Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Experimental Design and Samples Collection
2.2. LC-MS Metabolomics Analysis
2.3. Measurement of Classical Metabolic Parameters
2.4. Data Processing and Analyses
3. Results
3.1. Plasma Metabolomics Profiling
3.2. Classical Metabolic Parameters
3.3. Correlations Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Items | Normal Body Condition Score (n = 8) | High Body Condition Score (n = 8) | SEM | p-Value |
---|---|---|---|---|
Nonesterified fatty acid, nmol/mL | 162.6 | 171.0 | 12.77 | 0.52 |
β-hydroxybutyrate, µg/mL | 9.97 | 11.56 | 0.916 | 0.10 |
Leptin, pg/mL | 1840.4 | 2078.4 | 116.5 | 0.06 |
Adiponectin, ng/mL | 10.02 | 8.67 | 0.757 | 0.09 |
Tumor necrosis factor –α, pg/mL | 85.2 | 99.4 | 7.58 | 0.08 |
Interleukin 6, pg/mL | 94.6 | 104.0 | 7.12 | 0.21 |
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Wang, J.; Zhang, C.; Zhao, Q.; Li, C.; Jin, S.; Gu, X. Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach. Animals 2020, 10, 1709. https://doi.org/10.3390/ani10091709
Wang J, Zhang C, Zhao Q, Li C, Jin S, Gu X. Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach. Animals. 2020; 10(9):1709. https://doi.org/10.3390/ani10091709
Chicago/Turabian StyleWang, Jian, Chuang Zhang, Qingyao Zhao, Congcong Li, Shuang Jin, and Xianhong Gu. 2020. "Metabolic Profiling of Plasma in Different Calving Body Condition Score Cows Using an Untargeted Liquid Chromatography-Mass Spectrometry Metabolomics Approach" Animals 10, no. 9: 1709. https://doi.org/10.3390/ani10091709