Phytogenic Water Additives Improve Broiler Growth Performance via Modulation of Intermediary Metabolism-Related Signaling Pathways
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Animal Husbandry and Experimental Design
2.3. RNA Isolation and RT-qPCR
2.4. Western Blot Analysis
2.5. Statistical Analysis
3. Results
3.1. Hepatic Lipogenesis-Related Pathways
3.2. Lipolysis-Related Genes and Proteins in Adipose Tissue
3.3. Protein Synthesis-Related Pathway in Breast Muscle
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameters b | Experimental Groups c | |||||
---|---|---|---|---|---|---|
Control | AVSSL | |||||
SCD-1 | 1.00 | ± | 0.21 | 1.82 | ± | 0.30 * |
SREBP1 | 1.00 | ± | 0.11 | 0.79 | ± | 0.10 |
SREBP2 | 1.00 | ± | 0.26 | 0.46 | ± | 0.05 * |
SCAP | 1.00 | ± | 0.21 | 0.29 | ± | 0.03 * |
PPARα | 1.00 | ± | 0.10 | 1.04 | ± | 0.10 |
PPARγ | 1.00 | ± | 0.14 | 1.00 | ± | 0.09 |
INSIG2 | 1.00 | ± | 0.14 | 1.00 | ± | 0.14 |
CD36 | 1.00 | ± | 0.19 | 0.86 | ± | 0.18 |
AdipoQ | 1.00 | ± | 0.17 | 0.61 | ± | 0.08 * |
AdipoR1 | 1.00 | ± | 0.11 | 1.01 | ± | 0.11 |
AdipoR2 | 1.00 | ± | 0.12 | 1.19 | ± | 0.13 |
NAMPT | 1.00 | ± | 0.11 | 1.30 | ± | 0.15 |
Parameters b | Experimental Groups c | |||||
---|---|---|---|---|---|---|
Control | SG | |||||
SCD-1 | 1.00 | ± | 0.21 | 1.84 | ± | 0.39 * |
SREBP1 | 1.00 | ± | 0.11 | 0.80 | ± | 0.08 |
SREBP2 | 1.00 | ± | 0.26 | 0.55 | ± | 0.08 |
SCAP | 1.00 | ± | 0.21 | 0.28 | ± | 0.04 * |
PPARα | 1.00 | ± | 0.10 | 1.16 | ± | 0.15 |
PPARγ | 1.00 | ± | 0.14 | 1.96 | ± | 0.53 |
INSIG2 | 1.00 | ± | 0.14 | 0.90 | ± | 0.16 |
CD36 | 1.00 | ± | 0.19 | 0.72 | ± | 0.15 |
AdipoQ | 1.00 | ± | 0.17 | 1.48 | ± | 0.34 |
AdipoR1 | 1.00 | ± | 0.11 | 1.52 | ± | 0.21 * |
AdipoR2 | 1.00 | ± | 0.12 | 1.30 | ± | 0.11 * |
NAMPT | 1.00 | ± | 0.11 | 1.43 | ± | 0.25 |
Parameters b | Experimental Groups c | |||||
---|---|---|---|---|---|---|
Control | AVSSL | |||||
AMPKα1 | 1.00 | ± | 0.27 | 0.97 | ± | 0.10 |
AMPKα2 | 1.00 | ± | 0.19 | 0.79 | ± | 0.19 |
AMPKβ1 | 1.00 | ± | 0.05 | 1.66 | ± | 0.20 |
AMPKβ2 | 1.00 | ± | 0.10 | 0.77 | ± | 0.14 |
AMPKγ1 | 1.00 | ± | 0.06 | 0.94 | ± | 0.11 |
AMPKγ2 | 1.00 | ± | 0.18 | 1.45 | ± | 0.21 |
AMPKγ3 | 1.00 | ± | 0.09 | 0.88 | ± | 0.20 |
Parameters b | Experimental Groups c | |||||
---|---|---|---|---|---|---|
Control | SG | |||||
AMPKα1 | 1.00 | ± | 0.27 | 0.93 | ± | 0.31 |
AMPKα2 | 1.00 | ± | 0.19 | 0.93 | ± | 0.15 |
AMPKβ1 | 1.00 | ± | 0.05 | 1.08 | ± | 0.16 |
AMPKβ2 | 1.00 | ± | 0.10 | 0.81 | ± | 0.08 |
AMPKγ1 | 1.00 | ± | 0.06 | 0.89 | ± | 0.05 |
AMPKγ2 | 1.00 | ± | 0.18 | 1.10 | ± | 0.13 |
AMPKγ3 | 1.00 | ± | 0.09 | 0.90 | ± | 0.14 |
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Flees, J.J.; Emami, N.K.; Greene, E.; Ganguly, B.; Dridi, S. Phytogenic Water Additives Improve Broiler Growth Performance via Modulation of Intermediary Metabolism-Related Signaling Pathways. Animals 2021, 11, 750. https://doi.org/10.3390/ani11030750
Flees JJ, Emami NK, Greene E, Ganguly B, Dridi S. Phytogenic Water Additives Improve Broiler Growth Performance via Modulation of Intermediary Metabolism-Related Signaling Pathways. Animals. 2021; 11(3):750. https://doi.org/10.3390/ani11030750
Chicago/Turabian StyleFlees, Joshua J., Nima K. Emami, Elizabeth Greene, Bhaskar Ganguly, and Sami Dridi. 2021. "Phytogenic Water Additives Improve Broiler Growth Performance via Modulation of Intermediary Metabolism-Related Signaling Pathways" Animals 11, no. 3: 750. https://doi.org/10.3390/ani11030750