Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Sampling and Transport
2.2. Freeze Immobilization
2.3. Morphological Measures
2.4. Abdomen Dissection
2.5. Spermatheca Extraction and Analysis
2.6. Ovarioles Count
2.7. Statistical Analysis
3. Results and Discussion
3.1. Defects and Descriptive Statistics
3.2. Heritabilities, Genetic and Phenotypic Correlations
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trait | Abbreviation | Unit | N | Mean | SD | CV (%) |
---|---|---|---|---|---|---|
Body weight | bw | mg | 147 | 195.90 | 19.84 | 10.1 |
Head weight | hw | mg | 147 | 13.22 | 1.83 | 13.8 |
Thorax weight | tw | mg | 147 | 77.93 | 8.17 | 10.5 |
Abdomen weight | aw | mg | 147 | 104.76 | 15.28 | 14.6 |
Head width | hwi | mm | 147 | 3.67 | 0.18 | 4.9 |
Thorax width | twi | mm | 147 | 4.67 | 0.26 | 5.6 |
Abdomen width | awi | mm | 147 | 4.80 | 0.21 | 4.4 |
Wing length | wl | mm | 147 | 10.07 | 0.62 | 6.2 |
Spermatheca diameter | sd | mm | 147 | 1.32 | 0.18 | 13.6 |
Spermatheca volume | sv | µl | 147 | 1.27 | 0.53 | 41.7 |
Number of Ovarioles | o | n | 147 | 141 | 25 | 17.9 |
Number of Sperms | sp | n million | 143 | 3.6 | 2.9 | 82.8 |
Trait | Body Weight | Head Weight | Thorax Weight | Abdomen Weight | Head Width | Thorax Width | Abdomen Width | Wing Length | Diameter Spermatheca | Volume Spermatheca | Ovarioles Number | Sperm Count |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Body weight | 0.54 (0.34) | 0.80 (0.31) | 0.92 (0.30) | 0.84 (0.17) | 0.47 (0.50) | 0.34 (0.50) | −0.22 (1.10) | 0.17 (0.62) | 0.23 (0.76) | 0.40 (0.40) | −0.13 (0.50) | −0.13 (0.52) |
Head weight | 0.39 (0.10) | 0.51 (0.35) | 0.99 (0.36) | 0.61 (0.45) | 0.97 (0.46) | 0.34 (0.53) | −0.56 (1.05) | 0.92 (0.71) | −0.44 (0.68) | −0.44 (0.50) | 0.28 (0.50) | 0.23 (0.57) |
Thorax weight | 0.67 (0.06) | 0.19 (0.11) | 0.50 (0.39) | 0.98 (0.69) | −0.01 (0.79) | 0.83 (0.31) | 0.54 (0.75) | 0.74 (0.38) | 0.78 (0.39) | 0.61 (0.41) | −0.18 (0.53) | 0.15 (0.58) |
Abdomen weight | 0.88 (0.03) | 0.31 (0.10) | 0.29 (0.10) | 0.46 (0.34) | 0.47 (0.54) | −0.14 (0.68) | −0.60 (1.19) | −0.45 (0.71) | −0.24 (1.05) | 0.06 (0.54) | −0.15 (0.53) | −0.3 (0.52) |
Head width | 0.36 (0.09) | 0.31 (0.10) | 0.28 (0.10) | 0.27 (0.10) | 0.26 (0.27) | 0.21 (0.67) | 0.15 (1.08) | 0.96 (0.48) | 0.89 (0.48) | 0.7 (0.52) | 0.55 (0.58) | 0.63 (0.56) |
Thorax width | 0.34 (0.10) | 0.30 (0.10) | 0.28 (0.11) | 0.26 (0.10) | 0.32 (0.09) | 0.42 (0.32) | 0.74 (0.36) | 0.98 (0.31) | 0.59 (0.57) | 0.44 (0.43) | 0.02 (0.53) | −0.17 (0.56) |
Abdomen width | 0.39 (0.09) | 0.17 (0.10) | 0.26 (0.10) | 0.33 (0.10) | 0.25 (0.09) | 0.80 (0.04) | 0.13 (0.26) | 0.33 (0.90) | −0.22 (2.07) | 0.44 (0.65) | −0.59 (0.89) | 0.26 (0.96) |
Wing length | 0.34 (0.10) | 0.16 (0.10) | 0.36 (0.10) | 0.20 (0.11) | 0.26 (0.10) | 0.39 (0.09) | 0.30 (0.09) | 0.30 (0.29) | 0.43 (0.81) | 0.40 (0.50) | 0.79 (0.59) | 0.57 (0.52) |
Diameter spermatheca | 0.21 (0.10) | 0.04 (0.12) | 0.18 (0.12) | 0.20 (0.10) | 0.08 (0.12) | 0.15 (0.11) | 0.13 (0.10) | 0.10 (0.10) | 0.17 (0.34) | 0.99 (0.02) | −0.42 (0.64) | −0.96 (0.72) |
Volume spermatheca | 0.22 (0.13) | 0.03 (0.13) | 0.11 (0.12) | 0.18 (0.13) | 0.02 (0.12) | 0.16 (0.13) | 0.16 (0.12) | 0.09 (0.12) | 0.97 (0.01) | 0.88 (0.39) | −0.31 (0.42) | −0.70 (0.56) |
Ovarioles number | 0.01 (0.12) | 0.15 (0.12) | −0.003 (0.12) | −0.02 (0.12) | 0.08 (0.11) | 0.04 (0.12) | 0.03 (0.12) | 0.02 (0.12) | −0.01 (0.12) | −0.05 (0.13) | 0.70 (0.35) | 0.08 (0.48) |
Sperm count | 0.03 (0.12) | 0.03 (0.12) | 0.09 (0.12) | −0.01 (0.12) | 0.10 (0.11) | 0.04 (0.12) | 0.08 (0.11) | 0.17 (0.11) | −0.07 (0.12) | −0.07 (0.13) | 0.05 (0.12) | 0.57 (0.35) |
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Facchini, E.; De Iorio, M.G.; Turri, F.; Pizzi, F.; Laurino, D.; Porporato, M.; Rizzi, R.; Pagnacco, G. Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits. Animals 2021, 11, 3054. https://doi.org/10.3390/ani11113054
Facchini E, De Iorio MG, Turri F, Pizzi F, Laurino D, Porporato M, Rizzi R, Pagnacco G. Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits. Animals. 2021; 11(11):3054. https://doi.org/10.3390/ani11113054
Chicago/Turabian StyleFacchini, Elena, Maria Grazia De Iorio, Federica Turri, Flavia Pizzi, Daniela Laurino, Marco Porporato, Rita Rizzi, and Giulio Pagnacco. 2021. "Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits" Animals 11, no. 11: 3054. https://doi.org/10.3390/ani11113054