | Okara | Streptomyces scabies |
CS-OK124 | Asterix | Okara | Streptomyces scabies |
CS-OK126 | Asterix | Okara | Streptomyces scabies |
CS-OK128 | Asterix | Okara | Streptomyces scabies |
CS-OK130 | Faisalabad red | Okara | Streptomyces scabies |
CS-OK143 | Faisalabad white | Okara | Streptomyces griseoflavus |
CS-OK131 | Faisalabad white | Okara | Streptomyces griseoflavus |
CS-OK121 | Diamant | Okara | Streptomyces griseoflavus |
CS-FSD124 | Sadaf | Faisalabad | Streptomyces griseoflavus |
CS-FSD126 | Sadaf | Faisalabad | Streptomyces acidiscabies |
CS-FSD123 | Diamant | Faisalabad | Streptomyces acidiscabies |
CS-FSD128 | Kuroda | Faisalabad | Streptomyces acidiscabies |
CS-FSD129 | Lady Rosetta | Faisalabad | Streptomyces acidiscabies |
CS-FSD130 | Lady Rosetta | Faisalabad | Streptomyces acidiscabies |
CS-FSD139 | Sante | Faisalabad | Streptomyces scabies |
CS-FSD149 | Sante | Faisalabad | Streptomyces scabies |
CS-FSD0149 | Sante | Faisalabad | Streptomyces scabies |
CS-FSD143 | Diamant | Faisalabad | Streptomyces scabies |
CS-FSD146 | Diamant | Faisalabad | Streptomyces scabies |
Culture Media | Growth | Colony Color | Aerial Mycelium |
---|---|---|---|
nutrient agar | Moderate | Creamy White | Abundant |
Potato dextrose agar | Moderate | Creamy White | Abundant |
Yeast malt agar | Well Abundant | Creamy White | Well Abundant |
King’s B media | Moderate | White to Creamy | Poor |
Oat meal agar | Moderate | White | Abundant |
Potato yeast extract | Moderate | Brown | Abundant |
Czapek media | Moderate | White | Abundant |
Isolates | Spore Shape | Spore Color | Septations | Spore Size (μm) | |
---|---|---|---|---|---|
Length (Min–Max) Mean | Width(Min–Max) Mean | ||||
CS-TAX01 | Pointed from one end and blunted from another end | Hyaline | 2 celled actinomycetes conidia | (9–18) 13.5 | (5–6) 5.5 |
CS-TAX002 | Pointed from one end and blunted from other end | Hyaline | 2 celled actinomycetes conidia | (9–15) 12 | (5–6) 5.5 |
CS-TAX004 | Pointed from one end | Hyaline | 2 celled actinomycetes conidia | (9–20) 14.5 | (6–7) 6.5 |
CS-TAX08 | Pointed from one end | Hyaline | 2 celled actinomycetes conidia | (9–17) 13 | (5–7) 6 |
CS-TAX13 | Pointed from one end and blunted from other end | Hyaline | 2 celled actinomycetes conidia | (9–20) 14.5 | (6–7) 6.5 |
CS-HAZ14 | Pointed from one end | Hyaline | 2 celled actinomycetes conidia | (10–20) 15 | (6–7) 6.5 |
CS-HAZ62 | Pointed from one end | Hyaline | 2 celled actinomycetes conidia | (10–21) 15.5 | (5–7) 6 |
CS-SIAL060 | Pointed from one end | Hyaline | Single-cell actinomycetes conidia | (8–22) 15 | (3–7) 5 |
CS-SIAL70 | Pointed from one end and blunted from other end | Falcate | Single-cell actinomycetes conidia | (13–25) 14 | (4–7) 5.5 |
CS-SIAL72 | Pointed from one end and blunted from other end | Fusiform | Single-cell actinomycetes conidia | (12–22) 17 | (6–8) 7 |
CS-SIAL74 | Pointed from two ends | Falcate | 2 celled actinomycetes conidia | (15–21) 18 | (2–9) 5.5 |
CS-SIAL76 | Pointed from two ends | Hyaline | 2 celled actinomycetes conidia | (13–17) 15 | (5–8) 6.5 |
CS-OK0101 | Pointed from two ends | Hyaline | Single-cell actinomycetes conidia | (13–21) 17 | (5–10) 7.5 |
CS-OK120 | Pointed from one end and blunted from other end | Hyaline | Single-cell actinomycetes conidia | (16–17) 16.5 | (4–7) 5.5 |
CS-OK124 | Pointed from one end and blunted from other end | Falcate | Single-cell actinomycetes conidia | (15–25) 20 | (5–9) 7 |
CS-OK126 | Pointed from two ends | Falcate | Single-cell actinomycetes conidia | (12–19) 15.5 | (6–7) 6.5 |
CS-OK128 | Pointed from two ends | Falcate | 2 celled actinomycetes conidia | (15–22) 18.5 | (3–6) 4.5 |
CS-OK130 | Pointed from two ends | Hyaline | 2 celled actinomycetes conidia | (14–23) 13.5 | (2–7) 4.5 |
CS-FSD139 | Pointed from one end and blunted from other end | Hyaline | 2 celled actinomycetes conidia | (16–25) 20.5 | (3–10) 6.5 |
CS-FSD149 | Pointed from two ends | Fusiform | 2 celled actinomycetes conidia | (19–20) 19.5 | (6–7) 6.5 |
CS-FSD0149 | Pointed from one end | Fusiform | 2 celled actinomycetes conidia | (20–22) 21 | (2–6) 4 |
CS-FSD143 | Pointed from one end | Fusiform | Single-cell actinomycetes conidia | (15–18) 16.5 | (7–7) 7 |
CS-FSD146 | Pointed from one end | Hyaline | Single-cell actinomycetes conidia | (16–17) 16.5 | (5–7) 6 |
S. No. | Variety | Percent Scab Incidence | Percent Scab Index | Reaction |
---|---|---|---|---|
1 | Cardinal | 37.46 | 15.91 | Medium susceptible |
2 | Santee | 49.63 | 20.5 | Susceptible |
3 | Lady Rosetta | 17.55 | 5.90 | Less susceptible |
4 | Kuroda | 56.35 | 20.65 | Susceptible |
5 | Tourag | 46.25 | 18.77 | Medium susceptible |
6 | Asterix | 2.90 | 1.47 | Resistant |
7 | Diamant | 41.55 | 11.38 | Less susceptible |
8 | Faisalabad White | 22.53 | 1.08 | Resistant |
9 | Faisalabad red | 75.24 | 25.18 | Highly susceptible |
10 | Sadaf | 33.38 | 7.09 | Less susceptible |
11 | Control | 12.01 | 6.00 | Resistant |
Varieties | Percent Disease Incidence | Percent Scab Index | No. of Tubers |
---|---|---|---|
Kuroda | 56.48 a | 20.71 ab | 6.01 a |
Santee | 50.61 b | 21.16 a | 5.66 a |
Faisalabad red | 47.75 c | 20.64 ab | 5.01 a |
Tourag | 45.91 c | 18.56 bc | 5.66 a |
Diamant | 41.38 d | 11.13 d | 6.01 a |
Cardinal | 38.11 e | 16.51 c | 4.66 a |
Sadaf | 33.83 f | 7.09 e | 5.66 a |
Asterix | 2.94 g | 1.55 f | 5.33 a |
Faisalabad white | 2.81 g | 1.25 f | 5.66 a |
Lady Rosetta | 2.35 g | 1.05 f | 5.01 a |
Varieties | Root Weight (g) | Shoot Weight (g) | Root Length (cm) | Shoot Length (cm) |
---|---|---|---|---|
Kuroda | 3.64 bc | 9.76 bc | 20.66 ab | 30.66 c |
Santee | 3.24 bc | 9.64 bc | 19.66 ab | 39.66 ab |
Faisalabad red | 2.22 c | 6.83 d | 19.66 ab | 41.01 ab |
Tourag | 4.24 abc | 10.44 bc | 21.33 a | 41.33 ab |
Diamant | 4.81 ab | 11.54 ab | 19.01 ab | 34.01 bc |
Cardinal | 5.85 a | 14.78 a | 16.01 b | 40.33 ab |
Sadaf | 3.56 bc | 10.61 bc | 16.33 ab | 40.01 ab |
Asterix | 4.96 ab | 12.37 ab | 20.33 ab | 26.66 c |
Faisalabad white | 2.59 c | 7.36 cd | 18.33 ab | 30.66 c |
Lady Rosetta | 3.81 ab | 10.63 bc | 21.33 a | 46.01 a |
Isolates | Identified Species | Similarity% | Accession Number |
---|---|---|---|
CS-TAX01 | Streptomyces scabies | 100% | M57297.1 |
CS-TAX002 | Streptomyces scabies | 98% | AF031232.1 |
CS-TAX004 | Streptomyces scabies | 100% | AM293590.1 |
CS-TAX08 | Streptomyces scabies | 99% | HM018077.1 |
CS-TAX13 | Streptomyces griseoflavus | 96% | JX284407.1 |
CS-HAZ14 | Streptomyces europaeiscabiei | 90% | HQ441817.1 |
CS-HAZ62 | Streptomyces europaeiscabiei | 89% | AY207595.1 |
CS-SIAL060, | Streptomyces scabies | 99% | AY207602.1 |
CS-SIAL70 | Streptomyces scabies | 98% | AB301479.1 |
CS-SIAL72 | Streptomyces scabies | 99% | Y15497.1 |
S. No | Variety Name | Location/Source |
---|---|---|
1 | Cardinal | National agricultural research institute (NARC), Islamabad |
2 | Santee | National agricultural research institute (NARC), Islamabad |
3 | Lady Rosetta | National agricultural research institute (NARC), Islamabad |
4 | Kuroda | National agricultural research institute (NARC), Islamabad |
5 | Tourag | National agricultural research institute (NARC), Islamabad |
6 | Asterix | Potato Research Institute (PRI), Sahiwal |
7 | Diamant | Potato Research Institute (PRI), Sahiwal |
8 | Faisalabad white | Potato Research Institute (PRI), Sahiwal |
9 | Faisalabad red | Potato Research Institute (PRI), Sahiwal |
10 | Sadaf | Potato Research Institute (PRI), Sahiwal |
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Ismail, S.; Jiang, B.; Nasimi, Z.; Inam-ul-Haq, M.; Yamamoto, N.; Danso Ofori, A.; Khan, N.; Arshad, M.; Abbas, K.; Zheng, A. Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm. Pathogens 2020, 9, 760. https://doi.org/10.3390/pathogens9090760
Ismail S, Jiang B, Nasimi Z, Inam-ul-Haq M, Yamamoto N, Danso Ofori A, Khan N, Arshad M, Abbas K, Zheng A. Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm. Pathogens. 2020; 9(9):760. https://doi.org/10.3390/pathogens9090760
Chicago/Turabian StyleIsmail, Sohaib, Bo Jiang, Zohreh Nasimi, M. Inam-ul-Haq, Naoki Yamamoto, Andrews Danso Ofori, Nawab Khan, Muhammad Arshad, Kumail Abbas, and Aiping Zheng. 2020. "Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm" Pathogens 9, no. 9: 760. https://doi.org/10.3390/pathogens9090760