Genetic Analysis of a Large-Scale Phaeocystis globosa Bloom Offshore Qingdao, China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Field Sampling
2.2. Morphological Observation
2.3. DNA Preparation
2.4. PCR Amplification, Cloned and Sanger Sequencing
2.5. Phylogenetic Analyses
2.6. Network Construction Using TCS
2.7. Correlation Analysis between Genotypes and Environmental Factors
3. Results
3.1. Description of the First Large-Scale P. globosa Bloom Recorded Offshore Qingdao
3.2. Genetic Dissection of P. globosa Bloom Development Using pgcp1
3.3. Phaeocystis globosa Genotype Composition during Bloom Development Revealed Using cox1
3.4. Environmental Factors and Their Influence on P. globosa Genotype Composition
4. Discussion
4.1. An Unusual P. globosa Bloom Event Observed Offshore Qingdao, China
4.2. Genetic Uniqueness of P. globosa in Bloom Development
4.3. Source of P. globosa Strains Driving the Bloom Offshore Qingdao, China
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Genotype | Geographic Origin of Water Samples | Geographic Origin of Strains | GenBank Accession or SRA Read Numbers |
---|---|---|---|
pgeno1 | Qingdao, China | Beibu Gulf, China; Fujian, China | ON080925 |
pgeno2 | Qingdao, China | Beibu Gulf, China; Lianyungang, China; Daya Bay, China; Fujian, China | ON080928 |
pgeno3 | Qingdao, China | Beibu Gulf, China | ON080929 |
pgeno4 | Qingdao, China | ON080930 | |
pgeno5 | Qingdao, China | ON080931 | |
pgeno6 | Qingdao, China | ON080932 | |
pgeno7 | Qingdao, China | ON080933 | |
pgeno8 | Qingdao, China | ON080934 | |
pgeno9 | Qingdao, China | Thailand; the South China Sea; South Pacific | MT448570 |
pgeno10 | Beibu Gulf, China; the South China Sea | MT591390 | |
pgeno11 | Gulf of Mexico; Ocean North Atlantic; NA | ON080938 | |
pgeno12 | Beibu Gulf, China | MT591360 | |
pgeno13 | Ocean North Atlantic | ON080939 | |
pgeno14 | Viet Nam | MT448598 | |
pgeno15 | the South China Sea | ON080926 | |
pgeno16 | the South China Sea | ON080927 | |
pgeno17 | Ocean North Atlantic; Europe’s North Sea | NC_021637 | |
pgeno18 | NA | NA | SRR192667.99032.2 |
pgeno19 | NA | NA | SRR192554.305016.2 |
Genotype | Geographic Origin of Water Samples | Geographic Origin of Strains | GenBank Accession |
---|---|---|---|
cgeno1 | Qingdao, China; Beibu Gulf, China | Beibu Gulf, China | ON080911 |
cgeno2 | Qingdao, China; Beibu Gulf, China | Beibu Gulf, China; Lianyungang, China; Daya Bay, China; Fujian, China | ON080912 |
cgeno3 | Qingdao, China | Beibu Gulf, China | ON080913 |
cgeno4 | Qingdao, China | ON080914 | |
cgeno5 | Qingdao, China | ON080915 | |
cgeno6 | Qingdao, China | ON080916 | |
cgeno7 | Beibu Gulf, China | Beibu Gulf, China; Thailand; Viet Nam; the South China Sea; North Pacific; South Pacific | ON080935 |
cgeno8 | Ocean North Atlantic; Europe’s North Sea | KC967226 | |
cgeno9 | Ocean North Atlantic | ON080936 | |
cgeno10 | Gulf of Mexico; Ocean North Atlantic; NA | ON080937 | |
cgeno11 | Viet Nam | ON080917 | |
cgeno12 | the South China Sea | ON080918 | |
cgeno13 | Beibu Gulf, China | ON080919 | |
cgeno14 | Beibu Gulf, China | ON080920 | |
cgeno15 | Beibu Gulf, China | ON080921 | |
cgeno16 | Beibu Gulf, China | ON080922 | |
cgeno17 | Beibu Gulf, China | ON080923 | |
cgeno18 | Beibu Gulf, China | ON080924 |
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Song, H.; Wang, Y.; Ding, X.; Chen, N. Genetic Analysis of a Large-Scale Phaeocystis globosa Bloom Offshore Qingdao, China. Microorganisms 2022, 10, 1723. https://doi.org/10.3390/microorganisms10091723
Song H, Wang Y, Ding X, Chen N. Genetic Analysis of a Large-Scale Phaeocystis globosa Bloom Offshore Qingdao, China. Microorganisms. 2022; 10(9):1723. https://doi.org/10.3390/microorganisms10091723
Chicago/Turabian StyleSong, Huiyin, Yiqi Wang, Xiangxiang Ding, and Nansheng Chen. 2022. "Genetic Analysis of a Large-Scale Phaeocystis globosa Bloom Offshore Qingdao, China" Microorganisms 10, no. 9: 1723. https://doi.org/10.3390/microorganisms10091723