Conjugative Plasmid-Mediated Extended Spectrum Cephalosporin Resistance in Genetically Diverse Escherichia coli from a Chicken Slaughterhouse
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. Isolation and Detection
2.3. Molecular Characterization
2.4. Conjugation Assay and Molecular Characterization of the Transconjugants
3. Results
3.1. Distribution of ESBL/AmpC Genes in E. coli Isolates from the Slaughterhouse and Retail Meat
3.2. Occurrence of ESBL/AmpC genes in E. coli Isolates in the Slaughterhouse Environment
3.3. Correlation between ESBL/AmpC-Producing E. coli from the Slaughterhouse and Retail Meat
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Phenotype | No. of Isolates | Conjugation Result (No.) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Resistance Genes | Total No. | Slaughterhouse | Retail Meat | Resistance Genes Transfer | Total No. | Slaughterhouse | Retail Meat | |||
Lairage | Slaughterhouse Environment | Lairage | Slaughterhouse Environment | |||||||
ESBL | NA | 3 | 3 | |||||||
TEM-1 | 5 | 5 | ||||||||
CTX-M-1 | 6 | 5 | 1 | CTX-M-1 | 6 | 5 | 1 | |||
CTX-M-14 | 7 | 6 | 1 | CTX-M-14 | 7 | 6 | 1 | |||
CTX-M-15 | 2 | 2 | ||||||||
CTX-M-55 | 2 | 1 | 1 | |||||||
CTX-M-65 | 1 | 1 | ||||||||
TEM-1, CTX-M-1 | 8 | 4 | 3 | 1 | CTX-M-1 | 8 | 4 | 3 | 1 | |
TEM-1, CTX-M-14 | 2 | 2 | CTX-M-14 | 2 | 2 | |||||
TEM-1, CTX-M-55 | 4 | 2 | 2 | TEM-1, CTX-M-55 | 4 | 2 | 2 | |||
AmpC | CMY-2 | 24 | 8 | 12 | 4 | CMY-2 | 14 | 4 | 8 | 2 |
TEM-1, CMY-2 | 16 | 12 | 4 | CMY-2 | 8 | 6 | 2 | |||
TEM-135, CMY-2 | 1 | 1 | CMY-2 | 1 | 1 |
Flocks No. (Date) | Typing | Slaughterhouse Processing a | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
1. Lairage | 2. Scalding | 3. Feather Removal | 4. First-Convey | 5. Evisceration | 8. Second-Washing | 10. Third-Convey | 11. Third-Washing | 12. Handling Workers | ||
1 (160405) | PFGE type | 11, 20, 29, 37, 49, 56 | 9 | 1, 22 | 22 | 52 | ||||
Conjugative plasmids | NA | NA | NA | blaCMY-2 IncI1-ST86 | blaCMY-2 IncI1-ST12 | |||||
2 (160407) | PFGE type | 23, 30, 45, 60, 63 | 2 | |||||||
Conjugative plasmids | NA, blaCTX-M-1IncI1-ST87 | blaCMY-2 IncI1-ST12 | ||||||||
3 (160412) | PFGE type | 5, 17 | 54 | 40 | 27 | |||||
Conjugative plasmids | blaCTX-M-1 IncI1-ST87 | blaCMY-2 IncI1-ST18 | blaTEM-1, CTX-M-55 IncFIB | blaCTX-M-1 IncI1-ST87 | ||||||
4 (160414) | PFGE type | 6, 19, 35, 48, 51 | 54 | 54 | 54 | |||||
Conjugative plasmids | blaCMY-2 IncI1-ST12 | blaCMY-2 IncI1-ST12 | blaCMY-2 IncI1-ST12 | blaCMY-2 IncI1-ST12 | ||||||
blaCTX-M-1 IncI1-ST87 | ||||||||||
5 (160419) | PFGE type | 32 | 32 | 25 | ||||||
Conjugative plasmids | blaCTX-M-1 IncI1-ST87 | NA | blaCTX-M-1 IncI1-ST87 | |||||||
6 (160428) | PFGE type | 13, 16 | 21, 22 | 33 | ||||||
Conjugative plasmids | blaCMY-2 IncI1-ST108 | NA, blaCMY-2 IncI1-ST12 | blaTEM-1, CTX-M-55 IncFIB |
Flock No. | Lairage a | Slaughter-Line | Retail Meat | |||
---|---|---|---|---|---|---|
PFGE Type | Conjugative Plasmid | PFGE Type | Conjugative Plasmid | PFGE Type | Conjugative Plasmid | |
1 | 11, 20, 29, 37, 49, 56 | NA | 1, 9, 22, 52 | blaCMY-2 IncI1-ST86 | nd | |
blaCMY-2 IncI1-ST12 | nd | |||||
2 | 23, 30, 45, 60, 63 | NA, blaCTX-M-1 IncI1-ST87 | 2 | blaCMY-2 IncI1-ST12 | nd | |
3 | 5, 17 | blaCTX-M-1 IncI1-ST87 | 27, 40, 54 | blaCMY-2 IncI1-ST18 | nd | |
blaTEM-1, CTX-M-55 IncFIB | nd | |||||
blaCTX-M-1 IncI1-ST87 | nd | |||||
4 | 6, 19, 35, 48, 51 | blaCMY-2 IncI1-ST12 | 54 | blaCMY-2 IncI1-ST12 | nd | |
blaCTX-M-1 IncI1-ST87 | nd | |||||
5 | - | - | 25, 32 | blaCTX-M-1 IncI1-ST87 | nd | |
6 | 13, 16 | blaCMY-2 IncI1-ST108 | 21, 22, 33 | blaCMY-2 IncI1-ST108 | nd | |
blaCMY-2 IncI1-ST12 | nd | |||||
blaTEM-1, CTX-M-55 IncFIB | nd | |||||
7 | 38, 53, 58, 59, 62 | blaCMY-2 IncI1-ST12 | nd | - | - | |
8 | - | - | nd | 4 | blaCTX-M-1 IncI1-ST87 | |
9 | 8, 10, 42 | blaCTX-M-14 IncI1-ST162 | nd | - | - | |
10 | - | - | nd | 36 | blaTEM-1, CTX-M-55 IncN | |
11 | 7, 41, 55 | NA | nd | nd | nd | |
12 | - | - | nd | 34 | blaTEM-1, CTX-M-55 IncN | |
13 | nd | nd | nd | 43 | blaCTX-M-1 IncI1-ST87 | |
14 | - | - | nd | 58 | blaCMY-2 IncI1-ST12 | |
15 | 3, 15 | blaCTX-M-14 IncI1-ST38 | nd | 15 | blaCMY-2 IncI1-ST12 | |
16 | nd | nd | nd | 62 | NA | |
17 | - | - | nd | 50 | blaCTX-M-14 IncI1-ST87 | |
18 | 31, 47 | blaCMY-2 IncI1-ST86 | nd | - | - | |
19 | 26, 57 | NA | nd | 22, 24 | NA | |
20 | 18, 31 | blaCTX-M-14 IncI1-ST162 | nd | - | - | |
21 | - | - | nd | 61 | blaCTX-M-14 IncI1-ST162 | |
22 | - | - | nd | 25 | blaCMY-2 IncI1-ST12 | |
23 | 10, 12, 39 | NA, blaCTX-M-1 IncI1-ST38 | nd | 44 | blaCTX-M-14 IncI1-ST38 | |
24 | 28 | blaCMY-2 IncI1-ST12 | nd | - | - | |
25 | 14, 46 | blaCTX-M-14 IncI1-ST87 | nd | - | - |
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Wei, B.; Shang, K.; Cha, S.-Y.; Zhang, J.-F.; Jang, H.-K.; Kang, M. Conjugative Plasmid-Mediated Extended Spectrum Cephalosporin Resistance in Genetically Diverse Escherichia coli from a Chicken Slaughterhouse. Animals 2021, 11, 2491. https://doi.org/10.3390/ani11092491
Wei B, Shang K, Cha S-Y, Zhang J-F, Jang H-K, Kang M. Conjugative Plasmid-Mediated Extended Spectrum Cephalosporin Resistance in Genetically Diverse Escherichia coli from a Chicken Slaughterhouse. Animals. 2021; 11(9):2491. https://doi.org/10.3390/ani11092491
Chicago/Turabian StyleWei, Bai, Ke Shang, Se-Yeoun Cha, Jun-Feng Zhang, Hyung-Kwan Jang, and Min Kang. 2021. "Conjugative Plasmid-Mediated Extended Spectrum Cephalosporin Resistance in Genetically Diverse Escherichia coli from a Chicken Slaughterhouse" Animals 11, no. 9: 2491. https://doi.org/10.3390/ani11092491
APA StyleWei, B., Shang, K., Cha, S. -Y., Zhang, J. -F., Jang, H. -K., & Kang, M. (2021). Conjugative Plasmid-Mediated Extended Spectrum Cephalosporin Resistance in Genetically Diverse Escherichia coli from a Chicken Slaughterhouse. Animals, 11(9), 2491. https://doi.org/10.3390/ani11092491