Effects of the Application of Lactobacillus plantarum Inoculant and Potassium Sorbate on the Fermentation Quality, In Vitro Digestibility and Aerobic Stability of Total Mixed Ration Silage Based on Alfalfa Silage
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Total Mixed Ration (TMR) Silage Preparation
2.2. Chemical and Microbiological Analyses
2.3. In Vitro Incubation and Degradability Measurement
2.4. Aerobic Stability Test
2.5. Statistical Analyses
3. Results
3.1. Fermentation Quality of TMR Silages
3.2. In Vitro Degradability of TMR Silages
3.3. Aerobic Stability of TMR Silages
4. Discussion
4.1. Effects of Additives on the Fermentation Quality of TMR Silages
4.2. Effects of Additives on the In Vitro Degradability of TMR Silages
4.3. Effects of Additives on the Aerobic Stability of TMR Silages
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Item 1 | Alfalfa Silage | Corn Cob | Corn Grain | Mixed 2 Concentrate |
---|---|---|---|---|
Dry matter (g kg−1 FM) | 446.82 | 940.04 | 887.88 | 921.03 |
Crude protein (g kg−1 DM) | 145.83 | 35.87 | 83.01 | 325.29 |
Neutral detergent fiber (g kg−1 DM) | 423.30 | 810.26 | 98.50 | 263.31 |
Acid detergent fiber (g kg−1 DM) | 296.02 | 417.88 | 38.09 | 110.84 |
Ether extract (g kg−1 DM) | 41.69 | 38.21 | 48.84 | 58.09 |
Lactic acid (g kg−1 DM) | 42.47 | - | - | - |
Acetic acid (g kg−1 DM) | 4.56 | - | - | - |
Propionic acid (g kg−1 DM) | 10.00 | - | - | - |
Butyric acid (g kg−1 DM) | ND | - | - | - |
Ammonia nitrogen (g kg−1 TN) | 31.06 | - | - | - |
Item 1 | TMR |
---|---|
Ingredient composition (g kg−1 DM) | |
Alfalfa silage | 600 |
Corn cob | 60 |
Corn grain | 240 |
Mixed concentrate 2 | 100 |
Total | 1000 |
Chemical composition (g kg−1 FM) | |
Dry matter | 487.51 |
Crude protein | 146.18 |
Water soluble carbohydrate | 15.33 |
Neutral detergent fiber | 349.61 |
Acid detergent fiber | 231.16 |
Item 1 | Treatment 2 | Days of Ensiling | SEM | p-Value 3 | |||||
---|---|---|---|---|---|---|---|---|---|
0 | 7 | 14 | 30 | D | T | D × T | |||
Fermentative Characteristics | |||||||||
pH | Control | 4.65 A | 4.32 bB | 4.3b B | 4.26 bB | 0.01 | <0.001 | <0.001 | <0.001 |
LP | 4.70 A | 4.26 bB | 4.24 bB | 4.25 bB | |||||
PS | 4.67 A | 4.70 aA | 4.72 aA | 4.59 aB | |||||
LA | Control | 36.12 D | 53.27 aC | 64.43 aB | 75.44 aA | 0.77 | <0.001 | <0.001 | <0.001 |
LP | 30.05 C | 39.47 bB | 71.91 aA | 69.39 aA | |||||
PS | 33.47 C | 18.07 cD | 51.05 bB | 56.23 bA | |||||
AA | Control | 3.56 B | 3.16 aB | 4.09 bB | 12.06 A | 0.10 | <0.001 | 0.230 | 0.009 |
LP | 3.27 C | 2.39 aC | 5.15 aB | 12.25 A | |||||
PS | 3.44 B | 1.09 bC | 4.61 abB | 12.39 A | |||||
PA | Control | 9.83 AB | 8.31 aB | 9.89 bAB | 12.51 A | 0.20 | <0.001 | 0.008 | <0.001 |
LP | 9.77 B | 5.84 abC | 5.84 cC | 12.40 A | |||||
PS | 9.51 B | 3.83 bC | 12.86 aA | 12.32 A | |||||
BA | Control | ND | ND | ND | ND | - | - | - | - |
LP | ND | ND | ND | ND | |||||
PS | ND | ND | ND | ND | |||||
NH3-N | Control | 22.09 C | 5.60 D | 57.44 aA | 42.53 aB | 0.64 | <0.001 | <0.001 | 0.004 |
LP | 21.80 C | 3.53 D | 41.17 bA | 31.40 bB | |||||
PS | 19.52 C | 5.08 C | 56.44 aA | 41.14 aB | |||||
V-score | Control | 91.31 | 92.71 b | 89.51 b | 90.00 | 0.22 | <0.001 | 0.019 | 0.001 |
LP | 91.50 C | 95.21 abA | 93.09 aB | 90.00 D | |||||
PS | 91.58 B | 97.76 aA | 88.71 bC | 90.00 BC |
Item 1 | Treatment 2 | SEM | p-Value | ||
---|---|---|---|---|---|
Control | LP | PS | |||
Chemical compositions | |||||
Dry matter (g kg−1 FM) | 487.57 | 480.56 | 481.02 | 3.54 | 0.732 |
Crude protein (g kg−1 DM) | 150.40 | 154.20 | 153.60 | 1.06 | 0.331 |
Water soluble carbohydrate (g kg−1 DM) | 9.03 B | 7.03 B | 29.01 A | 3.59 | <0.001 |
Neutral detergent fiber (g/kg (g kg−1 DM) | 366.29 | 347.68 | 342.78 | 8.40 | 0.548 |
Acid detergent fiber (g kg−1 DM) | 231.43 | 222.87 | 218.45 | 6.91 | 0.789 |
In vitro degradability | |||||
DMD (g kg−1) | 579.90 | 606.71 | 608.87 | 6.37 | 0.113 |
NDFD (g kg−1) | 355.29 | 354.55 | 330.79 | 21.84 | 0.902 |
In vitro gas production parameters | |||||
V24h (mL) | 57.15 | 57.01 | 56.57 | 0.72 | 0.957 |
V48h (mL) | 65.68 | 65.10 | 65.04 | 0.80 | 0.951 |
V(∞) (mL) | 64.10 | 63.74 | 63.43 | 0.79 | 0.955 |
k (mL·h−1) | 3.60 | 3.69 | 3.58 | 0.08 | 0.861 |
Item 1 | Treatment 2 | Days of Air Exposure | SEM | p-Value 3 | ||||||
---|---|---|---|---|---|---|---|---|---|---|
0 | 1 | 3 | 5 | 7 | D | T | D × T | |||
Fermentative characteristics | ||||||||||
pH | Control | 4.26 bC | 4.28 bC | 4.28 bC | 4.53 B | 7.52 aA | 0.01 | <0.001 | <0.001 | <0.001 |
LP | 4.25 bC | 4.28 bC | 4.31 bC | 4.56 B | 7.51 aA | |||||
PS | 4.59 a | 4.63 a | 4.65 a | 4.68 | 4.70 b | |||||
LA | Control | 75.44 aA | 71.51 A | 71.55 abA | 71.55 A | 42.46 B | 1.17 | 0.006 | 0.001 | 0.178 |
LP | 69.39 aAB | 67.69 AB | 80.73 aA | 72.89 A | 48.67 B | |||||
PS | 56.23 b | 56.16 | 44.22 b | 51.16 | 50.84 | |||||
AA | Control | 12.06 A | 11.20 A | 9.41 A | 4.80 bB | 2.61 B | 0.35 | <0.001 | 0.172 | 0.224 |
LP | 12.25 A | 10.93 AB | 11.46 A | 5.64 abC | 6.02 BC | |||||
PS | 12.39 A | 11.45 AB | 7.85 C | 8.73 aBC | 7.47 C | |||||
PA | Control | 12.51 | 11.91 | 10.82 | 10.75 | 9.48 b | 0.32 | 0.224 | 0.178 | 0.907 |
LP | 12.41 | 11.20 | 12.63 | 12.30 | 11.19 a | |||||
PS | 12.32 | 11.37 | 9.47 | 9.74 | 9.28 b | |||||
BA | Control | ND | ND | ND | ND | ND | - | - | - | - |
LP | ND | ND | ND | ND | ND | |||||
PS | ND | ND | ND | ND | ND | |||||
NH3-N | Control | 42.53 aB | 47.69 abB | 41.71 B | 44.30 B | 135.85 aA | 2.20 | <0.001 | 0.001 | <0.001 |
LP | 31.40 bB | 39.74 bB | 32.88 B | 53.18 B | 164.96 aA | |||||
PS | 41.14 a | 52.15 a | 44.24 | 41.51 | 44.04 b | |||||
V-score | Control | 90.00 A | 89.84 A | 90.65 A | 89.89 A | 67.90 abB | 0.75 | <0.001 | 0.002 | <0.001 |
LP | 90.00 A | 90.00 A | 90.00 A | 89.37 A | 54.97 bB | |||||
PS | 90.00 | 89.45 | 90.80 | 90.05 | 89.60 a | |||||
Chemical compositions | ||||||||||
DM | Control | 487.57 | 492.51 | 494.73 | 500.26 | 498.86 | 2.33 | 0.466 | 0.058 | 0.918 |
LP | 480.56 | 483.04 | 489.44 | 482.92 | 476.77 | |||||
PS | 481.02 | 489.89 | 499.81 | 499.66 | 504.83 | |||||
CP | Control | 150.40 A | 150.88 A | 152.19 bA | 153.71 A | 144.00 bB | 0.39 | 0.003 | 0.015 | <0.001 |
LP | 154.20 AB | 151.60 B | 157.18 aA | 155.84 AB | 146.07 bC | |||||
PS | 153.60 AB | 154.03 AB | 149.20 bC | 151.04 BC | 155.46 aA | |||||
WSC | Control | 9.03 b | 11.71 b | 8.76 b | 7.92 b | 8.78 c | 0.35 | 0.037 | <0.001 | 0.202 |
LP | 7.03 bC | 8.11 bBC | 6.91 bC | 10.22 bAB | 11.83 bA | |||||
PS | 29.01 a | 32.86 a | 27.18 a | 29.34 a | 30.20 a |
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Xie, Y.; Xu, S.; Li, W.; Wang, M.; Wu, Z.; Bao, J.; Jia, T.; Yu, Z. Effects of the Application of Lactobacillus plantarum Inoculant and Potassium Sorbate on the Fermentation Quality, In Vitro Digestibility and Aerobic Stability of Total Mixed Ration Silage Based on Alfalfa Silage. Animals 2020, 10, 2229. https://doi.org/10.3390/ani10122229
Xie Y, Xu S, Li W, Wang M, Wu Z, Bao J, Jia T, Yu Z. Effects of the Application of Lactobacillus plantarum Inoculant and Potassium Sorbate on the Fermentation Quality, In Vitro Digestibility and Aerobic Stability of Total Mixed Ration Silage Based on Alfalfa Silage. Animals. 2020; 10(12):2229. https://doi.org/10.3390/ani10122229
Chicago/Turabian StyleXie, Yixiao, Shengyang Xu, Wenqi Li, Musen Wang, Zhe Wu, Jinze Bao, Tingting Jia, and Zhu Yu. 2020. "Effects of the Application of Lactobacillus plantarum Inoculant and Potassium Sorbate on the Fermentation Quality, In Vitro Digestibility and Aerobic Stability of Total Mixed Ration Silage Based on Alfalfa Silage" Animals 10, no. 12: 2229. https://doi.org/10.3390/ani10122229