Animals

18 pages, 1192 KB

Open AccessArticle

Modulation of Milk Source Differences on Immunity, Nutritional Physiology and Intestinal Microbiota in Neonatal Piglets

by Junhong Liu, Miaomiao Bai, Shanshan Wang, Yihui Zhang, Changfeng Peng, Yirui Shao, Xia Xiong, Yueyao Xing and Hongnan Liu

Animals 2025, 15(21), 3104; https://doi.org/10.3390/ani15213104 (registering DOI) - 25 Oct 2025

Abstract

Milk sources directly influence digestion, absorption, and overall nutrient utilization during early infant nutrition. Goat milk features a nutritional composition and digestive properties that are more similar to human breast milk. This study aimed to investigate the effects of different milk sources on [...] Read more.

Milk sources directly influence digestion, absorption, and overall nutrient utilization during early infant nutrition. Goat milk features a nutritional composition and digestive properties that are more similar to human breast milk. This study aimed to investigate the effects of different milk sources on the immunity, amino acid and fatty acid metabolism, and intestinal microbiota in neonatal piglets. Sixteen 7-day-old suckling piglets were randomly allocated into two groups (eight replicates/group, one piglet/replicate) and fed with standard formula milk powder (CON) and goat milk formula powder (GMF). The formal experiment lasted for 14 days. Results showed that compared with the CON group, the GMF group showed a significant increase (p < 0.05) in the final weight, the serum levels of immunoglobulin A (IgA), IgG, IgM and C-reactive protein (CRP4), and intestinal trypsin content. Additionally, the GMF group had higher (p < 0.05) serum essential and non-essential amino acid and fatty acid levels, and had trends toward upregulation (0.05 < p < 0.1) in hepatic mRNA expression of spermine N1-acetyltransferase 1 (SAT1), duodenal peptide transporter 1 (PePT1), and jejunal cationic amino acid transporter 1 (CAT1). Microbiome sequencing revealed that GMF enhanced intestinal microbial richness and diversity and increased concentrations of acetic and propionic acids (p < 0.05). In conclusion, GMF suggests a potential improvement in the growth performance by enhancing immunity, amino acid and fatty acid metabolism and optimizing intestinal microbiota composition in neonatal piglets. These findings further support the favorable nutritional properties and tolerability of GMF in early-life nutrition. Full article

(This article belongs to the Special Issue Feeding Strategies to Improve the Health or Development of Piglets)

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34 pages, 5899 KB

Open AccessReview

Conservation of Native Livestock Breeds in Russia: Current State and Promising Prospects

by Natalia A. Zinovieva, Tatiana E. Deniskova, Veronika R. Kharzinova, Vugar A. Bagirov, Michael N. Romanov, Valeriya V. Volkova, Dinara S. Grishina, Alexandra S. Abdelmanova, Igor V. Gusev, Ivan M. Shchukin, Vladimir I. Trukhachev and Oksana I. Boronetskaya

Animals 2025, 15(21), 3103; https://doi.org/10.3390/ani15213103 (registering DOI) - 25 Oct 2025

Abstract

This review summarizes and analyzes the current status and trends in conservation of agricultural AnGR in Russia. The biodiversity of farm animal species in Russia is significant and is represented by 507 breeds, including 236 aboriginal ones. Based on a summary of global [...] Read more.

This review summarizes and analyzes the current status and trends in conservation of agricultural AnGR in Russia. The biodiversity of farm animal species in Russia is significant and is represented by 507 breeds, including 236 aboriginal ones. Based on a summary of global experience in maintaining genetic material of various types, we describe here strategies for preserving native breeds in Russia. Genetic monitoring using modern technologies improves the relevance of biological collections and enables the selection of the most typical and valuable representatives of AnGR for cryopreservation. The National Center for AnGR has developed a key conservation strategy based on the integrated use of genetic and assisted reproductive technologies. This strategy has been successfully implemented in a few cattle and goat breeds. In 2025, genetic monitoring of AnGR reproduced in vivo and preserved in vitro began. These studies and practical measures in Russia play an important role in preserving the genetic diversity of native AnGR in a changing climate to ensure food security for current and future generations. Thus, owing to the joint efforts of research teams and state financial support, a positive trend has emerged in cryopreservation programs and the preservation of living bioresource collections in Russia. Full article

(This article belongs to the Special Issue Conservation of Native Animal Breeds: From Genetic Resources to Good Governance)

10 pages, 1217 KB

Open AccessArticle

Functional and Compositional Changes in Ileal Microbiota in Piglets During the Nursing Period Revealed by 16s rRNA Gene and Metagenomics

by Boxuan Yang, Pengfei Shen, Zhijian Xu, Jianbo Yang, Bo Song, Hui Jiang, Jianmin Chai, Jiangchao Zhao, Feilong Deng and Ying Li

Animals 2025, 15(21), 3102; https://doi.org/10.3390/ani15213102 (registering DOI) - 25 Oct 2025

Abstract

In piglets, the gut microbiota matures in a segment-specific manner during the nursing period, while fecal-based studies provide limited functional resolution across intestinal sites. We profiled the ileum using 16s rRNA gene sequencing and assessed segmental functions by shotgun metagenomics at selected ages. [...] Read more.

In piglets, the gut microbiota matures in a segment-specific manner during the nursing period, while fecal-based studies provide limited functional resolution across intestinal sites. We profiled the ileum using 16s rRNA gene sequencing and assessed segmental functions by shotgun metagenomics at selected ages. Ileal species richness and diversity were relatively stable across days. Lactobacillus were prominent from day 7, with stage-associated taxa including Lactobacillus johnsonii, Lactobacillus delbrueckii, Ligilactobacillus salivarius, and Limosilactobacillus pontis. Through metagenomic functional analysis, at 21 days, genes were enriched in butanoate metabolism, and Limosilactobacillus pontis as a potential probiotic played an important role in it. At day 28, metagenomic analysis indicated higher relative abundance in the ileum of pathways linked to cysteine and methionine metabolism and lysine biosynthesis, largely carried by Limosilactobacillus mucosae, Limosilactobacillus oris, and Limosilactobacillus pontis. These data describe the composition and function of the ileum in the intestines of piglets and indicate a differentiation period around day 21 to day 28. Full article

(This article belongs to the Section Pigs)

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18 pages, 9008 KB

Open AccessFeature PaperArticle

Enhanced Muscle Flavor in Male Chinese Mitten Crab (Eriocheir sinensis) Driven by Feed-Induced Reconfiguration of Intestinal Volatile Compounds

by Jin Cen, Bo Liu, Qunlan Zhou, Xiaochuan Zheng, Gangchun Xu, Hongyan Tian, Linghong Miao, Huiming Ding, Yongfeng Zhao and Cunxin Sun

Animals 2025, 15(21), 3101; https://doi.org/10.3390/ani15213101 (registering DOI) - 25 Oct 2025

Abstract

The traditional use of iced trash fish (IF) in Chinese mitten crab (Eriocheir sinensis) aquaculture raises sustainability concerns, but the shift to formulated feeds (FF—a commercial compound feed specifically designed to meet nutritional requirements by blending multiple ingredients and containing a [...] Read more.

The traditional use of iced trash fish (IF) in Chinese mitten crab (Eriocheir sinensis) aquaculture raises sustainability concerns, but the shift to formulated feeds (FF—a commercial compound feed specifically designed to meet nutritional requirements by blending multiple ingredients and containing a balance of nutrients) is often hindered by fears of compromising its prized flavor. This study aimed to comprehensively evaluate whether a commercial formulated feed could effectively replace IF without diminishing flavor quality, hypothesizing that FF would alter the intestinal volatile profile, thereby influencing muscle flavor. Male crabs were fed either IF or FF for eight weeks. Muscle flavor was assessed using sensory evaluation, electronic nose (E-nose), and gas chromatography–ion mobility spectrometry (GC–IMS). Volatile compounds in intestinal chyme were also analyzed by GC–IMS to explore potential transfer mechanisms. The results indicated that crabs fed with FF showed higher sensory scores for sweetness. Additionally, the E-nose analysis revealed a clear separation trend between dietary groups and showed markedly higher sensor response values for aromatic compounds, biogenically derived compounds and Maillard reaction products, sulfur-containing organic compounds, aliphatic hydrocarbons, total volatile organic compounds, alcohols and organic solvents, and alkenes in the FF group compared to the IF group. Thirty-four volatiles were discovered in the muscle. Statistical analysis (independent samples t-test) showed that the FF group exhibited significantly elevated levels of 3-methylbutanal-M, propanal, (E)-2-pentenal, 2,3-pentanedione, and pentan-1-ol-M, whereas the IF group exhibited significantly elevated levels of 2-hexanone, dihydro-2(3H)-furanone, butyl acetate, ethyl 2-methylpropanoate, and phenol (p < 0.05). Fourty-eight volatiles were identified in the intestinal chyme. Propanal and ethyl 2-methylpropanoate were the dominant odor contributors based on correlation network analysis. Strong correlations were identified between the flavor profiles of intestinal chyme and muscle, suggesting a potential transfer or transformation of volatiles. This work provides a scientific basis for optimizing aquafeed formulations to ensure sustainable crab production without sacrificing end-flavor quality. Full article

(This article belongs to the Special Issue Advances in Aquaculture Nutrition for Sustainable Health Management)

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23 pages, 22315 KB

Open AccessArticle

Hierarchical Dual-Model Detection Framework for Spotted Seals Using Deep Learning on UAVs

by Jun Liu, Fengxiang Jin, Min Ji, Liang Qu, Juan Wang and Chen Wang

Animals 2025, 15(21), 3100; https://doi.org/10.3390/ani15213100 (registering DOI) - 25 Oct 2025

Abstract

This study introduces a hierarchical dual-model detection framework for accurately monitoring spotted seals (Phoca largha) in the Liaohe River estuary by using deep learning on Unmanned Aerial Vehicles (UAVs). To address challenges such as weak target features, background interference and limited [...] Read more.

This study introduces a hierarchical dual-model detection framework for accurately monitoring spotted seals (Phoca largha) in the Liaohe River estuary by using deep learning on Unmanned Aerial Vehicles (UAVs). To address challenges such as weak target features, background interference and limited edge computing capacity, this study deploys an optimized FF-YOLOv10 lightweight model on UAVs for rapid target localization, followed by an enhanced PP-YOLOv7 model on ground stations for precise detection. The FF-YOLOv10 model reduces computational complexity by 24.2% and increases inference speed by 33.3%, while the PP-YOLOv7 model achieves 94.2% precision with a 1.9% increase in recall rate. This framework provides an efficient and precise technical solution for the long-term ecological monitoring of marine endangered species, supporting habitat conservation policy formulation and ecosystem health assessments. Full article

(This article belongs to the Section Aquatic Animals)

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24 pages, 11929 KB

Open AccessArticle

Effects of Antimicrobial Peptides on the Growth Performance of Squabs Were Investigated Based on Microbiomics and Non-Targeted Metabolomics

by Lihuan Deng, Yingying Yao, Haiying Li, Qingqing Lu and Run Wu

Animals 2025, 15(21), 3099; https://doi.org/10.3390/ani15213099 (registering DOI) - 25 Oct 2025

Abstract

This study aims to investigate the effects of dietary supplementation with AMPs on the growth performance, antioxidant capacity, and intestinal health of squabs. Furthermore, metagenomic and metabolomic approaches were employed to identify key differential bacterial species and metabolites associated with growth performance, and [...] Read more.

This study aims to investigate the effects of dietary supplementation with AMPs on the growth performance, antioxidant capacity, and intestinal health of squabs. Furthermore, metagenomic and metabolomic approaches were employed to identify key differential bacterial species and metabolites associated with growth performance, and thereby the potential mechanisms underlying the enhancement of squab growth and development by AMPs being elucidated. One hundred and twenty pairs of healthy adult White Carneau pigeons (2 years old) were randomly divided into two groups, the control group (CK, fed with basal diet) and antimicrobial peptide group (AP, fed with basal diet +200 mg/kg antimicrobial peptide), with 10 replicates per group and 6 pairs of breeding pigeons per replicate. The experiment lasted for 53 days, including 7 days of prefeeding, 18 days of incubation and 28 days of feeding. In this study, squabs were weighed at 0 and 28 days of age to evaluate growth performance. At 28 days of age, duodenal contents were collected to assess digestive enzyme activities, while jejunal and liver tissues were harvested to determine antioxidant capacity. Intestinal morphology was examined using tissue samples from the duodenum, jejunum, and ileum. Finally, ileal contents were collected for a comprehensive analysis of microbial composition and metabolite profiles in the two experimental groups, employing high-throughput sequencing and LC-MS/MS techniques. The results showed that body weight, liver total antioxidant capacity (T-AOC), jejunal malondialdehyde (MDA) content, jejunum and ileum villus height-to-crypt depth ratio (VH/CD) were significantly increased, and jejunal crypt depth (CD) was significantly decreased in the AP group at 28 days of age (p < 0.05). In addition, the microbiome data showed that Lactobacillus in the AP group was a biomarker with significant differences (p < 0.05). Metabolomics analysis showed that the steroid hormone biosynthesis pathway was significantly different between the two groups (p < 0.01). In addition, the content of potentially beneficial metabolites (Biotin, beta-Tocotrienol, 7-Chloro-L-tryptophan and Dihydrozeatin) was significantly increased in the AP group (p < 0.05). These results indicate that dietary AMPs can significantly improve the body weights, liver antioxidant capacity and jejunum and ileum VH/CD of squabs. Full article

(This article belongs to the Section Animal Physiology)

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26 pages, 5152 KB

Open AccessArticle

Evaluating the Adjuvant Therapeutic Effects of Probiotic Strains Lactococcus cremoris and Lacticaseibacillus paracasei on Canine Atopic Dermatitis and Their Impact on the Gut and Skin Microbiome

by Hsiao-Wen Huang, Ting-Chen Yeh, Jui-Chun Hsieh, Ching-Wen Tsai, Ya-Jane Lee and Ming-Ju Chen

Animals 2025, 15(21), 3098; https://doi.org/10.3390/ani15213098 (registering DOI) - 24 Oct 2025

Abstract

This study investigated the therapeutic potential of a novel probiotic combination consisting of Lactococcus cremoris subsp. cremoris MP01 and Lacticaseibacillus paracasei subsp. paracasei MP02, collectively referred to as LCP, in the treatment of canine atopic dermatitis (CAD). In a 60-day open-label, single-arm trial [...] Read more.

This study investigated the therapeutic potential of a novel probiotic combination consisting of Lactococcus cremoris subsp. cremoris MP01 and Lacticaseibacillus paracasei subsp. paracasei MP02, collectively referred to as LCP, in the treatment of canine atopic dermatitis (CAD). In a 60-day open-label, single-arm trial involving eight dogs, notable clinical improvements were observed following daily LCP treatment, as evidenced by decreasing trends in Canine Atopic Dermatitis Extent and Severity Index and Pruritus Visual Analogue Scale scores, as well as a significant reduction in serum immunoglobulin E levels (p < 0.05). Microbiome and short-chain fatty acid (SCFA) analyses were subsequently conducted in a representative subset of six dogs to explore the effects of LCP on the fecal and skin microbial ecosystems. Concomitant alterations in gut and skin microbiome were observed, including a significant reduction in abundance of Erysipelotrichaceae (p < 0.05) and non-significant decreasing trends in Romboutsia, Escherichia/Shigella spp., and Shigella flexneri, along with a trend toward increased SCFA production. Functional prediction using PICRUSt suggested potential involvement of immune- and infection-related signaling pathways, including those associated with nucleotide-binding oligomerization domain-like receptors, retinoic acid-inducible gene I-like receptors and Shigellosis, supporting the hypothesis that LCP may exert its effects through modulation of the gut–skin axis. These findings support LCP as a safe and promising adjunct therapy for CAD, offering a novel microbiome-targeted approach targeting both clinical symptoms and underlying dysbiosis. Further investigation is warranted to optimize probiotic formulations and better understand the mechanisms underlying microbiome-mediated immune modulation in canine allergy. Full article

(This article belongs to the Special Issue Nutrition, Physiology and Metabolism of Companion Animals)

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18 pages, 5581 KB

Open AccessArticle

A New Strategy to Identify Naturally Presenting SLA-I Bound Peptides Derived from the O Serotype of Foot-and-Mouth Disease Virus, by Mild Acid Elution in a VP1 Stably Expressed PK15 Cell Line

by Yong-Yu Gao, Zong-Hui Zhang, Chen-Jun Sang, Yong Han, Yu-Die Cao, Yue Tang, Gui-Xue Hu, Zi-Bin Li and Feng-Shan Gao

Animals 2025, 15(21), 3097; https://doi.org/10.3390/ani15213097 (registering DOI) - 24 Oct 2025

Abstract

Multi-epitopes of FMDV can be used to develop a novel vaccine. Determining how to screen naturally presenting epitope peptides derived from FMDV is crucial for advancing progress in this area. In this study, a transient expression plasmid named pEGFP-N1-VP1 was transfected into Porcine [...] Read more.

Multi-epitopes of FMDV can be used to develop a novel vaccine. Determining how to screen naturally presenting epitope peptides derived from FMDV is crucial for advancing progress in this area. In this study, a transient expression plasmid named pEGFP-N1-VP1 was transfected into Porcine Kidney Epithelial cells 15 (PK15). The positive cells that stably expressed the O-VP1 gene of FMDV were screened with gradient concentrations of G418 (Geneticin). The constructed pEGFP-N1-VP1/PK15 cell line was eluted by pH 3.3 phosphate buffer to isolate the eluted peptides, followed by desalting, liquid chromatography–tandem mass spectrometry (LC–MS/MS), a flow cytometric analysis of SLA-I expression, and an ELISA detection of SLA-I bound peptides. It was demonstrated that a PK15 cell line stably expressing the VP1 gene was initially screened out at 500 μg/mL of G418, followed by culturing at 300 μg/mL. The O-VP1 expression was identified using an image analysis system, RT-PCR, and Western blot analysis. Thirty-seven peptides derived from O-VP1 were eluted from the constructed cell line. The flow cytometric analysis and ELISA detection results showed that the eluted peptides were associated with SLA-I and bound. This is the first known study to construct a cell line for screening naturally presenting antigenic peptides derived from the O serotype of FMDV. Full article

(This article belongs to the Section Pigs)

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32 pages, 14182 KB

Open AccessArticle

Effects of Soybean Meal Replacement on Growth Performance, Rumen Fermentation, Rumen Microorganisms, and Metabolites in Dumont Lambs

by Henan Lu, Hairong Wang, Boyang Li, Zenghao Lv, Shufang Li, Yuhao Xia and Lina Wang

Animals 2025, 15(21), 3096; https://doi.org/10.3390/ani15213096 (registering DOI) - 24 Oct 2025

Abstract

This study investigated the effects of replacing part of the soybean meal in the diet of Dumont lambs with urea, rapeseed meal, and cottonseed meal on their growth performance and rumen fermentation and combined rumen microbial metagenomics and metabolomics to explain the reasons [...] Read more.

This study investigated the effects of replacing part of the soybean meal in the diet of Dumont lambs with urea, rapeseed meal, and cottonseed meal on their growth performance and rumen fermentation and combined rumen microbial metagenomics and metabolomics to explain the reasons for the changes in phenotypic data. Twenty-four healthy male Dumont lambs were divided into four groups: soybean meal group (T1, control group), group with 1.5% urea replacing 6.4% soybean meal (T2), group with 1% urea replacing 4.3% soybean meal (T3), and group with 1% urea + 6.6% cottonseed meal +5% rapeseed meal replacing all soybean meal (19%) (T4), following the principle of equal energy and nitrogen. Urea, rapeseed meal, and cottonseed meal have different degradation rates in the rumen, primarily stimulating arginine biosynthesis, sulphur metabolism, and carbon fixation in photosynthetic organisms through Prevotella genus mediation, thereby influencing the accumulation of metabolites such as 9,10-DiHOME, DG (PGJ2/a-15:0/0:0), isonicotinate and taxifolin, affecting rumen fermentation. Compared with the T1 group, the T2 group showed significantly increased ammonia nitrogen (NH₃-N) and microbial protein (MCP) content (p < 0.01) and improved fructose and mannose metabolic capacity (p < 0.05). The T3 group showed a significant increase in total volatile fatty acids (TVFA) and MCP content (p < 0.01), which facilitated the absorption of subsequent nutrients. In the T4 group, different degradation rates of nitrogen resources and rapeseed meal + cottonseed meal contained abundant and complementary amino acids, which improved rumen fermentation, enhanced rumen microbial and metabolite diversity, and optimized the synergistic metabolic efficiency of carbon, nitrogen and sulphur. However, the specific mechanisms of post-rumen metabolism and absorption require further investigation. Full article

(This article belongs to the Section Small Ruminants)

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14 pages, 4062 KB

Open AccessArticle

Mammary Epithelial Cell Spheroid: Stabilization Through Vascular-Wall Mesenchymal Stem Cells and Endothelial Cells Co-Culture

by Debora La Mantia, Roberta Salaroli, Biljana Petrovic, Domenico Ventrella, Augusta Zannoni, Monica Forni and Chiara Bernardini

Animals 2025, 15(21), 3095; https://doi.org/10.3390/ani15213095 (registering DOI) - 24 Oct 2025

Abstract

Over the past decade, the interest in using 3D cell culture models for studying the mammary gland in biomedical and veterinary fields has increased, but a fully functional in vitro model for domestic species is still lacking. Multiple cellular components, including epithelial cells, [...] Read more.

Over the past decade, the interest in using 3D cell culture models for studying the mammary gland in biomedical and veterinary fields has increased, but a fully functional in vitro model for domestic species is still lacking. Multiple cellular components, including epithelial cells, vascular endothelial cells, and stromal/stem cells, sustain the secretory mammary gland tissue in a well-organized 3D architecture. Considering the Göttingen Minipigs widely used for translational lactation studies, this work aimed to establish a 3D culture protocol to generate mammary heterogeneous multicellular spheroids composed of three different Göttingen Minipigs primary cells: mammary epithelial cells (mpMECs), aortic endothelial cells (mpAECs), and vascular-wall mesenchymal stem cells (mpVW-MSCs). Cells were cultured with hanging-drop (HD) and ultra-low-adherence plate (ULA) methods, evaluating aggregate formation in both monocultures and co/triple co-cultures. Brightfield area, eccentricity, viability, and cell distribution were analyzed. Results showed mpMECs formed irregular aggregates in both HD and ULA, while more compact and viable spheroids were formed when co-cultured with mpVW-MSCs and mpAECs by ULA. A well-organized cellular distribution was demonstrated by cytokeratin-18, vimentin, and e-NOS immunofluorescence analysis. In conclusion, this study established a stable 3D mammary multicellular spheroid model, representing a promising tool for future studies on hormonal modulation and mammary gland physiology. Full article

(This article belongs to the Collection Stem Cells in Domestic Animals: Applications in Health and Production)

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18 pages, 1090 KB

Open AccessArticle

Dynamic Changes in Amino Acid Release Patterns of Different Plant Protein Sources During In Vitro Digestion and Their Nutritional Value Assessment

by Yueli Fan, Zehua Kou, Jiahua Cao, Zhongshen Wang, Tianrui Zhang, Rui Han and Dongsheng Che

Animals 2025, 15(21), 3094; https://doi.org/10.3390/ani15213094 (registering DOI) - 24 Oct 2025

Abstract

A gastric–intestinal two-step enzymatic hydrolysis in vitro digestion simulation system was used to systematically investigate the digestion kinetics and amino acid release characteristics of five plant protein sources: soybean meal, rapeseed meal, corn DDGS, corn gluten meal, and corn germ meal. The results [...] Read more.

A gastric–intestinal two-step enzymatic hydrolysis in vitro digestion simulation system was used to systematically investigate the digestion kinetics and amino acid release characteristics of five plant protein sources: soybean meal, rapeseed meal, corn DDGS, corn gluten meal, and corn germ meal. The results showed that in the gastric digestion phase (120 min), the protein hydrolysis degree of soybean meal was the highest (61.8%, p < 0.001), which was 4.4 times that of corn gluten meal (14.0%). In the intestinal digestion phase (240 min), the low-molecular-weight peptide release of corn gluten meal (31.2 mg/g) was significantly higher than that of corn DDGS (17.4 mg/g), showing a “weak in the stomach but strong in the intestine” characteristic. The “nutritional value equivalence” model constructed with soybean meal as the reference showed that the gastric digestion phase equivalence of rapeseed meal was only 32.2% (significantly lower than other materials), and the intestinal digestion phase equivalence of corn gluten meal was 62.9%. This study clarified the differences in digestion characteristics and key related indicators of different plant protein sources, providing quantitative references and scientific support for the food and feed industries to precisely select protein sources according to digestion phases and optimize the formula design. Full article

(This article belongs to the Special Issue Alternative Protein Sources for Animal Feeds)

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17 pages, 695 KB

Open AccessReview

Passive Immunity Establishment Through Colostral IgG Absorption in Neonatal Ruminants: Foundation for Efficient Ruminant Production

by Chao Yang, Mei Du, Anum Ali Ahmad, Yan Cheng and Kefyalew Gebeyew

Animals 2025, 15(21), 3093; https://doi.org/10.3390/ani15213093 (registering DOI) - 24 Oct 2025

Abstract

Passive immunity, the acquisition of specific immune protection through external antibodies or immune components, is critically important for neonatal survival. In ruminants, however, neonatal hypogammaglobulinemia, a consequence of their epitheliochorial placental structure preventing prenatal antibody transfer, often leads to high morbidity and mortality. [...] Read more.

Passive immunity, the acquisition of specific immune protection through external antibodies or immune components, is critically important for neonatal survival. In ruminants, however, neonatal hypogammaglobulinemia, a consequence of their epitheliochorial placental structure preventing prenatal antibody transfer, often leads to high morbidity and mortality. Consequently, neonatal ruminants are entirely dependent on the timely consumption of colostrum to acquire sufficient immunoglobulin G (IgG) for protection. Establishing robust passive immunity is therefore a cornerstone for their survival, healthy development, and future production efficiency. This review synthesizes current knowledge on the establishment of passive immunity in neonatal ruminants. We first outline the fundamental principles of passive immunity transfer, then delve into the specific pathways and molecular mechanisms in ruminants. Key factors influencing this process are subsequently discussed. Furthermore, we highlight the long-term impact of passive immunity on adult production performance. This review aims to provide a scientific foundation for optimizing colostrum management strategies and to stimulate future research into the intricate mechanisms of IgG absorption. Full article

(This article belongs to the Special Issue Advanced Welfare-Oriented, Efficient, and High-Quality Ruminant Production: Mechanisms and Practices)

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32 pages, 758 KB

Open AccessFeature PaperReview

Alpaca (Vicugna pacos) Husbandry and Their Welfare

by Renata Pilarczyk, Małgorzata Bąkowska, Bogumiła Pilarczyk, Agnieszka Tomza-Marciniak, Beata Seremak, Jan Udała, Paulius Matusevičius and Ramutė Mišeikienė

Animals 2025, 15(21), 3092; https://doi.org/10.3390/ani15213092 (registering DOI) - 24 Oct 2025

Abstract

A key diagnostic tool in breeding practice is systematic observation: by identifying abnormalities in natural behaviour, it can highlight early signs of reduced welfare or physical and mental health issues in livestock and other animals. The aim of this paper is to review [...] Read more.

A key diagnostic tool in breeding practice is systematic observation: by identifying abnormalities in natural behaviour, it can highlight early signs of reduced welfare or physical and mental health issues in livestock and other animals. The aim of this paper is to review current literature to provide a detailed analysis of the factors affecting the physical and mental health of alpacas (Vicugna pacos) with regard to their husbandry method. It analyses various behavioural patterns such as stress reactions to strangers, isolation and grooming, as well as social interactions (hierarchy, bonding, affiliative and aggressive behaviour), reproductive activity (courtship, copulation, partner rejection), and resting and foraging rhythms. It also discusses the influence of socialisation on human–animal relationships and the role of the environment, and its enrichment, in proper behavioural functioning. It also examines the significant role played by nutrition and the environment in ensuring alpaca welfare, and how it can be maintained during grooming and training. It pays special attention to the use of behavioural tests to assess the level of trust and tameness in alpacas. Systematic behaviour observation allows a quick response to deteriorating welfare, thus facilitating more efficient herd management and reducing the risk of more serious health and behavioural problems. Full article

(This article belongs to the Section Animal Welfare)

14 pages, 3193 KB

Open AccessFeature PaperArticle

Comparative Chromosome Painting Clarifies the Intraspecific Chromosomal Variation in Two Ctenomys Species (Rodentia: Ctenomyidae)

by Thays Duarte de Oliveira, Natasha Ávila Bertocchi, Luciano Cesar Pozzobon, Ivanete de Oliveira Furo, Edivaldo Herculano Corrêa de Oliveira, Jorge C. Pereira, Malcolm A. Ferguson-Smith, Rafael Kretschmer and Thales R. O. de Freitas

Animals 2025, 15(21), 3091; https://doi.org/10.3390/ani15213091 (registering DOI) - 24 Oct 2025

Abstract

Background: Ctenomys is a subterranean rodent genus known for exhibiting the highest levels of chromosome variation, both among species (2n = 10 to 70) and within species. Ctenomys minutus is particularly notable for its extensive chromosomal diversity, comprising the greatest number of [...] Read more.

Background: Ctenomys is a subterranean rodent genus known for exhibiting the highest levels of chromosome variation, both among species (2n = 10 to 70) and within species. Ctenomys minutus is particularly notable for its extensive chromosomal diversity, comprising the greatest number of described cytotypes within this genus. In contrast, Ctenomys lami presents the highest degree of karyotypic variation within a comparatively restricted geographic range. Both species inhabit the coastal plain of southern Brazil: C. minutus occurs in dunes and sandy fields extending from Laguna (Santa Catarina State) to São José do Norte (Rio Grande do Sul State), whereas C. lami is restricted to the “Coxilha das Lombas” region, which lies parallel to the distribution of C. minutus in Rio Grande do Sul State. Despite their close evolutionary relationship and the absence of external morphological differences, the mechanism underlying their karyotypic divergence remains poorly understood. Methods: In this study, we applied whole-chromosome painting using probes from Ctenomys flamarioni to investigate chromosomal evolution in C. minutus and C. lami. Results: The resulting homology maps revealed a variety of chromosomal rearrangements that differentiate cytotypes both within and between these species. Comparative analyses demonstrated substantial karyotypic divergence from C. flamarioni, although some entire chromosomes and large chromosomal segments remained conserved between C. minutus and C. lami. Our findings underscore the critical role of chromosomal rearrangements in shaping the diversification of Ctenomys. Additionally, we identified shared chromosomal rearrangements in C. minutus and C. lami, which are likely restricted to the torquatus group. Conclusions: These rearrangements provide new insights into the processes driving chromosomal evolution in genus Ctenomys. Full article

(This article belongs to the Special Issue Vertebrate Cytogenomics: Insights into Genome and Chromosome Evolution)

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18 pages, 315 KB

Open AccessArticle

Enhanced Farrowing Efficiency and Sow Performance with Escherichia coli-Derived 6-Phytase Supplementation During Late Gestation and Lactation

by Débora Cristina Peretti, Marco Aurélio Callegari, Cleandro Pazinato Dias, Gabrieli de Souza Romano Bergamo, Bindhu Lakshmibai Vasanthakumari, Mara Cristina Ribeiro da Costa, Rafael Humberto de Carvalho and Caio Abércio da Silva

Animals 2025, 15(21), 3090; https://doi.org/10.3390/ani15213090 (registering DOI) - 24 Oct 2025

Abstract

Phytase releases phosphorus from phytate and may confer extra-phosphoric benefits in sows. We tested whether Escherichia coli-derived 6-phytase during late gestation and lactation improves sow and litter outcomes. In a randomized complete block trial, 186 TN70 sows received a phytase-free positive control [...] Read more.

Phytase releases phosphorus from phytate and may confer extra-phosphoric benefits in sows. We tested whether Escherichia coli-derived 6-phytase during late gestation and lactation improves sow and litter outcomes. In a randomized complete block trial, 186 TN70 sows received a phytase-free positive control (adequate Ca and available P) or Ca- and P-reduced diets with 500, 1500, or 2500 FTU/kg. Outcomes included sow body condition, lactation feed intake and feed conversion ratio (FCR), farrowing duration and blood glucose, piglet weaning performance and diarrhea scores, maternal serum Ca and P (farrowing, weaning), and piglet glutathione peroxidase (GPx) and superoxide dismutase (SOD; day 14). Phytase increased lactation intake by 4.4–5.6%; farrowing duration was shorter at all doses (−24.2, −23.8, and −14.8 min; up to −8.1%). Litter weaning weight rose by 6.1–8.2%, and piglet average daily gain increased by 9.1% at 2500 FTU/kg. Maternal Ca and P increased dose-responsively, especially at weaning (Ca +73% at 500–1500 FTU/kg; +140% at 2500; P +55%, +59%, +118%). Diarrhea counts declined at selected doses (e.g., scores 1–2: −17% at 500 FTU/kg), and piglet SOD decreased with dose (−8.6% to −39.3%); GPx showed modest modulation. Sow body weight, backfat, and the weaning-to-estrus interval were unchanged. In Ca- and P-reduced diets, conventional and super-dosed phytase enhanced mineral bioavailability and peripartum efficiency, supporting heavier litters without compromising sow condition. Full article

(This article belongs to the Section Animal Nutrition)

11 pages, 2347 KB

Open AccessCase Report

Use of Contrast-Enhanced Ultrasound in Suspected Traumatic or Spontaneous Renal Injury in Cats: A Case Series

by Simone Perfetti, Carolina Gai, Nikolina Linta, Giacomo Tamburini, Erika Monari, Elena Ciuffoli and Alessia Diana

Animals 2025, 15(21), 3089; https://doi.org/10.3390/ani15213089 (registering DOI) - 24 Oct 2025

Abstract

Contrast-enhanced ultrasound (CEUS) is increasingly applied in veterinary medicine as a safe, rapid, and non-invasive imaging technique for assessing renal disorders. Despite its expanding use, the literature on its application in feline renal trauma remains scarce. This retrospective study aimed to describe CEUS [...] Read more.

Contrast-enhanced ultrasound (CEUS) is increasingly applied in veterinary medicine as a safe, rapid, and non-invasive imaging technique for assessing renal disorders. Despite its expanding use, the literature on its application in feline renal trauma remains scarce. This retrospective study aimed to describe CEUS findings in cats with suspected traumatic renal injuries. Medical records were reviewed for cats that underwent both B-mode ultrasonography and CEUS, with findings confirmed by follow-up, surgery, or cytology. Three cats met the inclusion criteria. Two presented focal or multifocal renal lesions ranging from 10 to 20 mm in diameter, with heterogeneous echotexture, distortion of renal contours, and non-enhancing areas on CEUS consistent with hematomas or lacerations. The third cat showed a circumferential subcapsular halo (approximately 3–5 mm thick) with evidence of contrast leakage, compatible with limited active hemorrhage. CEUS appeared effective in identifying and characterizing renal injuries, offering valuable information to support clinical decision-making and guide both conservative and surgical management. Nevertheless, due to the limited sample size and the absence of quantitative data, these results should be considered preliminary. Further prospective studies are warranted to confirm the diagnostic performance and clinical utility of CEUS in feline renal trauma. Full article

(This article belongs to the Special Issue Advances in Canine and Feline Nephrology and Urology)

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10 pages, 1857 KB

Open AccessCommunication

The First Record of Taiga Shrew in Lithuania

by Linas Balčiauskas

Animals 2025, 15(21), 3088; https://doi.org/10.3390/ani15213088 (registering DOI) - 24 Oct 2025

Abstract

The taiga shrew (Sorex isodon), a boreal forest species widely distributed across northern Eurasia, had not previously been recorded in the Baltic states. We report the first occurrence of S. isodon in Lithuania, which extends the species range westward into the [...] Read more.

The taiga shrew (Sorex isodon), a boreal forest species widely distributed across northern Eurasia, had not previously been recorded in the Baltic states. We report the first occurrence of S. isodon in Lithuania, which extends the species range westward into the Baltic region. The specimen, which was initially misidentified as Sorex araneus, was collected in 2023 near Vilnius during non-systematic snap-trapping. A detailed examination of tail, cranial, and dental characteristics confirmed the identification as S. isodon. The individual was a non-adult male, weighing 5.8 g, and was trapped in a wet mixed forest influenced by beaver activity. This suggests that the microhabitat conditions are similar to those reported in Belarus and Ukraine. This finding extends the western boundary of S. isodon distribution by approximately 200–630 km. This study underscores the potential underreporting of this species in the Baltic region and highlights the importance of revisiting existing skull collections, conducting targeted trapping, and performing genetic analyses. Verifying additional records will clarify the habitat preferences and conservation relevance of S. isodon at the western edge of its range. Full article

(This article belongs to the Section Wildlife)

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3 pages, 158 KB

Open AccessEditorial

Happiness Is in the Field

by Dominique-Marie Votion

Animals 2025, 15(21), 3087; https://doi.org/10.3390/ani15213087 (registering DOI) - 24 Oct 2025

Abstract

Common sense suggests that ‘happiness is in the field’, as grasslands are considered environments that promote the health and well-being of grazing animals [...] Full article

(This article belongs to the Special Issue Pasture-Associated Poisoning in Grazing Animals)

17 pages, 5369 KB

Open AccessArticle

Improved Eggshell Quality in Aged Hens Through Circadian Gut Microbiota and Metabolite Changes Induced by a 28-h Ahemeral Light Cycle

by Junjie Xu, Xinxin Li, Xuelu Liu, Xinling Wu, Yihao Fan, Yichun Yao, Rongcai Zhang, Dehe Wang, Yifan Chen, Erying Hao, Yanyan Sun, Jilan Chen, Hui Chen and Lei Shi

Animals 2025, 15(21), 3086; https://doi.org/10.3390/ani15213086 (registering DOI) - 24 Oct 2025

Abstract

The decline in eggshell quality of aged laying hens represents a major economic challenge in poultry production. While a 28 h ahemeral light cycle has been shown to improve eggshell quality, its underlying mechanism remains unclear. This study randomly assigned 260 74-week-old Hy-Line [...] Read more.

The decline in eggshell quality of aged laying hens represents a major economic challenge in poultry production. While a 28 h ahemeral light cycle has been shown to improve eggshell quality, its underlying mechanism remains unclear. This study randomly assigned 260 74-week-old Hy-Line Brown laying hens to two light cycle groups, a normal 24 h cycle group (16L:8D) and a 28 h ahemeral cycle group (16L:12D). Each treatment comprised 130 hens divided into two replicate groups. The trial lasted 16 weeks. We systematically analyzed circadian rhythms of gut microbiota and serum metabolites using 16S rRNA sequencing and untargeted metabolomics. Compared with the 24 h cycle, the 28 h cycle significantly enhanced eggshell thickness by 0.04 mm and 0.02 mm, and eggshell strength by 4.19 N and 4.76 N at 79 and 84 wk, respectively. Mechanistically, the 28 h light cycle remodeled the circadian rhythms of gut microbiota, increasing their richness and diversity, and altered the rhythmic patterns of serum metabolites. We identified nine microbial genera and three hundred seventy metabolites that exhibited opposite rhythmic patterns under the two light cycles. These changes were primarily enriched in pathways related to amino acid, carbohydrate, lipid, and energy metabolism. Correlation analysis further revealed strong associations between key microbes and functional metabolites. Weissella promotes calcium deposition in eggshells through synergistic interactions with calcium chelators such as gluconic acid and threonine acid. Meanwhile, YRC22 and Paludibacter synergistically support membrane formation substances, thereby promoting the proliferation of uterine epithelial cells and eggshell formation. Our findings indicate that the 28 h ahemeral light cycle improved eggshell quality in aged hens by remodeling the circadian rhythms of gut microbiota and metabolites, thereby synergistically enhancing calcium ion absorption and uterine tissue health. This provides a novel theoretical basis and practical direction for improving late-phase egg quality through light management strategies. Full article

(This article belongs to the Section Poultry)

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