A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Culture Conditions
2.2. Antibacterial Activity Assay
2.3. Sequential Measurement of Bacterial Growth and Bacteriocin Production
2.4. Antibacterial Spectral Assay
2.5. PCR and DNA Sequencing to Close Plasmid Gaps
2.6. Plasmid Curing
2.7. Bacteriocin Purification
2.8. N-Terminal Amino Acid Sequencing
2.9. Mass Spectrometry Analysis
3. Results
3.1. Comparison of Bacterial Growth and Bacteriocin Production
3.2. Comparison of Antibacterial Spectra
3.3. DNA Sequencing and Plasmid Mapping
3.4. Homology Analysis of Mesentericin Y105–B105-Related Genes
3.5. Plasmid Curing
3.6. Purification of Bacteriocins
3.7. Identification of Purified Bacteriocins
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tested Bacterium | Culture Condition 2 | Antibacterial Activity (AU/mL) 3 | ||
---|---|---|---|---|
Species | Strain 1 | Strain 406 | Strain 213M0 | |
Listeria monocytogenes | VTU 206 | 37 °C, TYLG | 4969 ± 99.1 | 2695 ± 63.1 |
Listeria monocytogenes | JCM 7671 | 37 °C, TYLG | 3093 ± 111.6 | 2560 ± 105.2 |
Listeria monocytogenes | JCM 7672 | 37 °C, TYLG | 3328 ± 85.9 | 3072 ± 97.1 |
Listeria monocytogenes | JCM 7673 | 37 °C, TYLG | 3584 ± 70.1 | 2944 ± 105.2 |
Listeria monocytogenes | JCM7674 | 37 °C, TYLG | 5546 ± 188.4 | 3584 ± 105.2 |
Listeria monocytogenes | JCM 7675 | 37 °C, TYLG | 2560 ± 0.0 | 2560 ± 0.0 |
Listeria monocytogenes | JCM 7679 | 37 °C, TYLG | 5120 ± 0.0 | 5120 ± 0.0 |
Listeria monocytogenes | JCM 7680 | 37 °C, TYLG | 1706 ± 37.0 | 2560 ± 0.0 |
Listeria ivanovii subsp. ivanovii | JCM 7681T | 37 °C, TYLG | 15,360 ± 280.4 | 9386 ± 104.5 |
Enterococcus faecalis | JCM 5803T | 37 °C, MRS | 724 ± 13.6 | 600 ± 6.8 |
Enterococcus faecium | JCM 5804T | 37 °C, MRS | - | - |
Lactococcus cremoris subsp. cremoris | NBRC 100676T | 30 °C, MRS | - | - |
Lactococcus lactis subsp. lactis | NBRC 100933T | 30 °C, MRS | - | - |
Lactococcus lactis subsp. lactis | IFO 12007 | 30 °C, MRS | - | - |
Lactococcus lactis subsp. lactis | NIAI 527 | 30 °C, MRS | - | - |
Lactococcus lactis subsp. lactis | NIAI N-7 | 30 °C, MRS | - | - |
Lactococcus lactis subsp. lactis | JCM 7638 | 30 °C, MRS | - | - |
Lactococcus lactis subsp. hordniae | NBRC 100931T | 30 °C, MRS | - | - |
Leuconostoc mesenteroides subsp. cremoris | NBRC 107766T | 30 °C, MRS | 72 ± 2.5 | 32 ± 1.3 |
Leuconostoc mesenteroides subsp. dextranicum | NBRC 100495T | 30 °C, MRS | 471 ± 11.2 | 235 ± 5.5 |
Leuconostoc mesenteroides subsp. mesenteroides | NBRC 100496T | 30 °C, MRS | - | - |
Leuconostoc lactis | JCM 6123T | 30 °C, MRS | 20 ± 0.0 | 40 ± 1.4 |
Pediococcus acidilactici | JCM 8797T | 30 °C, MRS | - | - |
Pediococcus parvulus | JCM 5889T | 30 °C, MRS | - | - |
Pediococcus pentosaceus | JCM 5885 | 37 °C, MRS | - | - |
Pediococcus pentosaceus | JCM 5890T | 37 °C, MRS | - | - |
Streptococcus thermophilus | JCM 17834T | 37 °C, MRS | - | - |
Streptococcus thermophilus | JCM 20026 | 37 °C, MRS | - | - |
Weissella cibaria | JCM 12495T | 30 °C, MRS | 297 ± 8.6 | 114 ± 6.1 |
Weissella confusa | JCM 1093T | 30 °C, MRS | - | - |
Weissella paramesenteroides | JCM 9890T | 30 °C, MRS | 1768 ± 30.3 | 522 ± 8.4 |
Weissella viridescens | JCM 1174T | 37 °C, MRS | - | - |
Primer Name | Sequence (5′–3′) | Target |
---|---|---|
406-33-F1 | CCCAATACACCTTTACCACCAC | Contig-33 (pLM406A) in strain 406 |
406-33-R1 | CTTGGATTGTGGGAACAAGA | |
406-40-F1 | AGAAACTGCCCGTGATGGAAAC | Contig-40 (pLM406B) in strain 406 |
406-40-R1 | GCTGGTGTTGGATTGTCTTTGCT | |
213-26-F4 | AGCGGTTGCTATAACGGCTA | Contig-26 (pLM213M0A) in strain 213M0 |
213-26-R4 | GCTTCAAATGACGACTGCAA | |
213-26-F5 | CGAGCTTTAAAGGGTGCTGAAAAAT | Contig-26 (pLM213M0A) in strain 213M0 |
213-26-R5 | CGCTACTGAATTTCTTGTCAAGGTTGT | |
213-30-F1 | TTAGTCCGTGAGCGGTTTATGAGAG | Contig-30 (pLM213M0B) in strain 213M0 |
213-30-R1 | AATCAAGAAAGGAGCTGTGATGACG | |
213-48-F2 | TTGCGCTAATCGGTCAATGG | Contig-48 (pLM213M0C) in strain 213M0 |
213-48-R2 | GTGACCGACCGTAGGGAGACTTTAT | |
mesY-F | ACCAAAATCCATTTCCACCA | Structural gene (mesY) of mesentericin Y105 |
mesY-R | TCTGTGGAAGCATATCAGCAA |
Plasmid | Length (bp) | GC (%) | ORFs | Accession No. |
---|---|---|---|---|
pLM406A | 32,775 | 34.2 | 42 | LC832860 |
pLM406B | 23,705 | 39.4 | 24 | LC832861 |
pLM213M0A | 41,975 | 33.5 | 51 | LC832857 |
pLM213M0B | 11,669 | 33.8 | 14 | LC832858 |
pLM213M0C | 6449 | 30.2 | 8 | LC832859 |
Producer | Fraction | N-Terminal Sequence | MS [M + H]+ | Identification |
---|---|---|---|---|
Leuconostoc mesenteroides 406 | 406-I | KYYGNGVH(X)TKSG- | 3869 | Mesentericin Y105 |
406-III | KGVLGWLSMASSA- | 3463 | Oxidized mesentericin B105 | |
406-IV | KGVLGWL- | 3447 | Mesentericin B105 | |
Leuconostoc mesenteroides 213M0 | 213M0-I | KYYGNGVH(X)TKSG- | 3869 | Mesentericin Y105 |
213M0-II | HWIGDVLGAIGHV- | 4564 | Mesentericin M 1 | |
213M0-III | - | 3463 | Oxidized mesentericin B105 | |
213M0-IV | KGVLGWL- | 3447 | Mesentericin B105 |
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Hasiqimuge; Hano, C.; Arakawa, K.; Yoshida, S.; Zhao, J.; Toh, H.; Morita, H.; Miyamoto, T. A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag. Microorganisms 2024, 12, 1781. https://doi.org/10.3390/microorganisms12091781
Hasiqimuge, Hano C, Arakawa K, Yoshida S, Zhao J, Toh H, Morita H, Miyamoto T. A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag. Microorganisms. 2024; 12(9):1781. https://doi.org/10.3390/microorganisms12091781
Chicago/Turabian StyleHasiqimuge, Chihiro Hano, Kensuke Arakawa, Saki Yoshida, Junliang Zhao, Hidehiro Toh, Hidetoshi Morita, and Taku Miyamoto. 2024. "A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag" Microorganisms 12, no. 9: 1781. https://doi.org/10.3390/microorganisms12091781