Comparison of the Effectiveness of Two Different Vaccination Regimes for Avian Influenza H9N2 in Broiler Chicken
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Birds and Vaccines
2.3. Experiment Design
2.3.1. Laboratory Groups
2.3.2. Field-monitored Groups
2.4. Hemagglutination Inhibition (HI) Test
2.5. Challenge Virus
2.6. qRT-PCR for Virus Shedding
2.7. Statistical Analysis
3. Results
3.1. Different AIV-H9N2 Virus Hemagglutinin Segment Amino Acid Identity Degrees
3.2. Immune Response to Other Vaccines at 28th Day of Life
3.3. Immune Response in Groups Kept under Laboratory Conditions
3.4. Protection Following Challenge with a Wild Type H9N2 at the 28th Day of Life
3.4.1. Seroconversion 7-DPC with Recent Middle Eastern H9N2
3.4.2. Virus Shedding Following Challenge with H9N2 at 28 Days of Life
3.5. Immune Response in Groups Kept under Field Condition
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group No. | Bird No. | Vaccine Regime | Challenge at 28 Day of Age | Assessment of Protection | |||
---|---|---|---|---|---|---|---|
Vaccine Type | Age/Days | Dose/mL | |||||
Experiment 1 lab experiment | G-1 | 20 | A | 1 | 0.3 | +++ | Follow up of immune response of vaccinated birds at weeks post vaccination to H9, H5, ND, IBD, and IB vaccines using HI and ELISA tests. Viral shedding detected by real-time PCR. |
G-2 | 20 | B | 1 | 0.3 | +++ | ||
G-3 | 20 | A | 7 | 0.3 | +++ | ||
G-4 | 20 | B | 7 | 0.3 | +++ | ||
G-5 | 20 | - | ------ | ---- | +++ | ||
G-6 | 20 | - | ------ | --- | ----- | ||
Experiment 2 field group | G-7 | 27K | A | 1 | 0.3 | - | Follow up of immune response of vaccinated birds on weekly basis. Measure of performance parameters and field exposure. Natural exposure monitoring by RT-PCR. |
G-8 | 27K | B | 1 | 0.3 | - | ||
G-9 | 27K | A | 6 | 0.3 | - | ||
G-10 | 27K | B | 6 | 0.3 | - |
Items | Alg. 2017 | Egypt 2018 | Iraq 2017 | KSA 2018 | Leb. 2017 | Libya 2015 | Mor. 2018 | Pak. 2018 | Tun. 2015 | UEA 2017 | Vaccine A | Vaccine B |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Alg./2017 | 94.59 | 94.78 | 96.39 | 94.59 | 96.39 | 99.28 | 91.88 | 91.76 | 98.10 | 93.68 | 92.24 | |
Egypt/2018 | 94.59 | 94.79 | 93.68 | 94.64 | 95.54 | 94.64 | 92.54 | 94.58 | 96.46 | 98.86 | 91.79 | |
Iraq/2017 | 94.78 | 94.79 | 94.34 | 93.32 | 93.93 | 93.21 | 94.46 | 93.11 | 93.04 | 94.43 | 90.71 | |
KSA/2018 | 96.39 | 93.68 | 91.34 | 94.22 | 95.49 | 96.57 | 90.43 | 92.31 | 96.39 | 94.78 | 91.88 | |
Leb./2017 | 94.59 | 94.64 | 92.32 | 94.22 | 96.79 | 94.82 | 90.89 | 93.41 | 94.64 | 94.75 | 92.86 | |
Libya/2015 | 96.39 | 95.54 | 93.93 | 95.49 | 96.79 | 96.25 | 92.86 | 95.33 | 96.07 | 94.64 | 93.93 | |
Mor./2018 | 99.28 | 94.64 | 93.21 | 96.57 | 94.82 | 96.25 | 91.96 | 92.31 | 99.82 | 94.75 | 92.14 | |
Pak./2018 | 91.88 | 90.54 | 94.46 | 90.43 | 90.89 | 92.86 | 91.96 | 89.84 | 91.79 | 94.18 | 89.11 | |
Tun./2015 | 91.76 | 92.58 | 90.11 | 92.31 | 93.41 | 95.33 | 92.31 | 89.84 | 92.31 | 95.48 | 89.56 | |
UEA/2017 | 98.10 | 94.46 | 93.04 | 96.39 | 94.64 | 96.07 | 99.82 | 91.79 | 92.31 | 96.57 | 91.96 | |
Vaccine A | 94.68 | 98.86 | 94.43 | 94.78 | 93.75 | 94.64 | 94.75 | 94.18 | 95.48 | 96.57 | 90.61 | |
Vaccine B | 92.24 | 91.79 | 90.71 | 91.88 | 92.86 | 93.93 | 92.14 | 89.11 | 89.56 | 91.96 | 91.61 |
Group No. | Bird No. | Vaccine Regime | ELISA Mean Titers | HI Titer Log2 | |||
---|---|---|---|---|---|---|---|
Vaccine Type | Age/days | Dose/mL | IBD | AI-H5 | ND | ||
28 Days | 28 Days | 28 Days | |||||
G-1 | 20 | A | 1 | 0.3 | 17,553 ± 1105 | 3.7 ± 0.51 | 4.8 ± 0.72 |
G-2 | 20 | B | 1 | 0.3 | 17,703 ± 1120 | 3.8 ± 0.61 | 4.9 ± 0.61 |
G-3 | 20 | A | 7 | 0.3 | 17,612 ± 1090 | 3.6 ± 0.42 | 4.7 ± 0.66 |
G-4 | 20 | B | 7 | 0.3 | 16,217 ± 1220 | 3.6 ± 0.52 | 4.8 ± 0.62 |
G-5 | 20 | - | - | - | 17,533 ± 1140 | 3.7 ± 0.65 | 4.9 ± 0.56 |
G-6 | 20 | - | - | - | 17,533 ± 1170 | 3.7 ± 0.72 | 4.8 ± 0.81 |
Days of Life | Antigen Type | Experimental Groups | |||||
---|---|---|---|---|---|---|---|
G-1 | G-2 | G-3 | G-4 | G-5 | G-6 | ||
D-7 | Antigen A | 3.2 ± 0.79 | 3.2 ± 0.79 | 3.2 ± 0.79 | 3.2 ± 0.79 | 3.2 ± 0.79 | 3.2 ± 0.79 |
Antigen B | 2.6 ± 0.69 | 2.6 ± 0.69 | 2.6 ± 0.69 | 2.6 ± 0.69 | 2.6 ± 0.69 | 2.6 ± 0.69 | |
D-14 | Antigen A | 2.8 ± 0.54 | 1.8 ± 0.42 | 1.8 ± 0.49 | 1.8 ± 0.56 | 1.8 ± 0.48 | 1.8 ± 0.32 |
Antigen B | 2.1 ± 0.44 | 2.2 ± 0.54 | 1.6 ± 0.74 | 1.6 ± 0.64 | 1.6 ± 0.74 | 1.6 ± 0.64 | |
D-21 | Antigen A | 2.6 ± 0.55 | 1.8 ± 0.67 | 2.4 ± 0.52 | 2.2 ± 0.42 | nd | nd |
Antigen B | 2.2 ± 0.54 | 2.3 ± 0.64 | 2.1 ± 0.54 | 2.1 ± 0.54 | nd | nd | |
D-28 | Antigen A | 4.1 ± 0.71 | 2.9 ± 0.59 | 4.4 ± 0.57 | 3.6 ± 0.46 | nd | nd |
Antigen B | 3.1 ± 0.44 | 3.1 ± 0.54 | 3.4 ± 0.64 | 3.2 ± 0.64 | nd | nd | |
D-35 | Antigen A | 5.4 ± 0.61 | 4.0 ± 0.57 | 6.4 ± 0.52 | 4.5 ± 0.67 | 8.3 ± 0.65 | nd |
Antigen B | 4.2 ± 0.54 | 4.1 ± 0.48 | 5.1 ± 0.45 | 4.9 ± 0.64 | 7.4 ± 0.82 | nd |
Group No. | Bird No. | Vaccine Regime | GMT Log2 HI Titer (n = 10) | |||
---|---|---|---|---|---|---|
Vaccine Type | Age/days | Dose/mL | 7 Days Post-Challenge | |||
Local Antigen | Imported Antigen | |||||
1 | 10 | A | 1 | 0.3 | 4.1 ± 1.2 * | 3.2 ± 1.21 * |
2 | 10 | B | 1 | 0.3 | 7.0 ± 2.57 * | 5.9 ± 2.57 * |
3 | 10 | A | 7 | 0.3 | 5.1 ± 0.92 * | 4.8 ± 0.89 * |
4 | 10 | B | 7 | 0.3 | 6.5 ± 1.67 * | 5.5 ± 1.87 * |
5 | 10 | - | - | - | 8.3 ± 0.65 * | 7.4 ± 0.82 * |
6 | 10 | - | - | - | - | - |
Group No. | Vaccinal Regime | Assessment of Protection | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Vaccine Type | Vaccine Age | 3-DPC | 7-DPC | |||||||
Tracheal Swabs | Cloacal Swabs | Tracheal Swabs | Cloacal Swabs | |||||||
No./EID50 | % | No./EID50 | % | No./EID50 | % | No./EID50 | % | |||
G-1 | A | 1 | 4/10 (2.1 ± 0.9) b | 40% | 3/10 (1.5 ± 0.3) c | 30% | 3/10 (1.8 ± 0.7) b | 30% | 3/10 (1.8 ± 0.8) b | 30% |
G-2 | B | 1 | 6/10 (2.8 ± 0.8) c | 60% | 4/10 (2.1 ± 0.4) c | 40% | 3/10 (2.3 ± 1.1) c | 30% | 4/10 (2.5 ± 0.8) c | 40% |
G-3 | A | 7 | 2/10 (1.6 ± 0.6) a | 20% | 2/10 (1.1 ± 0.3) a | 20% | 1/10 (1.3 ± 0.0) a | 10% | 2/10 (1.8 ± 0.5) a | 20% |
G-4 | B | 7 | 4/10 (2.4 ± 0.7) c | 40% | 3/10 (1.9 ± 0.3) c | 30% | 2/10 (1.9 ± 0.7) c | 20% | 3/10 (2.1 ± 0.6) c | 30% |
G-5 | - | - | 10/10 (3.1 ± 0.9) d | 100% | 6/10 (2.1 ± 0.3) c | 60% | 7/10 (2.3 ± 1.9) c | 70% | 10/10 (3.1 ± 1.8) d | 100% |
G-6 | - | - | nd | - | nd | - | nd | - | nd | - |
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Talat, S.; Abouelmaatti, R.R.; Almeer, R.; Abdel-Daim, M.M.; Elfeil, W.K. Comparison of the Effectiveness of Two Different Vaccination Regimes for Avian Influenza H9N2 in Broiler Chicken. Animals 2020, 10, 1875. https://doi.org/10.3390/ani10101875
Talat S, Abouelmaatti RR, Almeer R, Abdel-Daim MM, Elfeil WK. Comparison of the Effectiveness of Two Different Vaccination Regimes for Avian Influenza H9N2 in Broiler Chicken. Animals. 2020; 10(10):1875. https://doi.org/10.3390/ani10101875
Chicago/Turabian StyleTalat, Shaimaa, Reham R. Abouelmaatti, Rafa Almeer, Mohamed M. Abdel-Daim, and Wael K. Elfeil. 2020. "Comparison of the Effectiveness of Two Different Vaccination Regimes for Avian Influenza H9N2 in Broiler Chicken" Animals 10, no. 10: 1875. https://doi.org/10.3390/ani10101875