Effect of an Immunomodulatory Feed Additive in Mitigating the Stress Responses in Lactating Dairy Cows to a High Concentrate Diet Challenge
, Ludovica M. E. Mammi 1, Alberto Palmonari 1,*, Ruben GarcÃa-González 2, James D. Chapman 2, Dereck J. McLean 2 and Andrea Formigoni 1
Robert J. Collier
Round 1
Reviewer 1 Report
General comments
1. Authors need to state what the objective of the study was in the introduction.
2. For all assays identified in the materials and methods please provide the inter and intra assay C.V.'s in order to assess the variability of the results.
Specific Comments
Page Line Comment
6 245 It is redundant to state "no significant differences detected. Just
state "no differences were detected"
Author Response
Dear Reviewer 1,
thank you for your revisions.
- We stated the objective of the study in the introduction.
- We reported the reference including the inter and intra assay C.V.'s.
- We fixed the redundance on line 245
Reviewer 2 Report
The manuscript was very well written.
The immunomodulatory effects of OmniGen-AF were small. There are no effects on inflammatory or oxidative status markers. It should be extensively indicated in the simple summary, abstract, and the discussion
When the challenge started, DIM was around 100. However, there was no comment about it. Cow in the first weeks of lactation is exposed to multiple stressors, including diet changes. OmmiGen-AF effects maybe were not observed due to the reduced stress conditions that cows were exposed to. This could be indicated.
Author Response
Dear Reviewer 2, thank you for your revision.
We changed as you suggested the simple summary, abstract, results and discussion.
