Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals, Housing, and Feeding
2.3. Experimental Design and Ginseng Preparation
2.4. Semen Samples Collection
2.5. Fresh Semen Evaluation
2.6. Dilution and Cryopreservation
2.7. Frozen-Thawed Semen Evaluation
2.7.1. Sperm Motility
2.7.2. Membrane Integrity
2.7.3. Lipid Peroxidation
2.7.4. Enzyme Activity
SOD
CAT
GPx
2.8. Fertility Test
2.9. Statistical Analysis
3. Results
3.1. Fresh Semen Quality
3.2. Frozen-Thawed Sperm Motility, Membrane Integrity, Lipid Peroxidation, and Enzyme Activities
3.3. Fertility Test
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ginseng Levels (mg/mL) | Parameters | ||
---|---|---|---|
MOT (%) | PMOT (%) | Membrane Integrity (%) | |
0 | 38.18 ± 0.56 BC | 24.88 ± 0.38 B | 26.14 ± 3.98 C |
0.25 | 59.47 ± 2.26 A | 30.82 ± 0.70 A | 48.30 ± 1.70 A |
0.50 | 46.22 ± 1.42 AB | 26.80 ± 2.10 AB | 35.71 ± 2.29 B |
0.75 | 38.47 ± 3.69 BC | 24.75 ± 0.90 B | 27.15 ± 0.88 BC |
1 | 30.67 ± 3.07 C | 25.25 ± 0.68 AB | 23.90 ± 1.48 C |
p-value | 0.0002 | 0.0274 | <0.0001 |
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Ratchamak, R.; Authaida, S.; Koedkanmark, T.; Boonkum, W.; Semaming, Y.; Chankitisakul, V. Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes. Animals 2023, 13, 2660. https://doi.org/10.3390/ani13162660
Ratchamak R, Authaida S, Koedkanmark T, Boonkum W, Semaming Y, Chankitisakul V. Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes. Animals. 2023; 13(16):2660. https://doi.org/10.3390/ani13162660
Chicago/Turabian StyleRatchamak, Ruthaiporn, Supakorn Authaida, Thirawat Koedkanmark, Wuttigrai Boonkum, Yoswaris Semaming, and Vibuntita Chankitisakul. 2023. "Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes" Animals 13, no. 16: 2660. https://doi.org/10.3390/ani13162660
APA StyleRatchamak, R., Authaida, S., Koedkanmark, T., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2023). Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes. Animals, 13(16), 2660. https://doi.org/10.3390/ani13162660