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Article

Exposure of Free-Ranging Wild Animals to Zoonotic Leptospira interrogans Sensu Stricto in Slovenia

by
Diana Žele-Vengušt
1,
Renata Lindtner-Knific
2,
Nina Mlakar-Hrženjak
2,
Klemen Jerina
3 and
Gorazd Vengušt
1,*
1
Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
2
Institute of Poultry, Birds, Small Mammals and Reptiles, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
3
Department of Forestry and Renewable Forest Resources, Biotechnical Faculty, Večna pot 83, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Animals 2021, 11(9), 2722; https://doi.org/10.3390/ani11092722
Submission received: 24 July 2021 / Revised: 10 September 2021 / Accepted: 14 September 2021 / Published: 17 September 2021
(This article belongs to the Special Issue Zoonoses and Wildlife: One Health Approach)
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Abstract

:

Simple Summary

Wildlife can serve as a reservoir for highly contagious and deadly diseases, many of which are infectious to domestic animals and/or humans. Wildlife pathogen and disease surveillance is, thus, an essential tool that can provide valuable information on population health status and protect human health. Blood samples from 244 wild animals and 5 from carcasses were tested for specific antibodies against Leptospira serovars in Slovenia between 2019 and 2020 using the microscopic agglutination test. The results confirm that various wildlife species were exposed to Leptospira interrogans and may be used as a sentinel for leptospirosis, which is considered a significant health threat to other wildlife species and to humans.

Abstract

A total of 249 serum samples from 13 wild animal species namely fallow deer (Dama dama, n = 1), roe deer (Capreolus capreolus, n = 80), red deer (Cervus elaphus, n = 22), chamois (Rupicapra rupicapra, n = 21), mouflon (Ovis musimon, n = 4), brown hare (Lepus europaeus, n = 2), nutria (Myocastor coypus, n = 1), red fox (Vulpes vulpes, n = 97), stone marten (Martes foina, n = 12), European badger (Meles meles, n = 2), golden jackal (Canis aureus, n = 2) Eurasian lynx (Lynx lynx, n = 2) and grey wolf (Canis lupus, n = 3) were analysed for the presence of antibodies against Leptospira interrogans sensu stricto. Serum samples were examined via the microscopic agglutination test for the presence of specific antibodies against Leptospira serovars Icterohaemorrhagiae, Bratislava, Pomona, Grippotyphosa, Hardjo, Sejroe, Australis, Autumnalis, Canicola, Saxkoebing and Tarassovi. Antibodies to at least one of the pathogenic serovars were detected in 77 (30.9%; CI = 25–37%) sera. The proportion of positive samples varied intraspecifically and was the biggest in large carnivores (lynx, wolf and jackal; 86%), followed by mezzo predators: stone marten (67%) and red fox (34%), and large herbivores: red deer (32%), roe deer (25%), alpine chamois (10%) and mouflon (0%). Out of the 77 positive samples, 42 samples (53.8%) had positive titres against a single serovar, while 35 (45.4%) samples had positive titres against two or more serovars. The most frequently detected antibodies were those against the serovar Icterohaemorrhagiae. The present study confirmed the presence of multiple pathogenic serovars in wildlife throughout Slovenia. It can be concluded that wild animals are reservoirs for at least some of the leptospiral serovars and are a potential source of leptospirosis for other wild and domestic animals, as well as for humans.

1. Introduction

In recent decades, international attention on wildlife diseases, including surveillance and monitoring programmes, has increased [1,2]. Wildlife diseases occur in numerous forms in a wide range of species and populations around the globe. Leptospirosis is a zoonosis of global importance, affecting many species of wild and domestic animals, as well as humans [1,3]. Leptospira spp. are also considered as small mammal-associated zoonotic pathogens causing diseases with potentially similar symptoms in humans [4]. It is considered as one of the most important re-emerging health threats to humans by the World Organisation for Animal Health [5,6]. Various pathogenic serovars of Leptospira have been serologically classified into 22 serogroups and over 300 serovars based on the microscopic agglutination test (MAT) or the cross-agglutination absorption test (CAAT), respectively [7], with each serovar tending to be maintained by a host group and capable of causing the disease [8,9,10]. Small rodents are the usual reservoirs of leptospires in natural herds [11,12,13]. Data from the Netherlands show that insectivores and rodents could serve as indicators of environmental contamination and/or wildlife contamination with Leptospira spp. [14]. Studies worldwide indicate that various wild ruminants [15,16,17,18], lagomorphs [15,18] and carnivores [15,19,20,21] are also potential sources of leptospires. Wildlife species are generally considered to be important epidemiological vectors, mainly because of their frequent reactivity to Leptospira serovars native to their habitat [16]. These reservoirs are thought to act as a source of infection for humans and domestic animals, who can then become a source of infection for other animals and humans [6,11]. However, data on the epidemiology of Leptospira infections in wildlife and the public health significance of wildlife species worldwide are lacking [20,22].
The presence of antibodies in wild animals may indicate previous or current infection, which may have occurred either by direct contact with the contaminated urine of another animal or by the consumption of infected prey [23]. Several domestic and wild animals become infected and, thus, become kidney carriers, excreting the pathogen through their urine [23] and via parent–offspring transmission [24]. Wildlife are reservoir hosts for leptospires and often show no clinical signs of disease; however, these reservoirs can serve as a source of infection for humans and domestic animals, who can then become a source of infection for other animals and humans [5,6,25]. In addition, there are few data on Leptospira antibodies and infections in wildlife, although transmission to livestock and humans often originates from or is maintained by wildlife [25].
Humans may be exposed to Leptospira infections directly through contact with infected material or indirectly through the contaminated environment [26,27]. Studies have shown that peak incidence of disease occurred after periods of excessive rainfall and flooding [28]. Infections with Leptospira spp. can affect not only people in exposed professions (i.e., veterinarians, trappers, abattoir workers, farm workers, hunters, animal shelter workers and scientists and technicians involved with animals in laboratories or in the field) but also people who work with marine mammals, fishmen, researchers, wildlife rehabilitators, animal trainers and zoological park workers [3]. A study conducted in Austria [29] reported that hunters in particular are exposed to zoonotic agents, including leptospires, probably through the direct contact of abraded skin or mucous membranes with the tissues, blood or urine of infected animals [30,31]. In some cases, leptospirosis can present as a severe disease in both animals and humans and can lead to death [11]. Human infections by Leptospira spp. and orthohantaviruses are almost indistinguishable in their clinical presentation [32] and can often be confused with each other [4]. In addition, coinfections with orthohantaviruses are frequently observed at sites where the prevalence of Leptospira spp. in small mammals exceeded 35% [4]. Therefore, long-term active surveillance and studies of wildlife reservoirs will help to understand the role of wildlife as a reservoir and as a source of leptospires and other pathogens to humans [13]. According to Podgoršek [33], the epidemiological picture in Slovenia is comparable to that in Europe. Up to 30 cases of Leptospira infection with the predominant serovars Grippotyphosa and Icterohaemorrhagiae and the species L. kirschneri and L. interrogans sensu stricto are reported annually [33,34]. In Slovenia, the risk of contracting leptospirosis is associated with occupational and recreational exposure [34].
MAT is considered the gold standard for sero-diagnosis of leptospirosis because of its unsurpassed diagnostic specificity [35]; however it is not sufficiently sensitive for the diagnosis of the acute phase of the disease [36]. It would be an important tool for epidemiological purposes, such as identifying infecting serovars [36]. Antigens can be detected by histological, histochemical or immunostaining techniques. Unfortunately, none of these tests are currently suitable for routine laboratory use because of technical limitations and low sensitivity [37]. Isolation of Leptospira from the clinical specimen is difficult because leptospires are fastidious, slow growing and require special growth media, and it is time consuming and laborious [38]. Therefore, PCR assay is very useful as a contemporary method for diagnosis in the acute phase of leptospirosis [39].
To date, studies in Slovenia have confirmed the presence of specific antibodies against 11 pathogenic Leptospira serovars in wild boar [40], but there is currently no information on the seroprevalence and distribution of leptospirosis in other wildlife species in Slovenia. The aim of this study was to investigate the seroprevalence of pathogenic L. interrogans serovars in different wild species in Slovenia.

2. Materials and Methods

2.1. Samples

During the 2019 and 2020 hunting season (May to December), blood samples were collected nationwide from a total of 244 apparently healthy, free-ranging wild animals, and 5 clotted blood samples were collected from carcasses (Table 1 and Figure 1). Licensed game wardens and hunters were invited to submit samples from animals shot during the regular annual cull or from animals found dead in nature. Prior to sample collection, hunters were instructed on procedures and were provided with field sample kits. Immediately after animal death, blood samples were collected from the jugular vein or heart. As a part of the national passive health surveillance of wildlife in Slovenia, carcasses of wild large predators (Eurasian lynx and grey wolf) found dead in their habitats were sent for necropsy to the Veterinary Faculty, University of Ljubljana. Clotted blood samples were taken from the heart. The authors declare that no animals were killed for the purpose of this study and that all procedures contributing to this work met the ethical standards of the relevant national and European regulations on the care and use of animals (Directive 2010/63/EC).

2.2. Laboratory Methods

After field collection, blood samples were transported to the Veterinary Faculty, University of Ljubljana, within 24 h. Many of the collected samples were haemolysed and were, therefore, rejected at the pre-analysis stage (n = 34). Fresh blood samples and clotted blood samples were centrifuged at 4000 rpm for 15 min (LC 320) to obtain the sera. Sera were transferred with serum pipettes into sterile Eppendorf tubes and stored at −20 °C before being tested for the presence of specific antibodies against pathogenic serovars of Leptospira interrogans sensu stricto using the MAT.
Live cultures of different serovars were used as antigens: Grippotyphosa, strain Moskva V; Sejroe, strain Mallerdorf 84; Pomona, strain Pomona; Tarassovi, strain Mitis Johnson; Copenhageni (serological group: Icterohaemorrhagiae), strain Wijnberg; Canicola, strain Hond Utrecht IV; Australis, strain Ballico; Autumnalis, strain Akyami A; Bataviae, strain Van Tienen; Saxkoebing, strain MUS 24; Bratislava, strain Jež Bratislava; and Hardjo, strain Hardjo Bovis. The MAT was performed according to the accredited method in accordance with the protocol standard operating procedure (SOP 120) in the laboratory for leptospirosis at the Veterinary Faculty in Ljubljana and was carried out in two phases. In the first phase (pretest), the presence of specific antibodies for the serovars used in the test was determined, while in the second phase, a twofold titration of positive sera, starting with the dilution 1:50, was performed. Phosphate buffer (PBS; Dulbecco’s phosphate-buffered saline, Sigma-Aldrich, Burlington, MA, USA) was used for serum dilutions. Results were read using a darkfield microscope with a magnification of 160, and the endpoint was estimated as 50% agglutination or the lysis of leptospires in the microscopic field. Samples that had titres of ≥50 against one or more serovars were considered positive.

2.3. Statistical Analyses

Estimates and confidence intervals (CIs, for p = 0.05) of the estimated proportions of individuals exposed to L. interrogans for each of the species studied were calculated, taking into account the binomial distribution of the exposure outcome (yes/no). Confidence intervals were estimated only for the species with adequate sample size. Differences in the extent of exposure to L. interrogans among the animal species studied were also investigated. These differences were evaluated with chi-square tests of homogeneity, first for all species together, and in the second phase, for all combinations of pairs of species. Test of homogeneity provides reliable results if the theoretical frequency in each cross-section of the levels of tested variables is larger than 1. To reach this condition, species with very small sample size (n < 4) were either excluded from analysis (fallow deer, badger, hare and nutria) or, in the case of species with similar biology and thus expected similar prevalence for examined disease, data from several species were pulled together in a wider group, e.g., species Eurasian lynx, grey wolf and golden jackal were joined in new group named “large carnivores”.

3. Results

Examination of 249 blood sera from wild animals revealed antibodies to at least one of the pathogenic serovars in 77 sera (30.9%; CI 25.2–36.7%) (Table 2 and Figure 1). Of the 77 positive samples, 42 samples (53.8%) had positive titres against a single serovar, while 35 (45.4%) samples had positive titres against two or more serovars. Of all positive reactions, the highest antibody seroprevalence was found for serovar Australis in red fox and stone marten; serovar Icterohaemorrhagiae in golden jackal, grey wolf and roe deer; serovar Pomona in red deer; serovars Icterohaemorrhagiae and Sejroe in Eurasian lynx; serovars Icterohaemorrhagiae and Tarassovi in chamois; and serovar Bratislava in nutria. Serovar Icterohaemorrhagiae showed the highest antibody titre, 1:6400 in red fox (Table 3). No antibodies were detected in fallow deer, European badger, European mouflon or brown hare.
A test of homogeneity shows that there are differences in the proportion of positive cases between different animal species (Pearson chi-square = 25.6; df = 7; p = 0.00059). Overall, the largest proportion of positive cases was detected in the large carnivores group (86%; CI = 60–100%; n = 7), followed by two medium-sized predator species: stone marten (67%; CI = 40–93%; n = 12) and red fox (34% CI = 25–43%; n = 97), and in declining order the following four species of large herbivores: roe deer (25%; CI = 16–34%; n = 80), red deer (32%; CI = 12–51%; n = 22), alpine chamois (10%; CI = 0–22%; n = 21) and as the last European mouflon with no detected positive cases (0%; n = 4). Other species had too low a sample size for statistical analysis. The single analysed sample of nutria was positive and the sample of fallow deer was negative for the presence of specific antibodies against Leptospira serovars; moreover, both results of both tested samples of brown hare and European badgers were negative.
Paired comparisons between species proved differences between large carnivores and different species of large herbivores, between stone marten and species of large herbivores, and between red fox and alpine chamois (p < 0.05), but it is noteworthy that outcomes of the formal statistical test depend on sample size and are prone to type I error in cases of small samples (Table 4).

4. Discussion

Wildlife and domestic animals play an important role as the reservoir for particular Leptospira serovars. Environmental characteristics, topography, meteorology, human presence and species interactions can influence the occurrence and density of Leptospira species [41]. In wild animals, specific climatic, edaphic and hydrological factors also determine the incidence of leptospirosis in different habitats [42]. Differences in prevalence and serovars between studies in wildlife could be due to inconsistencies in cutoff titres, serovars tested, sampling site characteristics, climate or geographic location and timing of the year of the study [43].
In this study, blood samples were taken at random from animals shot during the regular annual cull or found dead in the wild without any knowledge of possible infection or its duration. Determination of antibody titre by MAT has been used as a tool for leptospirosis diagnosis. Different diagnostic tests that can be used to detect leptospirosis have advantages and disadvantages, and laboratory diagnosis of leptospirosis is challenging. A positive culture of biological samples (blood, urine, tissue) is the definitive proof of infection, but culturing leptospires is laborious and fastidious. The bacterium requires special growth media, and incubation can last for months [44]. Histopathological examination of the kidneys is not indicated to replace the serological diagnosis of leptospirosis, and may be used only as a complementary examination [45]. In the early stages of the disease, the only sensitive and specific test is PCR. Its limitation is that it does not detect DNA in the blood during the first 5–10 days after the onset of the disease and until the 15th day [37]. MAT is the most commonly used serological test in the diagnosis of leptospirosis, despite being a technically demanding and laborious procedure [46]. MAT can be positive from the 10th to 12th day after disease onset and can detect both class M and class G antibodies [37]. MAT has a sensitivity of 41% in the first week, 82% in the second to fourth week and 96% after the fourth week of illness [47]. MAT is considered the gold standard for serodiagnosis of leptospirosis due to its unsurpassed diagnostic specificity [35], but it is not sufficiently sensitive for diagnosis of the acute phase of the disease [36]. Another limitation of serology is that it cannot distinguish between current, recent or previous infections [37]. In the present study, paired samples to confirm acute or convalescent infection were not available.
The analyses performed in the present study showed that most of the carnivores studied (with the exception of the European badger) and the most abundant wild ruminants in Slovenia were frequently exposed to L. interrogans. The significant percentage of seropositive red fox, stone marten, grey wolf and other carnivores tested may indicate that those species are reliable sentinels for epidemiological monitoring in Slovene forest habitats, which can also explain positive titres to L. interrogans in roe deer and red deer sharing the same biotope. Infection by multiple serogroups was confirmed, suggesting that multiple epidemiological cycles exist in the Slovenian region. The results of our study confirmed antibodies against 10 pathogenic Leptospira serovars in carnivores, 8 in wild ruminants and 1 in nutria. The observed seroprevalence of leptospiral antibodies in the tested wildlife species could not be extrapolated to the whole population level in Slovenia due to the statistically insufficient number of samples but could be a good indicator of the importance of these wildlife species in leptospirosis transmission.
The red fox (Vulpes vulpes) is widely distributed in European countries [48] and is the most widespread mesopredator in Slovenia. Since 2013, the hunting bag of red fox increased from 10.400 to 15.715 in 2019 [49,50]. Several serological studies using MAT have shown that red foxes are frequently exposed to different serovars of Leptospira spp. [19,21,51,52,53]. The results of our study confirm that interactions between different Leptospira serovars are also common in the Slovenian red fox population. The seroprevalence of antibodies to leptospiral serovars (34%) found in this study was lower than the seroprevalence reported in red foxes from Spain (47.1%) [19] but higher than that in other European countries, such as Poland (26.3%) [21], Croatia (31.25%) [33], Norway (9.9%) [51] and Germany (1.9%) [52], all using MAT. The different results from the different countries are difficult to explain because research on the seroprevalence of Leptospira spp. in foxes requires extensive ecological knowledge of fox population dynamics and must include considerations of juvenile fall migrations, home range, population density, litter size, yearly accession, mortality rate and hunting pressure [53]. It is also very important to consider epidemiological data on leptospiral archaic foci, reservoirs, maintenance hosts and serovar distribution [42]. Antibodies against serovar Australis (51.5%) were detected most frequently, followed by those against serovar Bratislava (48.5%) and Icterohaemorrhagiae (33.3%). Antibodies against serovar Australis were also the most frequently detected antibodies in red foxes in Croatia [54]. According to data from studies in other European countries, the most common serovar in red foxes is Icterohaemorrhagiae [19,51], while the Bratislava serovar is less common in Europe. The exposure of foxes to this serovar is not surprising because rodents, an important food source for foxes, are probably the most important host for a variety of Leptospira serovars in rural and urban environments [55,56].
Among all European members of the marten family, the stone marten is the only species whose population is increasing, and it is one of the most widespread mustelids in the Eurasian region [57]. The current population size is unknown. The results of our study confirmed the presence of specific antibodies against various serovars of Leptospira in the stone marten population. Leptospira antibodies were found in eight animals, a seroprevalence of 66.6% (8/12). The high seroprevalence in stone martens in Slovenia is comparable to that in Croatia (4/7; 62.50%) [42], while in Spain and France, all samples (n = 8) were negative against L. interrogans serovars using MAT [18,58]. In Slovenia, antibodies against serovar Australis (66.66%) were most frequently detected in stone martens, followed by those against serovar Bratislava (50%) and Icterohaemorrhagiae (25%).
The golden jackal (Canis aureus) is one of the most widespread canid species [59] and has also established territories in Slovenia [60]. A rough estimate of the population size in Slovenia is about 1000 individuals [61]. The results of our study confirmed the presence of specific antibodies against different serovars of Leptospira in the golden jackal population. Leptospira antibodies were found in two animals with a seroprevalence of 100% (2/2). The study data indicated that golden jackals could transmit different Leptospira serovars. They showed titres against Icterohaemorrhagiae, Pomona, Hardjo, Sejroe and Saxkoebing serovars. The seroprevalence of antibodies against leptospiral serovars found in this study was comparable to the seroprevalence reported in golden jackals from Ukraine (100%; 9/9). Both golden jackals tested had titres against five serovars [62].
The grey wolf (Canis lupus) is the largest wild member of the dog family (Canidae). Its population in Slovenia is increasing and includes over 100 individuals [63]. Leptospira antibodies were found in two animals with a seroprevalence of 66.6% (2/3). Serological reactions for the Grippotyphosa, Pomona and Icterohaemorrhagiae serogroups were detected in a grey wolf in Italy [64]. Few studies have been conducted in the United States of America, where seroprevalence in grey wolves ranged from 1% to 11% [65,66], and the most commonly detected serovar was Grippotyphosa.
The Eurasian lynx (Lynx lynx) is the third-largest predator in Europe after the brown bear and grey wolf. The population in Slovenia is estimated at only about 15 individuals [67]. Leptospira antibodies were found in two animals with a seroprevalence of 100% (2/2). The study data in Eurasian lynx showed low titres of antibodies against serovars Icterohaemorrhagiae and Sejroe. Seroprevalence in Iberian lynx in Spain [19] and Quebec in wild lynx (Lynx canadensis) was 32% (7/22) and 1% (1/97), respectively. In Spain, the most frequently detected serovars were Icterohaemorrhagiae, and in Quebec, in one case, Pomona and Bratislava. According to Labelle et al. [68], the low seroprevalence of antibodies to L. interrogans in lynx is unexpected because rodents, one of the main food sources for these animals, are known reservoirs of L. interrogans. Throughout Europe and in Slovenia, the diet of lynx usually consists of European roe deer, which is clearly the preferred prey of Eurasian lynx [69]. In this study, the seroprevalence of antibodies to L. interrogans in roe deer was 25%. We believe that roe deer may also serve as a source of leptospirosis for lynx.
The European roe deer (Capreolus capreolus) is the most common and widespread deer species in Europe [70]. The rough estimate of roe deer population size in Slovenia is about 110,000 individuals [71]. In recent decades, the population size and, at the same time, the hunting bag of roe deer have greatly increased in most parts of Europe [72]. Roe deer is one of the most important game species and a crucial prey of large carnivores in Europe [70,73]. In roe deer, a seroprevalence of 25% (20/80) was observed. Animals showed titres against five serovars. The majority of the positive samples had positive titres against a single serovar. Antibodies against serovar Icterohaemorrhagiae (65%) were most frequently detected. The seroprevalence of antibodies against leptospiral serovars found in this study was considerably higher than that in Croatia (6.0%) [42] or in Poland, which showed an overall seroprevalence in deer (roe deer, red deer and fallow deer) of 4.8% [74], or in Germany (2%) [75]. No positive serological reactions were found in roe deer (n = 66) in Italy [17].
The red deer (Cervus elaphus) is the second most abundant deer species in almost all of Europe [76]. The rough estimate of the population size is 10,000–14,000 animals [77]. A seroprevalence of 31.8% (7/22) was found in red deer. The animals showed titres against seven serovars. Antibodies against serovar Pomona (42.8%) were detected most frequently. The seroprevalence of antibodies against leptospiral serovars found in this study was significantly higher than that in Italy (6.33%) [17] or in Poland, which showed a total seroprevalence in deer (roe deer, red deer and fallow deer) of 4.8% [74], or in Croatia (19.02%) [42].
Chamois (Rupicapra rupicapra) is a habitat-specialised ungulate inhabiting “continental archipelagos” with fragmented rocky habitats, often restricted to high altitudes [78]. The estimated number of chamois in Slovenia is 10,000 [77]. Leptospira antibodies were found in two animals, a seroprevalence of 9.52% (2/21). Study data revealed that chamois had antibodies against serovars Icterohaemorrhagiae and Tarassovi. In Italy, no positive serological reactions for Leptospira serovars were found in chamois (n = 138) [17]. To our knowledge, our study is the first to report positive samples in chamois for Leptospira antibodies. It is, however, noteworthy that the proportion of positive samples in chamois was among the lowest between all analysed species in Slovenia being followed only by mouflon (0/4).
The coypu, also known as the nutria (Myocastor coypus), is a semiaquatic rodent and significant carrier of pathogenic Leptospira in Europe [79]. The current population size is unknown. We tested one animal and detected antibodies against serovar Bratislava. Several researchers in Europe have presented information on antibodies to leptospiral serovars in nutria, ranging from 11.5% in Italy [80] to 76% in France [81] with the predominance of the Icterohaemorrhagiae serogroup. These results are consistent with the idea that nutria should be considered a risk factor for leptospirosis in humans and domestic animals and should be taken into account by public health decision makers, especially with regard to prevention and population control [79].
Unlike clinical disease seen in canines and humans, the health impact of leptospirosis in wildlife is unclear [43]. Necropsy of fresh carcasses of animals killed in traffic accidents to look for renal lesions [19] and collection of tissue for immunohistochemistry would help in confirming the disease [82].
The risk of contracting leptospirosis is associated with occupational and recreational hazards [34]. Strategies for leptospirosis prevention are, therefore, based on education about the epidemiology and transmission mechanisms of leptospirosis [83]. Education of occupationally exposed workers about contact with contaminated water or infected animals is particularly important. Personal protective measures should also be taken for workers in high-risk occupations. The risk of infection can be reduced by increasing awareness of the routes of infection, avoiding contact with high risk water sources and using of prophylaxis during high-risk activities [84]. Increased efforts should be made to identify and treat infected animals at an early stage and to raise awareness of immunisation options for domestic and farm animals [83].

5. Conclusions

Our data confirm that large and medium-sized carnivores are frequently exposed to the pathogenic serovars of L. interrogans and may play the role of sentinel for leptospirosis. Data on L. interrogans-specific antibody-positive wild ruminants suggest that these species, although less infected, may still be a potential source of leptospirosis for humans, with the risk of infection particularly high for veterinarians, butchers, people working in forested areas and, frequently overlooked, hunting dogs. Due to the small number of samples tested, further investigation of the prevalence of infection in wild animals in Slovenia is needed to clarify the epidemiological significance of wild animals for leptospirosis transmission.

Author Contributions

Conceptualisation, D.Ž.V., G.V. and R.L.-K.; methodology, D.Ž.V., K.J., R.L.-K. and G.V.; software, K.J. and R.L.-K.; validation, R.L.-K. and N.M.-H.; formal analysis, R.L.-K., K.J. and N.M.-H.; investigation, G.V., R.L.-K., N.M.-H. and D.Ž.V.; data curation, G.V.; writing—original draft preparation, G.V. and D.Ž.V.; writing—review and editing, D.Ž.V., R.L.-K., N.M.-H., K.J. and G.V.; visualisation, D.Ž.V., G.V. and R.L.-K.; supervision, G.V. and R.L.-K.; funding acquisition, G.V., R.L.-K. and D.Ž.V. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Slovenian Research Agency (research core funding No. P4-0092), the Administration of the Republic of Slovenia for Food Safety, Veterinary Sector and Plant Protection and the Slovenian Hunting Association.

Institutional Review Board Statement

No ethical approval was required for the sample types collected in this study. Animals were harvested by hunters either during the regular annual cull or when they were taken from the wild due to disease as decided by the game warden. The authors declare that no animals were killed for the purpose of this study and that all procedures that contributed to this work meet the ethical standards of the relevant national and European regulations on the care and use of animals (Directive 2010/63/EC).

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available upon request from the corresponding author.

Acknowledgments

The authors thank all hunters for their participation in the study through collecting samples. We would also like to thank the Administration of the Republic of Slovenia for Food Safety, Veterinary Service and Plant Protection; the Slovenian Research Agency and the Hunting Association of Slovenia for supporting the research.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Mörner, T.; Obendorf, D.L.; Artois, M.; Woodford, M.H. Surveillance and monitoring of wildlife diseases. Rev. Sci. Tech. 2002, 21, 67–76. [Google Scholar] [CrossRef]
  2. Guberti, V.; Stancampiano, L.; Ferrari, N. Surveillance, monitoring and surveys of wildlife diseases: A public health and conservation approach. Hystrix. It J. Mamm 2014, 3–8. [Google Scholar]
  3. Cilia, G.; Bertelloni, F.; Albini, S.; Fratini, F. Insight into the Epidemiology of Leptospirosis: A Review of. Animals 2021, 11, 191. [Google Scholar] [CrossRef]
  4. Jeske, K.; Jacob, J.; Drewes, S.; Pfeffer, M.; Heckel, G.; Ulrich, R.G.; Imholt, C. Hantavirus-Leptospira coinfections in small mammals from central Germany. Epidemiol. Infect. 2021, 149, e97. [Google Scholar] [CrossRef]
  5. Bharti, A.R.; Nally, J.E.; Ricaldi, J.N.; Matthias, M.A.; Diaz, M.M.; Lovett, M.A.; Levett, P.N.; Gilman, R.H.; Willig, M.R.; Gotuzzo, E.; et al. Leptospirosis: A zoonotic disease of global importance. Lancet Infect. Dis. 2003, 3, 757–771. [Google Scholar] [CrossRef]
  6. Bengis, R.G.; Leighton, F.A.; Fischer, J.R.; Artois, M.; Mörner, T.; Tate, C.M. The role of wildlife in emerging and re-emerging zoonoses. Rev. Sci. Tech. 2004, 23, 497–511. [Google Scholar] [PubMed]
  7. Picardeau, M. Virulence of the zoonotic agent of leptospirosis: Still terra incognita? Nat. Rev. Microbiol. 2017, 15, 297–307. [Google Scholar] [CrossRef]
  8. Adler, B.; Peña, D.L.; Moctezuma, A. Leptospira. In Pathogenesis of Bacterial Infections of Animals; Gyles, C., Prescott, J., Songer, G., Thoen, C., Eds.; Blackwell Publishing: Hoboken, NJ, USA, 2004; pp. 385–396. [Google Scholar]
  9. Vincent, A.T.; Schiettekatte, O.; Goarant, C.; Neela, V.K.; Bernet, E.; Thibeaux, R.; Ismail, N.; Mohd Khalid, M.K.N.; Amran, F.; Masuzawa, T.; et al. Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Negl. Trop. Dis. 2019, 13, e0007270. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  10. Guglielmini, J.; Bourhy, P.; Schiettekatte, O.; Zinini, F.; Brisse, S.; Picardeau, M. Genus-wide Leptospira core genome multilocus sequence typing for strain taxonomy and global surveillance. PLoS Negl. Trop. Dis. 2019, 13, e0007374. [Google Scholar] [CrossRef] [PubMed]
  11. Levett, P.N. Leptospirosis. Clin. Microbiol. Rev. 2001, 14, 296–326. [Google Scholar] [CrossRef] [Green Version]
  12. Cvetnic, Z.; Margaletic, J.; Toncic, J.; Turk, N.; Milas, Z.; Spicic, S.; Lojkic, M.; Terzic, S.; Jemersic, L.; Humski, A.; et al. A serological survey and isolation of leptospires from small rodents and wild boars in the Republic of Croatia. Vet. Med. Czech. 2003, 11, 321–329. [Google Scholar] [CrossRef] [Green Version]
  13. Zhang, C.; Xu, J.; Zhang, T.; Qiu, H.; Li, Z.; Zhang, E.; Li, S.; Chang, Y.F.; Guo, X.; Jiang, X.; et al. Genetic characteristics of pathogenic Leptospira in wild small animals and livestock in Jiangxi Province, China, 2002-2015. PLoS Negl. Trop. Dis. 2019, 13, e0007513. [Google Scholar] [CrossRef] [PubMed]
  14. Krijger, I.M.; Ahmed, A.A.A.; Goris, M.G.A.; Cornelissen, J.B.W.J.; Groot Koerkamp, P.W.G.; Meerburg, B.G. Wild rodents and insectivores as carriers of pathogenic Leptospira and Toxoplasma gondii in The Netherlands. Vet. Med. Sci 2020, 6, 623–630. [Google Scholar] [CrossRef] [PubMed]
  15. Hartskeerl, R.A.; Terpstra, W.J. Leptospirosis in wild animals. Vet. Q. 1996, 18 (Suppl. 3), S149–S150. [Google Scholar] [CrossRef]
  16. Espí, A.; Prieto, J.M.; Alzaga, V. Leptospiral antibodies in Iberian red deer (Cervus elaphus hispanicus), fallow deer (Dama dama) and European wild boar (Sus scrofa) in Asturias, Northern Spain. Vet. J. 2010, 183, 226–227. [Google Scholar] [CrossRef] [Green Version]
  17. Andreoli, E.; Radaelli, E.; Bertoletti, I.; Bianchi, A.; Scanziani, E.; Tagliabue, S.; Mattiello, S. Leptospira spp. infection in wild ruminants: A survey in Central Italian Alps. Vet. Ital. 2014, 50, 285–291. [Google Scholar] [CrossRef]
  18. Ayral, F.; Djelouadji, Z.; Raton, V.; Zilber, A.L.; Gasqui, P.; Faure, E.; Baurier, F.; Vourc’h, G.; Kodjo, A.; Combes, B. Hedgehogs and Mustelid Species: Major Carriers of Pathogenic Leptospira, a Survey in 28 Animal Species in France (20122015). PLoS ONE 2016, 11, e0162549. [Google Scholar] [CrossRef]
  19. Millán, J.; Candela, M.G.; López-Bao, J.V.; Pereira, M.; Jiménez, M.A.; León-Vizcaíno, L. Leptospirosis in wild and domestic carnivores in natural areas in Andalusia, Spain. Vector Borne Zoonotic Dis. 2009, 9, 549–554. [Google Scholar] [CrossRef]
  20. Fornazari, F.; Langoni, H.; Marson, P.M.; Nóbrega, D.B.; Teixeira, C.R. Leptospira reservoirs among wildlife in Brazil: Beyond rodents. Acta Trop. 2018, 178, 205–212. [Google Scholar] [CrossRef] [Green Version]
  21. Żmudzki, J.; Arent, Z.; Jabłoński, A.; Nowak, A.; Zębek, S.; Stolarek, A.; Bocian, Ł.; Brzana, A.; Pejsak, Z. Seroprevalence of 12 serovars of pathogenic Leptospira in red foxes (Vulpes vulpes) in Poland. Acta Vet. Scand. 2018, 60, 34. [Google Scholar] [CrossRef] [Green Version]
  22. Vieira, A.S.; Pinto, P.S.; Lilenbaum, W. A systematic review of leptospirosis on wild animals in Latin America. Trop. Anim. Health Prod. 2018, 50, 229–238. [Google Scholar] [CrossRef]
  23. Sharma, S.; Vijayachari, P.; Sugunan, A.P.; Sehgal, S.C. Leptospiral carrier state and seroprevalence among animal population—A cross-sectional sample survey in Andaman and Nicobar Islands. Epidemiol. Infect. 2003, 131, 985–989. [Google Scholar] [CrossRef]
  24. Mwachui, M.A.; Crump, L.; Hartskeerl, R.; Zinsstag, J.; Hattendorf, J. Environmental and Behavioural Determinants of Leptospirosis Transmission: A Systematic Review. PLoS Negl. Trop. Dis. 2015, 9, e0003843. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Cirone, S.M.; Riemann, H.P.; Ruppanner, R.; Behymer, D.E.; Franti, C.E. Evaluation of the hemagglutination test for epidemiologic studies of leptospiral antibodies in wild mammals. J. Wildl. Dis. 1978, 14, 193–202. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Bierque, E.; Thibeaux, R.; Girault, D.; Soupé-Gilbert, M.E.; Goarant, C. A systematic review of Leptospira in water and soil environments. PLoS ONE 2020, 15, e0227055. [Google Scholar] [CrossRef] [PubMed]
  27. Haake, D.A.; Dundoo, M.; Cader, R.; Kubak, B.M.; Hartskeerl, R.A.; Sejvar, J.J.; Ashford, D.A. Leptospirosis, water sports, and chemoprophylaxis. Clin. Infect. Dis. 2002, 34, e40–e43. [Google Scholar] [CrossRef]
  28. Lau, C.L.; Smythe, L.D.; Craig, S.B.; Weinstein, P. Climate change, flooding, urbanisation and leptospirosis: Fuelling the fire? Trans. R Soc. Trop. Med. Hyg. 2010, 104, 631–638. [Google Scholar] [CrossRef]
  29. Deutz, A.; Fuchs, K.; Schuller, W.; Müller, M.; Kerbl, U.; Klement, C. Studies on the seroseroprevalence of antibodies against Leptospira interrogans in hunters and wild boar from south-eastern Austria. Z. Jagdwiss 2002, 48, 60–65. [Google Scholar]
  30. Mason, R.J.; Fleming, P.J.; Smythe, L.D.; Dohnt, M.F.; Norris, M.A.; Symonds, M.L. Leptospira interrogans antibodies in feral pigs from New South Wales. J. Wildl. Dis. 1998, 34, 738–743. [Google Scholar] [CrossRef] [Green Version]
  31. Ko, A.I.; Goarant, C.; Picardeau, M. Leptospira: The dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat. Rev. Microbiol. 2009, 7, 736–747. [Google Scholar] [CrossRef]
  32. Sunil-Chandra, N.P.; Clement, J.; Maes, P.; De Silva, H.J.; Van Esbroeck, M.; Van Ranst, M. Concomitant leptospirosis-hantavirus co-infection in acute patients hospitalized in Sri Lanka: Implications for a potentially worldwide underestimated problem. Epidemiol. Infect. 2015, 143, 2081–2093. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Podgoršek, D. Phenotypic and Genotypic Identification of Leptospira spp. and Evaluation of Microbiological Methods for Diagnosis of Infection; Biotehnical Faculty: Ljubljana, Slovenija, 2017. [Google Scholar]
  34. Bedernjak, J. Leptospiroza pri nas in v Svetu (Leptospirosis in Our Country and in the World); Bedernjak, J., Ed.; Pomurska Založba: Murska Sobota, Slovenija, 1993; p. 136. [Google Scholar]
  35. Goris, M.G.; Hartskeerl, R.A. Leptospirosis serodiagnosis by the microscopic agglutination test. Curr. Protoc. Microbiol. 2014, 32, Unit 12E.15. [Google Scholar] [CrossRef] [PubMed]
  36. Niloofa, R.; Fernando, N.; de Silva, N.L.; Karunanayake, L.; Wickramasinghe, H.; Dikmadugoda, N.; Premawansa, G.; Wickramasinghe, R.; de Silva, H.J.; Premawansa, S.; et al. Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test. PLoS ONE 2015, 10, e0129236. [Google Scholar] [CrossRef] [Green Version]
  37. Musso, D.; La Scola, B. Laboratory diagnosis of leptospirosis: A challenge. J. Microbiol. Immunol. Infect. 2013, 46, 245–252. [Google Scholar] [CrossRef] [Green Version]
  38. Katz, A.R. Quantitative polymerase chain reaction: Filling the gap for early leptospirosis diagnosis. Clin. Infect. Dis. 2012, 54, 1256–1258. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Mullan, S.; Panwala, T.H. Polymerase Chain Reaction: An Important Tool for Early Diagnosis of Leptospirosis Cases. J. Clin. Diagn Res. 2016, 10, DC08–DC11. [Google Scholar] [CrossRef]
  40. Vengušt, G.; Lindtner-Knific, R.; Žele, D.; Bidovec, A. Leptospira antibodies in wild boars (Sus scrofa) in Slovenia. Eur. J. Wildl. Res. 2008, 54, 749–752. [Google Scholar] [CrossRef]
  41. Soberon, J.; Peterson, A.T. Interpretation of Models of Fundamental Ecological Niches and Species’ Distributional Areas. Biodivers Inform. 2005, 2, 1–10. [Google Scholar] [CrossRef] [Green Version]
  42. Slavica, A.; Cvetnić, Ž.; Milas, Z.; Janicki, Z.; Turk, N.; Konjevi, D.; Severin, K.; Tončić, J.; Lipej, Z. Incidence of leptospiral antibodies in different game species over a 10-year period (1996–2005) in Croatia. Eur. J. Wildl. 2008, 54, 305–311. [Google Scholar] [CrossRef]
  43. Grimm, K.; Rivera, N.A.; Fredebaugh-Siller, S.; Weng, H.Y.; Warner, R.E.; Maddox, C.W.; Mateus-Pinilla, N.E. Evidence of Leptospira Serovars in Wildlife and Leptospiral DNA in Water Sources in a Natural Area in East Central Illinois, USA. J. Wildl. Dis. 2020, 56, 316–327. [Google Scholar] [CrossRef]
  44. Di Azevedo, M.I.N.; Pires, B.C.; Libonati, H.; Pinto, P.S.; Cardoso Barbosa, L.F.; Carvalho-Costa, F.A.; Lilenbaum, W. Extra-renal bovine leptospirosis: Molecular characterization of the Leptospira interrogans Sejroe serogroup on the uterus of non-pregnant cows. Vet. Microbiol. 2020, 250, 108869. [Google Scholar] [CrossRef]
  45. Magalhães, G.M.; Alvarenga, P.B.D.; Medeiros-Ronchi, A.A.; Moreira, T.d.A.; Gundim, L.F.; Gomes, D.O.; Lima, A.M.C. Leptospirosis in slaughtered cows in the Triangulo Mineiro, Minas Gerais: Prevalence, serological profile and renal lesions. Biosci. J. 2020, 36, 539–545. [Google Scholar] [CrossRef] [Green Version]
  46. Levett, P.N. Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis. Clin. Infect. Dis. 2003, 36, 447–452. [Google Scholar] [CrossRef] [PubMed]
  47. Sehgal, S.C.; Vijayachari, P.; Sharma, S.; Sugunan, A.P. LEPTO Dipstick: A rapid and simple method for serodiagnosis of acute leptospirosis. Trans. R. Soc. Trop. Med. Hyg. 1999, 93, 161–164. [Google Scholar] [CrossRef]
  48. Eckert, J.; Conraths, F.J.; Tackmann, K. Echinococcosis: An emerging or re-emerging zoonosis? Int. J. Parasitol. 2000, 30, 1283–1294. [Google Scholar] [CrossRef] [PubMed]
  49. Slovenia-Forest-Service. Slovenia Forest Service Report on Forest for Year 2019. Available online: www.zgs.si/zavod/publikacije/letna_porocila/index.html (accessed on 7 June 2021).
  50. Slovenia-Forest-Service. Slovenia Forest Service Report on Forest for Year 2013. Available online: www.zgs.si/zavod/publikacije/letna_porocila/index.html (accessed on 7 June 2021).
  51. Akerstedt, J.; Lillehaug, A.; Larsen, I.L.; Eide, N.E.; Arnemo, J.M.; Handeland, K. Serosurvey for canine distemper virus, canine adenovirus, Leptospira interrogans, and Toxoplasma gondii in free-ranging canids in Scandinavia and Svalbard. J. Wildl. Dis. 2010, 46, 474–480. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Müller, H.; Winkler, P. [Results of serological studies of Leptospira antibodies in foxes]. Berl. Munch Tierarztl. Wochenschr. 1994, 107, 90–93. [Google Scholar]
  53. Milas, Z.; Turk, N.; Janicki, Z.; Slavica, A.; Starešina, V.; Barbić, L.J.; Lojkić, M.; Modrić, Z. Leptospiral antibodies in red foxes (Vulpes vulpes) in northwest Croatia. Vet. Arhiv. 2006, 76, 51–57. [Google Scholar]
  54. Slavica, A.; Dezdek, D.; Konjevic, D.; Cvetnic, Z.; Sindicic, M.; Stanin, D.; Habus, J.; Turk, N. Prevalence of leptospiral antibodies in the red fox (Vulpes vulpes) population of Croatia. Vet. Med. (Praha) 2011, 56, 209–213. [Google Scholar]
  55. Dupouey, J.; Faucher, B.; Edouard, S.; Richet, H.; Kodjo, A.; Drancourt, M.; Davoust, B. Human leptospirosis: An emerging risk in Europe? Comp. Immunol. Microbiol. Infect. Dis. 2014, 37, 77–83. [Google Scholar] [CrossRef] [PubMed]
  56. Boey, K.; Shiokawa, K.; Rajeev, S. Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS Negl. Trop. Dis. 2019, 13, e0007499. [Google Scholar] [CrossRef]
  57. Genovesi, P.; Secchi, M.; Boitani, L. Diet of stone martens: An example of ecological flexibility. J. Zool 1996, 238, 545–555. [Google Scholar] [CrossRef]
  58. Millán, J.; Velarde, R.; Chirife, A.D.; León-Vizcaíno, L. Carriage of pathogenic Leptospira in carnivores at the wild/domestic interface. Pol. J. Vet. Sci. 2019, 22, 589–598. [Google Scholar] [CrossRef] [PubMed]
  59. Arnold, J.; Humer, A.; Heltai, M.; Murariu, D.; Spassov, N.; Hackländer, K. Current status and distribution of golden jackals Canis aureus in Europe. Mamm. Rev. 2011, 42, 1–11. [Google Scholar] [CrossRef]
  60. Krofel, M. Confirmed presence of territorial groups of golden jackals (Canis aureus) in Slovenia. Nat. Slov. 2009, 11, 65–68. [Google Scholar]
  61. Potočnik, H.; Pokorny, B.; Flajšman, K.; Kos, I. Evrazijski Šakal (Eurasian Jackal); Leskovic, B., Ed.; Lovska zveza Slovenije: Ljubljana, Slovenia, 2019; p. 248. [Google Scholar]
  62. Nakonechnyi, I.V.; Perots’ka, L.V.; Pyvovarova, I.V.; Chornyi, V.A. Ecological and epizootic roles of Golden jackal, genus Canis aureus in the Northwest of Black Sea coast. Sci. Messenger LNU Vet. Med. Biotechnol. 2019, 21, 37–43. [Google Scholar] [CrossRef]
  63. Simčič, G.; Fležar, U.B.; Bartol, M.; Černe, R.; Berce, T.; Krofel, M.; Potočnik, H.; Jelenčič, M.; Kuralt, Ž. Spremljanje varstvenega stanja volkov v Sloveniji v letih 2018 in 2019 (Monitoring of wolf population in Slovenija in seasons 2018 and 2019). Lovec 2020, 103, 328–331. [Google Scholar]
  64. Bregoli, M.; Pesaro, S.; Ustulin, M.; Vio, D.; Beraldo, P.; Galeotti, M.; Cocchi, M.; Lucchese, L.; Bertasio, C.; Boniotti, M.B.; et al. Environmental Exposure of Wild Carnivores to Zoonotic Pathogens. Int. J. Environ. Res. Public Health 2021, 18, 2512. [Google Scholar] [CrossRef]
  65. Zarnke, R.L.; Ballard, W.B. Serologic survey for selected microbial pathogens of wolves in Alaska, 1975-1982. J. Wildl. Dis. 1987, 23, 77–85. [Google Scholar] [CrossRef] [Green Version]
  66. Khan, M.A.; Goyal, S.M.; Diesch, S.L.; Mech, L.D.; Fritts, S.H. Seroepidemiology of leptospirosis in Minnesota wolves. J. Wildl. Dis 1991, 27, 248–253. [Google Scholar] [CrossRef] [Green Version]
  67. Žerjav, S.; Černe, R.; Stergar, M.; Krofel, M.; Majić Skrbinšek, A.; Skrbinšek, T.; Berce, T.; Bartol, M.; Potočnik, H.; Kos, I.; et al. Project LIFE Lynx - Preventing the Extinction of the Dinaric-SE Alpine Lynx Population. Lovec 2017, 9, 428–429. [Google Scholar]
  68. Labelle, P.; Mikaelian, I.; Martineau, D.; Beaudin, S.; Blanchette, N.; Lafond, R.; St-Onge, S. Seroprevalence of leptospirosis in lynx and bobcats from Quebec. Can. Vet. J. 2000, 41, 319–320. [Google Scholar]
  69. Krofel, M.; Huber, D.; Kos, I. Diet of Eurasian lynx Lynx lynx in the northern Dinaric Mountains (Slovenia and Croatia). Acta Theriol. 2011, 56, 315–322. [Google Scholar] [CrossRef]
  70. Melis, C.; Nilsen, E.B.; Panzacchi, M.; Linnell, J.D.; Odden, J. Roe deer face competing risks between predators along a gradient in abundance. Ecosphere 2013, 4, 1–12. [Google Scholar] [CrossRef] [Green Version]
  71. Jerina, K.; Stergar, M.; Jelenko, I.; Pokorny, B. Spatial Distibution, Fitness, and Population Dynamics of Ungulates in Slovenia: Studies on the Effects of Spatially Explicite Habitat and Species-Specific Factors and Predicting Future Trends; Biotechnical Faculty of the University of Ljubljana: Ljubljana, Slovenia, 2010; p. 48. [Google Scholar]
  72. Burbaitė, L.; Csányi, S. Roe deer population and harvest changes in Europe. Estonian J. Ecol 2009, 58, 169–180. [Google Scholar] [CrossRef]
  73. Linnell, J.D.C.; Zachos, F. Status and distribution patterns of European ungulates: Genetics, population history and conservation. In Ungulate Management in Europe—Problems and Practices; Putman, R., Apollonio, M., Andersen, R., Eds.; Cambridge University Press: Cambridge, UK, 2011; pp. 12–53. [Google Scholar]
  74. Zmudzki, J.; Jablonski, A.; Arent, Z.; Zebek, S.; Nowak, A.; Stolarek, A. Parzeniecka-Jaworska, M. First report of Leptospira infections in red deer, roe deer, and fallow deer in Poland. J. Vet. 2016, 60, 257–260. [Google Scholar] [CrossRef] [Green Version]
  75. Witt, W.; Dedek, J.; Loepelmann, H. Occurrence of Leptospira antibodies in red, roe and fallow deer and mouflon. Monatsh Veterinarmed 1988, 43, 65–68. [Google Scholar]
  76. Burbaité, L.; Csányi, S. Red deer population and harvest changes in Europe. Acta Zool Lit. 2010, 20, 179–188. [Google Scholar] [CrossRef] [Green Version]
  77. Adamič, M.; Jerina, K. Ungulates and their management in Slovenia. In European Ungulates and Their Management in the 21st Century; Apollonio, M., Andersen, R., Putman, R., Eds.; Cambridge University Press: Cambridge, UK, 2010; pp. 507–527. [Google Scholar]
  78. Bužan, E.; Bryja, J.; Zemanová, B.; Kryštufek, B. Population genetics of chamois in the contact zone between the Alps and the Dinaric mountains: Uncovering the role of habitat fragmentation and past management. Cons Genet. 2013, 14, 401–412. [Google Scholar] [CrossRef]
  79. Ayral, F.; Kodjo, A.; Guédon, G.; Boué, F.; Richomme, C. Muskrats are greater carriers of pathogenic Leptospira than coypus in ecosystems with temperate climates. PLoS ONE 2020, 15, e0228577. [Google Scholar] [CrossRef] [Green Version]
  80. Bollo, E.; Pregel, P.; Gennero, S.; Pizzoni, E.; Rosati, S.; Nebbia, P.; Biolatti, B. Health status of a population of nutria (Myocastor coypus) living in a protected area in Italy. Res. Vet. Sci. 2003, 75, 21–25. [Google Scholar] [CrossRef]
  81. Vein, J.; Leblond, A.; Belli, P.; Kodjo, A.; Berny, P.J. The role of the coypu (Myocastor coypus), an invasive aquatic rodent species, in the epidemiological cycle of leptospirosis: A study in two wetlands in the East of France. Eur. J. Wildl. Res. 2014, 60, 125–133. [Google Scholar] [CrossRef]
  82. Shearer, K.E.; Harte, M.J.; Ojkic, D.; Delay, J.; Campbell, D. Detection of Leptospira spp. in wildlife reservoir hosts in Ontario through comparison of immunohistochemical and polymerase chain reaction genotyping methods. Can. Vet. J. 2014, 55, 240–248. [Google Scholar] [PubMed]
  83. Poeppl, W.; Orola, M.J.; Herkner, H.; Müller, M.; Tobudic, S.; Faas, A.; Mooseder, G.; Allerberger, F.; Burgmann, H. High prevalence of antibodies against Leptospira spp. in male Austrian adults: A cross-sectional survey, April to June 2009. Euro Surveill. 2013, 18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Monahan, A.M.; Miller, I.S.; Nally, J.E. Leptospirosis: Risks during recreational activities. J. Appl. Microbiol. 2009, 107, 707–716. [Google Scholar] [CrossRef] [PubMed]
Animals 11 02722 g001 550
Figure 1. Geographical location of Leptospira interrogans antibody-negative and -positive samples of different wildlife species (A)—red fox; (B)—European badger, Eurasian lynx, golden jackal, stone marten, grey wolf; (C)—roe deer; (D)—red deer; (E)—chamois; (F)—nutria, brown hare, fallow deer, European mouflon) detected by MAT in Slovenia from 2019 to 2020.
Figure 1. Geographical location of Leptospira interrogans antibody-negative and -positive samples of different wildlife species (A)—red fox; (B)—European badger, Eurasian lynx, golden jackal, stone marten, grey wolf; (C)—roe deer; (D)—red deer; (E)—chamois; (F)—nutria, brown hare, fallow deer, European mouflon) detected by MAT in Slovenia from 2019 to 2020.
Animals 11 02722 g001
Table
Table 1. Samples from 249 free-ranging wild animals, harvested or found dead (*).
Table 1. Samples from 249 free-ranging wild animals, harvested or found dead (*).
Species Common NameLatin NameNo. of Animals
Alpine chamoisRupicapra rupicapra21
Brown hareLepus europaeus2
European badgerMeles meles2
European mouflonOvis musimon4
Fallow deerDama dama1
Golden jackalCanis aureus2
NutriaMyocastor coypus1
Red deerCervus elaphus22
Red foxVulpes vulpes97
Roe deerCapreolus capreolus80
Stone martenMartes foina12
* Eurasian lynxLynx lynx2
* Grey wolfCanis lupus3
Total samples249
Table
Table 2. Number of serum samples (Total no.) collected from different wildlife species in Slovenia from 2019 to 2020, testing positive (No. pos.) to antibody against Leptospira serovars.
Table 2. Number of serum samples (Total no.) collected from different wildlife species in Slovenia from 2019 to 2020, testing positive (No. pos.) to antibody against Leptospira serovars.
Common NameTotal No.No. Pos.Proportion of Positives (and CI)Serovars
Alpine chamois21210 (0–22)%IcteroBratTar------
Brown hare *20----------
European badger *20----------
European mouflon *40----------
Fallow deer *10----------
Red deer22732 (12–51)%IcteroBratPomGripSejroeAutCan--
Roe deer802025 (16–34)%IcteroBratPomGripSejroe----
European badger *20----------
Eurasian lynx22Large carnivores
86 (60–100)%		IcteroSejroe-------
Golden jackal22IcteroPomHardjoSejroeSax----
Gray wolf32IcteroGrip ------
Red fox973334 (25–43)%IcteroBratPomGripSejroeAusAutSaxCan
Stone
marten		12867 (40–93)%IcteroBratPomAusSax----
2497730.9 (25.2–36.7)%
* The proportion and CI of positive samples are not presented due to unreliability resulting from small sample size. Abbreviations: Icterohaemorrhagiae (Ictero), Bratislava (Brat), Pomona (Pom), Grippotyphosa (Grip), Australis (Aus), Autumnalis (Aut), Canicola (Can), Saxkoebing (Sax) and Tarassovi (Tar).
Table
Table 3. Serovars and antibody titres against Leptospira serovars in tested wild animals.
Table 3. Serovars and antibody titres against Leptospira serovars in tested wild animals.
Common NameSerovarsTitre
50100200400800≥1600
Alpine chamoisIctero010000
Tarassovi100000
Eurasian lynxIctero010000
Sejroe100000
Golden jackalIctero011000
Pomona000100
Hardjo010000
Sejroe000010
Saxkoebing000100
Gray wolfIctero110000
Grippo010000
Nutria Bratislava010000
Red foxIctero323012
Bratislava247210
Pomona213200
Grippo100000
Sejroe242100
Australis716021
Autumnalis001000
Canicola010100
Saxkoebing023100
Red deerIctero010000
Bratislava010000
Pomona110100
Grippo100000
Sejroe100000
Autumnalis010000
Canicola110000
Bratislava213000
Pomona001000
Australis211121
Saxkoebing100000
Roe deerIctero841000
Bratislava400000
Pomona100100
Grippo100000
Sejroe100000
Stone martenIctero020100
Table
Table 4. Outcome of chi-square homogeneity tests of differences in proportion of detected antibodies for L. interrogans between pairs of species. Numbers in the table are p-values; significant differences are in bold. The proportion of positive cases is given in parentheses.
Table 4. Outcome of chi-square homogeneity tests of differences in proportion of detected antibodies for L. interrogans between pairs of species. Numbers in the table are p-values; significant differences are in bold. The proportion of positive cases is given in parentheses.
Species (Proportion of Positive Cases)Mouflon (0%)Alpine Chamois (10%)Roe Deer (25%)Red Deer (32%)Red Fox (34%)Stone Marten (67%)Large Carnivores (86%)
Mouflon (0%)1.0000.5260.2550.1990.1580.0370.027
Alpine chamois (10%)0.5261.0000.1290.0800.0280.0020.001
Roe deer (25%)0.2550.1291.0000.5220.1940.0040.001
Red deer (32%)0.1990.0800.5221.0000.8440.0590.019
Red fox (34%)0.1580.0280.1940.8441.0000.0300.008
Stone marten (67%)0.0370.0020.0040.0590.0301.0000.376
Large carnivores (86%)0.0270.0010.0010.0190.0080.3761.000
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Žele-Vengušt, D.; Lindtner-Knific, R.; Mlakar-Hrženjak, N.; Jerina, K.; Vengušt, G. Exposure of Free-Ranging Wild Animals to Zoonotic Leptospira interrogans Sensu Stricto in Slovenia. Animals 2021, 11, 2722. https://doi.org/10.3390/ani11092722

AMA Style

Žele-Vengušt D, Lindtner-Knific R, Mlakar-Hrženjak N, Jerina K, Vengušt G. Exposure of Free-Ranging Wild Animals to Zoonotic Leptospira interrogans Sensu Stricto in Slovenia. Animals. 2021; 11(9):2722. https://doi.org/10.3390/ani11092722

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Žele-Vengušt, Diana, Renata Lindtner-Knific, Nina Mlakar-Hrženjak, Klemen Jerina, and Gorazd Vengušt. 2021. "Exposure of Free-Ranging Wild Animals to Zoonotic Leptospira interrogans Sensu Stricto in Slovenia" Animals 11, no. 9: 2722. https://doi.org/10.3390/ani11092722

APA Style

Žele-Vengušt, D., Lindtner-Knific, R., Mlakar-Hrženjak, N., Jerina, K., & Vengušt, G. (2021). Exposure of Free-Ranging Wild Animals to Zoonotic Leptospira interrogans Sensu Stricto in Slovenia. Animals, 11(9), 2722. https://doi.org/10.3390/ani11092722

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