Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Origin of Chicken Samples
2.2. Protein and RNA Purification from Chicken Caecal and Ileal Tissue
2.3. Protein Mass Spectrometry
2.4. Quantitative Reverse-Transcribed PCR (qRT-PCR)
2.5. Statistics
2.6. Ethics Approval and Consent to Participate
3. Results
3.1. Gross Comparison of Protein Expression in the Ileum and Caecum
3.2. Ileum-Specific Proteins
3.3. Caecum-Specific Proteins
3.4. Functional Analysis of Caecum-Specific Proteins
3.5. Expression of Key Regulatory Proteins of Oxidative Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Protein Annotation | Protein | p-Value | Abundance in Ileum [%] | DFR * | qRT-PCR Fold |
---|---|---|---|---|---|
ENSGALP00000010239 | ACTA2 | 1.73 × 10−15 | 4.94 | 4.67 | -# |
ENSGALP00000039176 | ACTB | 2.09 × 10−17 | 4.82 | 6.75 | NS |
ENSGALP00000018042 | ACTC | 2.09 × 10−17 | 4.64 | 4.87 | - |
ENSGALP00000002196 | FABP6 | 6.95 × 10−8 | 4.12 | 311.34 | 2.43 |
ENSGALP00000037398 | VIL1 | 4.18 × 10−17 | 0.60 | 5.21 | 6.43 |
ENSGALP00000019528 | FABP2 | 7.73 × 10−16 | 0.50 | 13.89 | - |
ENSGALP00000015467 | SI | 1.78 × 10−14 | 0.40 | 20.94 | 313.41 |
ENSGALP00000003847 | CTNNA1 | 7.72 × 10−16 | 0.27 | 3.30 | NS |
ENSGALP00000037173 | SCIN | 1.33 × 10−13 | 0.26 | 5.21 | - |
ENSGALP00000005520 | ALDH9A1 | 5.55 × 10−15 | 0.2 | 3.90 | - |
ENSGALP00000020953 | SERPINB6 | 3.45 × 10−16 | 0.17 | 3.61 | - |
ENSGALP00000000256 | MGAM | 5.94 × 10−5 | 0.16 | 11.77 | - |
ENSGALP00000007819 | PEPD | 1.48 × 10−12 | 0.13 | 4.42 | - |
ENSGALP00000032098 | BAAT | 1.85 × 10−7 | 0.12 | 12.42 | 7.00 |
ENSGALP00000035815 | SLC25A6 | 2.67 × 10−13 | 0.12 | 3.42 | - |
ENSGALP00000008315 | ANPEP | 1.68 × 10−14 | 0.10 | 6.76 | - |
ENSGALP00000013682 | GSTO1 | 9.86 × 10−14 | 0.10 | 3.85 | - |
ENSGALP00000022884 | PLA2G2E | 3.05 × 10−8 | 0.09 | 7.81 | - |
ENSGALP00000026341 | ANXA13 | 5.55 × 10−16 | 0.09 | 4.58 | - |
ENSGALP00000036834 | MTTP | 3.05 × 10−5 | 0.09 | 13.57 | - |
Group Description | No. of Proteins | % Expression out of Ileum-Specific Proteins | Proteins |
---|---|---|---|
Actin and actin-binding | 7 | 70.13 | ACTA2; ACTB; ACTC; VIL1; CTNNA1; SCIN; MYO1A |
Bile metabolism and transport | 2 | 19.04 | FABP6; BAAT |
Digestion enzymes | 4 | 3.53 | SI; MGAM; PEPD; ANPEP |
Lipid metabolism and transport | 4 | 3.51 | FABP2; PLA2G2E; MTTP; PLIN4 |
Other | 8 | 3.80 | SERPINB6; ANXA13; GSTO1; SLC25A6; RPS8; RPS8; GDA; SORD |
Protein Annotation | Protein | p-Value | Abundance in Caecum [%] | DFR * | qRT-PCR Fold | Influenced by Microbiota Colonisation # |
---|---|---|---|---|---|---|
ENSGALP00000020275 | GAL-1 | 7.49 × 10−11 | 0.63 | 7.83 | 2.40 | |
ENSGALP00000025280 | TXN | 7.89 × 10−13 | 0.59 | 5.62 | 6.39 | UP |
ENSGALP00000001060 | c-factor-like | 8.37 × 10−17 | 0.48 | 6.20 | - | |
ENSGALP00000026062 | CBS | NA | 0.43 | 500.00 | 3488.35 | UP |
ENSGALP00000001274 | SELENBP1 | 8.72 × 10−17 | 0.36 | 6.44 | 14.72 | |
ENSGALP00000019979 | ADH1C | 1.73 × 10−15 | 0.21 | 7.24 | 31.09 | DOWN |
ENSGALP00000036423 | TUBB2B | NA | 0.20 | 500.00 | - | |
ENSGALP00000004816 | PRDX6 | 7.23 × 10−13 | 0.20 | 3.28 | 13.14 | |
ENSGALP00000029947 | TST | 3.65 × 10−11 | 0.19 | 4.47 | 4.90 | UP |
ENSGALP00000017869 | NID1 | 5.04 × 10−12 | 0.18 | 3.81 | - | |
ENSGALP00000005480 | AKR1B1L | NA | 0.18 | 500.00 | - | DOWN |
ENSGALP00000005607 | PRELP | 9.41 × 10−16 | 0.18 | 4.56 | - | |
ENSGALP00000024396 | ALDH1A1 | 1.58 × 10−12 | 0.17 | 7.02 | 16.55 | DOWN |
ENSGALP00000005654 | FN1 | 2.98 × 10−12 | 0.16 | 5.50 | 2.37 | |
ENSGALP00000020214 | NID2 | 2.37 × 10−14 | 0.14 | 4.12 | - | |
ENSGALP00000020373 | MPST | 8.72 × 10−17 | 0.14 | 6.46 | 7.43 | |
ENSGALP00000023289 | AHNAK | NA | 0.12 | 500.00 | 2.19 | |
ENSGALP00000039757 | HYOU1 | 2.52 × 10−9 | 0.12 | 4.13 | - | |
ENSGALP00000015277 | CAPZA2 | 4.18 × 10−17 | 0.11 | 5.11 | - | |
ENSGALP00000018939 | GC | 4.16 × 10−9 | 0.11 | 3.68 | - | DOWN |
Group Description | No. of Proteins | % Expression out of Caecum-Specific Proteins | Proteins |
---|---|---|---|
Drug metabolic processes and metabolism of exogenous substances | 12 | 22.48 | AKR1B1L, ALDH1A1, PRDX6, MPST, CBS, ADH1C, SELENBP1, SULT1E1, NDUFV2, FH, NDUFS1, ENO2 |
Wound healing | 12 | 17.39 | GAL-1, FN1, AHNAK, CAPZA2, FHL2, CAPZA1, PLG, TNC, NMNAT3, AIMP1, COL5A1, POSTN |
Extracellular matrix organisation and cell–matrix adhesion proteins | 14 | 13.52 | FN1, NID2, NID1, PRELP, TNC, PLG, COL5A1, POSTN, AIMP1, CAST, TNXB, PXN, CTTN, ACTN2 |
Cellular response to stress | 8 | 11.59 | TXN, TUBB2B, PRDX6, CAPZA2, CAPZA1, ATOX1, ST13, TNXB |
H2S metabolism | 4 | 8.71 | CBS, MPST, TST, SELENBP1 |
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Volf, J.; Rajova, J.; Babak, V.; Seidlerova, Z.; Rychlik, I. Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken. Animals 2021, 11, 3155. https://doi.org/10.3390/ani11113155
Volf J, Rajova J, Babak V, Seidlerova Z, Rychlik I. Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken. Animals. 2021; 11(11):3155. https://doi.org/10.3390/ani11113155
Chicago/Turabian StyleVolf, Jiri, Jana Rajova, Vladimir Babak, Zuzana Seidlerova, and Ivan Rychlik. 2021. "Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken" Animals 11, no. 11: 3155. https://doi.org/10.3390/ani11113155