Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Equine Donors and Blood Collection
2.2. Peripheral Blood Mononuclear Cell (PBMC) Isolation
2.3. Treatments and LPS Stimulation
2.4. ELISA Assays
2.5. RNA Isolation and RT-PCR Analysis
2.6. Statistical Analysis
3. Results
3.1. Silibinin Effect on Cytokines Production
3.2. Silibinin Effect on mRNA Cytokines Expressions
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- Moore, J.N.; Vandenplas, M.L. Is it the systemic inflammatory response syndrome or endotoxemia in horses with colic? Vet. Clin. N. Am. Equine Pract. 2014, 30, 337–351. [Google Scholar] [CrossRef] [PubMed]
- Taylor, S. A review of equine sepsis. Equine Vet. Educ. 2015, 27, 99–109. [Google Scholar] [CrossRef] [PubMed]
- Vinther, A.M.; Skovgaard, K.; Heegaard, P.M.; Andersen, P.H. Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation. BMC Vet. Res. 2015, 11, 134. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Duz, M.; Marshall, J.F.; Parkin, T.D. Proportion of nonsteroidal anti-inflammatory drug prescription in equine practice. Equine Vet. J. 2019, 51, 147–153. [Google Scholar] [CrossRef]
- Lees, P.; Higgins, A.J. Clinical pharmacology and therapeutic uses of non-steroidal anti-inflammatory drugs in the horse. Equine Vet. J. 1985, 17, 83–96. [Google Scholar] [CrossRef]
- Ziegler, A.; Fogle, C.; Blikslager, A. Update on the use of cyclooxygenase-2-selective nonsteroidal anti-inflammatory drugs in horses. J. Am. Vet. Med. Assoc. 2017, 250, 1271–1274. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Danner, R.L.; Joiner, K.A.; Rubin, M.; Patterson, W.H.; Johnson, N.; Ayers, K.M.; Parrillo, J.E. Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide. Antimicrob. Agents Chemother 1989, 33, 1428–1434. [Google Scholar] [CrossRef] [Green Version]
- Kim, N.C.; Graf, T.N.; Sparacino, C.M.; Wani, M.C.; Wall, M.E. Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum). Org. Biomol. Chem. 2003, 1, 1684–1689. [Google Scholar] [CrossRef]
- Amato, A.; Terzo, S.; Mule, F. Natural Compounds as Beneficial Antioxidant Agents in Neurodegenerative Disorders: A Focus on Alzheimer’s Disease. Antioxidants 2019, 8, 608. [Google Scholar] [CrossRef] [Green Version]
- Esmaeil, N.; Anaraki, S.B.; Gharagozloo, M.; Moayedi, B. Silymarin impacts on immune system as an immunomodulator: One key for many locks. Int. Immunopharmacol. 2017, 50, 194–201. [Google Scholar] [CrossRef]
- Zheng, W.; Feng, Z.; Lou, Y.; Chen, C.; Zhang, C.; Tao, Z.; Li, H.; Cheng, L.; Ying, X. Silibinin protects against osteoarthritis through inhibiting the inflammatory response and cartilage matrix degradation in vitro and in vivo. Oncotarget 2017, 8, 99649–99665. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Federico, A.; Dallio, M.; Loguercio, C. Silymarin/Silybin and Chronic Liver Disease: A Marriage of Many Years. Molecules 2017, 22, 191. [Google Scholar] [CrossRef] [Green Version]
- Raina, K.; Agarwal, C.; Agarwal, R. Effect of silibinin in human colorectal cancer cells: Targeting the activation of NF-kappaB signaling. Mol. Carcinog 2013, 52, 195–206. [Google Scholar] [CrossRef] [Green Version]
- Tian, M.Y.; Fan, J.H.; Zhuang, Z.W.; Dai, F.; Wang, C.Y.; Hou, H.T.; Ma, Y.Z. Effects of silymarin on p65 NF-kappaB, p38 MAPK and CYP450 in LPS-induced hoof dermal inflammatory cells of dairy cows. BMC Vet. Res. 2019, 15, 127. [Google Scholar] [CrossRef] [Green Version]
- Reisinger, N.; Schaumberger, S.; Nagl, V.; Hessenberger, S.; Schatzmayr, G. Milk thistle extract and silymarin inhibit lipopolysaccharide induced lamellar separation of hoof explants in vitro. Toxins 2014, 6, 2962–2974. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hackett, E.S.; Mama, K.R.; Twedt, D.C.; Gustafson, D.L. Pharmacokinetics and safety of silibinin in horses. Am. J. Vet. Res. 2013, 74, 1327–1332. [Google Scholar] [CrossRef] [PubMed]
- Rutten, S.; Schrodl, W.; Abraham, G. Modulation of TNF-alpha, IL-1Ra and IFN-gamma in equine whole blood culture by glucocorticoids. Vet. Immunol. Immunopathol 2019, 210, 1–5. [Google Scholar] [CrossRef] [PubMed]
- Squadrito, F.; Micali, A.; Rinaldi, M.; Irrera, N.; Marini, H.; Puzzolo, D.; Pisani, A.; Lorenzini, C.; Valenti, A.; Laura, R.; et al. Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury. Front. Pharmacol. 2016, 7, 537. [Google Scholar] [CrossRef] [Green Version]
- Vendrig, J.C.; Coffeng, L.E.; Fink-Gremmels, J. Equine colostral carbohydrates reduce lipopolysaccharide-induced inflammatory responses in equine peripheral blood mononuclear cells. Equine Vet. J. Suppl 2012, 68–72. [Google Scholar] [CrossRef]
- Martin, E.M.; Messenger, K.M.; Sheats, M.K.; Jones, S.L. Misoprostol Inhibits Lipopolysaccharide-Induced Pro-inflammatory Cytokine Production by Equine Leukocytes. Front. Vet. Sci 2017, 4, 160. [Google Scholar] [CrossRef] [Green Version]
- Pizzino, G.; Irrera, N.; Bitto, A.; Pallio, G.; Mannino, F.; Arcoraci, V.; Aliquo, F.; Minutoli, L.; De Ponte, C.; D’Andrea, P.; et al. Cadmium-Induced Oxidative Stress Impairs Glycemic Control in Adolescents. Oxid Med. Cell Longev. 2017, 2017, 6341671. [Google Scholar] [CrossRef]
- MacKay, R.J. Inflammation in horses. Vet. Clin. N. Am. Equine Pract. 2000, 16, 15–27. [Google Scholar] [CrossRef]
- de la Rebiere de Pouyade, G.; Serteyn, D. The role of activated neutrophils in the early stage of equine laminitis. Vet. J. 2011, 189, 27–33. [Google Scholar] [CrossRef]
- Kawai, T.; Akira, S. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity 2011, 34, 637–650. [Google Scholar] [CrossRef] [Green Version]
- Gugliandolo, E.; Fusco, R.; Ginestra, G.; D’Amico, R.; Bisignano, C.; Mandalari, G.; Cuzzocrea, S.; Di Paola, R. Involvement of TLR4 and PPAR-alpha Receptors in Host Response and NLRP3 Inflammasome Activation, Against Pulmonary Infection with Pseudomonas Aeruginosa. Shock 2019, 51, 221–227. [Google Scholar] [CrossRef]
- Figueiredo, M.D.; Vandenplas, M.L.; Hurley, D.J.; Moore, J.N. Differential induction of MyD88- and TRIF-dependent pathways in equine monocytes by Toll-like receptor agonists. Vet. Immunol. Immunopathol. 2009, 127, 125–134. [Google Scholar] [CrossRef]
- Cudmore, L.A.; Muurlink, T.; Whittem, T.; Bailey, S.R. Effects of oral clenbuterol on the clinical and inflammatory response to endotoxaemia in the horse. Res. Vet. Sci. 2013, 94, 682–686. [Google Scholar] [CrossRef] [PubMed]
- Werners, A.H. Treatment of endotoxaemia and septicaemia in the equine patient. J. Vet. Pharmacol. Ther. 2017, 40, 1–15. [Google Scholar] [CrossRef]
- Tomlinson, J.E.; Wagner, B.; Felippe, M.J.B.; Van de Walle, G.R. Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood. Vet. Immunol. Immunopathol. 2018, 199, 22–31. [Google Scholar] [CrossRef]
- Hue, E.S.; Richard, E.A.; Fortier, C.I.; Fortier, G.D.; Paillot, R.; Raue, R.; Pronost, S.L. Equine PBMC Cytokines Profile after In Vitro alpha- and gamma-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment. Vaccines (Basel) 2017, 5, 28. [Google Scholar] [CrossRef] [Green Version]
- Zholobenko, A.; Mouithys-Mickalad, A.; Modriansky, M.; Serteyn, D.; Franck, T. Polyphenols from Silybum marianum inhibit in vitro the oxidant response of equine neutrophils and myeloperoxidase activity. J. Vet. Pharmacol. Ther. 2016, 39, 592–601. [Google Scholar] [CrossRef]
- Giorgi, V.S.; Peracoli, M.T.; Peracoli, J.C.; Witkin, S.S.; Bannwart-Castro, C.F. Silibinin modulates the NF-kappab pathway and pro-inflammatory cytokine production by mononuclear cells from preeclamptic women. J. Reprod. Immunol. 2012, 95, 67–72. [Google Scholar] [CrossRef]
- Ngkelo, A.; Meja, K.; Yeadon, M.; Adcock, I.; Kirkham, P.A. LPS induced inflammatory responses in human peripheral blood mononuclear cells is mediated through NOX4 and Gialpha dependent PI-3kinase signalling. J. Inflamm. (Lond.) 2012, 9, 1. [Google Scholar] [CrossRef] [Green Version]
- Morris, D.D.; Crowe, N.; Moore, J.N. Correlation of clinical and laboratory data with serum tumor necrosis factor activity in horses with experimentally induced endotoxemia. Am. J. Vet. Res. 1990, 51, 1935–1940. [Google Scholar] [PubMed]
- Morris, D.D.; Moore, J.N.; Crowe, N. Serum tumor necrosis factor activity in horses with colic attributable to gastrointestinal tract disease. Am. J. Vet. Res. 1991, 52, 1565–1569. [Google Scholar]
- Tadros, E.M.; Frank, N. Effects of continuous or intermittent lipopolysaccharide administration for 48 h on the systemic inflammatory response in horses. Am. J. Vet. Res. 2012, 73, 1394–1402. [Google Scholar] [CrossRef] [PubMed]
- Pusterla, N.; Magdesian, K.G.; Mapes, S.; Leutenegger, C.M. Expression of molecular markers in blood of neonatal foals with sepsis. Am. J. Vet. Res. 2006, 67, 1045–1049. [Google Scholar] [CrossRef] [Green Version]
- Brooks, A.C.; Rickards, K.J.; Cunningham, F.M. CXCL8 attenuates chemoattractant-induced equine neutrophil migration. Vet. Immunol. Immunopathol. 2011, 139, 141–147. [Google Scholar] [CrossRef] [PubMed]
- Sheats, M.K. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils. Front. Vet. Sci. 2019, 6, 69. [Google Scholar] [CrossRef]
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Gugliandolo, E.; Crupi, R.; Biondi, V.; Licata, P.; Cuzzocrea, S.; Passantino, A. Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study. Animals 2020, 10, 2022. https://doi.org/10.3390/ani10112022
Gugliandolo E, Crupi R, Biondi V, Licata P, Cuzzocrea S, Passantino A. Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study. Animals. 2020; 10(11):2022. https://doi.org/10.3390/ani10112022
Chicago/Turabian StyleGugliandolo, Enrico, Rosalia Crupi, Vito Biondi, Patrizia Licata, Salvatore Cuzzocrea, and Annamaria Passantino. 2020. "Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study" Animals 10, no. 11: 2022. https://doi.org/10.3390/ani10112022