The Immune Efficacy of Inactivated Pseudorabies Vaccine Prepared from FJ-2012ΔgE/gI Strain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Suckling Pig and Sites
2.2. Experimental Vaccines and Main Reagents
2.3. Experimental Pigs Groups and Immunization Procedures
2.4. Experimental Pigs Challenged Procedures and Clinical Record
2.5. PRV gE, gB Antibody Test, and Neutralizing Antibody Detection
2.6. Necropsy, Tissue Sampling, and Histological Analysis
2.7. Establishment of PRV qPCR Method and Tissue Viral Load Analysis
2.8. Statistical Analysis
2.9. Ethical Statement
3. Results
3.1. PRV gB and gE Antibody Production in Piglets
3.2. Virus Neutralizing Antibodies of PRV Induced by Vaccines in Piglets
3.3. Protection of Vaccinated Piglets against Virulent Challenge
3.4. Necropsy and Histological Analysis
3.5. Tissue Viral Load Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Vaccine Type | Gene-Deleted Vaccines | Vaccine Strain | Phenotype | References |
---|---|---|---|---|
Inactivated | Single gene-deleted | AH02LA, HN1201 (licensed) | gE-deleted | [18,19] |
Double gene-deleted | ZJ01 | gE/gI-deleted | [20] | |
Live-attenuated | Single gene-deleted | TJ | gE-deleted | [21] |
Double gene-deleted | JS-2012 | gE/gI-deleted | [22] | |
Double gene-deleted | JS-2012 | gE/US2-deleted | [23] | |
Double gene-deleted | XJ, AH02LA | gE/TK-deleted | [24,25] | |
Triple-gene-deleted | ZJ01, MX, HN1201, HeN1, NY, and TJ | gE/gI/TK-deleted | [26,27,28,29,30,31] | |
Four-gene-deleted (natural losses) | C (licensed) | gE/gI/US9/US2-deleted | [32] |
Groups | Immunization Time | Challenge Time | |
---|---|---|---|
0 Days | 21 Days | 42 Days | |
FJ-2012-GEL* | √ | √ | √ |
FJ-2012-VG* | √ | √ | √ |
vPRV* | √ | √ | √ |
K61 | √ | × | √ |
Unimmunized challenged | √ | √ | √ |
Control | × | × | × |
Groups | Lung | Kidney | Tonsil | Brain | Spleen |
---|---|---|---|---|---|
FJ-2012-GEL* | 0 a/5 b | 0/5 | 0/5 | 0/5 | 0/5 |
FJ-2012-VG* | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
vPRV* | 2/5 | 0/5 | 0/5 | 0/5 | 0/5 |
K61 | 3/5 | 0/5 | 2/5 | 1/5 | 0/5 |
Unimmunized challenged | 5/5 | 3/5 | 3/5 | 5/5 | 2/5 |
Control | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Groups | MLS ± SD § | ||||
---|---|---|---|---|---|
Lung † | Kidney ‡ | Tonsil † | Brain † | Spleen † | |
FJ-2012-GEL* | 0.00 ± 0.00 Bc‖ | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Cc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc |
FJ-2012-VG* | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Cc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc |
vPRV* | 2.00 ± 1.00 BCb | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Cc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc |
K61 | 3.67 ± 1.15 ACb | 1.33 ± 0.58 Ab | 2.33 ± 0.58 Bb | 1.67 ± 1.16 Bb | 2.33 ± 1.53 ABb |
Unimmunized challenged | 5.67 ± 0.58 Aa | 2.33 ± 0.58 Aa | 5.00 ± 1.00 Aa | 4.67 ± 1.16 Aa | 4.33 ± 0.58 Aa |
Control | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Cc | 0.00 ± 0.00 Bc | 0.00 ± 0.00 Bc |
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Chen, Q.-Y.; Wu, X.-M.; Che, Y.-L.; Chen, R.-J.; Hou, B.; Wang, C.-Y.; Wang, L.-B.; Zhou, L.-J. The Immune Efficacy of Inactivated Pseudorabies Vaccine Prepared from FJ-2012ΔgE/gI Strain. Microorganisms 2022, 10, 1880. https://doi.org/10.3390/microorganisms10101880
Chen Q-Y, Wu X-M, Che Y-L, Chen R-J, Hou B, Wang C-Y, Wang L-B, Zhou L-J. The Immune Efficacy of Inactivated Pseudorabies Vaccine Prepared from FJ-2012ΔgE/gI Strain. Microorganisms. 2022; 10(10):1880. https://doi.org/10.3390/microorganisms10101880
Chicago/Turabian StyleChen, Qiu-Yong, Xue-Min Wu, Yong-Liang Che, Ru-Jing Chen, Bo Hou, Chen-Yan Wang, Long-Bai Wang, and Lun-Jiang Zhou. 2022. "The Immune Efficacy of Inactivated Pseudorabies Vaccine Prepared from FJ-2012ΔgE/gI Strain" Microorganisms 10, no. 10: 1880. https://doi.org/10.3390/microorganisms10101880