Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Assay Comparison Data
2.2. Estimation of Conversion Formulae
2.3. Integration into a Web App
3. Results
3.1. Available Assays and Data Sources
3.2. Assay Pairs
3.3. Web App Usage
3.4. Example: Comparison of Published Cut-Offs
4. Discussion
4.1. Use and Limitations of the App
4.2. Comparison of Cut-Off Values
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Assay Name | Assay Family | |
---|---|---|
A | ADVIA Centaur Insulin Assay, Siemens Healthcare | CLIA |
B | Coat-A-Count Insulin, Siemens Healthcare | RIA |
C | Immulite 1000 Insulin Assay, Siemens Healthcare | CLIA |
D | Immulite 2000 Insulin Assay, Siemens Healthcare | CLIA |
E | Immulite 2000XPi Insulin Assay, Siemens Healthcare | CLIA |
F | Insulin(e) IRMA KIT, Immunotech, Beckman Coulter | RIA |
G | Equine Insulin ELISA, Mercodia | ELISA |
H | Porcine Insulin RIA, Merck Millipore | RIA |
Source | Assays Compared | Number of Samples | Comments |
---|---|---|---|
Öberg et al., 2011 [15] | B vs. G | 80 | |
Tinworth et al., 2011 [16] | B vs. G | 18 | |
Borer-Weir et al., 2012 [17] | B vs. G | 29 | |
Warnken et al., 2016 [19] | A vs. G A vs. H G vs. H | 36 39 36 | |
Carslake et al., 2017 [20] | B vs. D | 16 | |
Carslake et al., 2021 [26] | D vs. E | 39 | |
De Laat et al., 2022 [34] | E vs. G | 302 | Raw data kindly provided by Boehringer Ingelheim |
Durham, unpublished | C vs. E | 70 | Diagnostic samples |
Fey, unpublished | A vs. B | 496 | Diagnostic samples |
Warnken and Delarocque, unpublished | C vs. G A vs. G A vs. C | 60 77 60 | Ethics committee file number 33.8-42502-04-17/2646 |
Warnken, unpublished | C vs. G C vs. F F vs. G | 21 21 131 | Ethics committee file number 3.14-42502-04-13/1259 |
Warnken, unpublished | A vs. G | 30 | Diagnostic samples |
Reference | Assay | Feeding | Outcome | CO Metric | CO (µIU/mL) | Conv. CO (µIU/mL) |
---|---|---|---|---|---|---|
Lindase et al., 2021 [35] | Mercodia Equine | Fasted | EHC | Youden’s index | 9.5 | 9.5 |
de Laat et al., 2022 [34] | Mercodia Equine | Fasted | Cluster | Balanced sens. and spec. 1 | 10.4 | 10.4 |
Olley et al., 2019 [36] | Immulite 1000 or 2000 | Fasted | CGIT | Youden’s index | 5.2 | 22 |
Menzies-Gow et al., 2017 [37] | Coat-a-Count | Unfasted | Laminitis | Youden’s index | 21.8 | 26 |
Meier et al., 2018 [38] | ADVIA Centaur | Fasted | High NSC diet laminitis | CART | 8.5 | 28 |
Carter et al., 2009 [39] | Coat-a-Count | Unfasted (hay) | Pasture associated laminitis | AUC in ROC | 32 | 33 |
EEG 2020 [40] | Immulite 2000XPi | Unfasted (hay) | 31 | 41 | ||
EEG 2020 [40] | Immulite 1000 | Unfasted (hay) | 20 | 49 | ||
Köller et al., 2016 [41] | Immulite 2000 | Unfasted (hay) | Healthy | 95% confidence interval 2 | 21 | 49 |
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Delarocque, J.; Feige, K.; Carslake, H.B.; Durham, A.E.; Fey, K.; Warnken, T. Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses. Animals 2023, 13, 2704. https://doi.org/10.3390/ani13172704
Delarocque J, Feige K, Carslake HB, Durham AE, Fey K, Warnken T. Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses. Animals. 2023; 13(17):2704. https://doi.org/10.3390/ani13172704
Chicago/Turabian StyleDelarocque, Julien, Karsten Feige, Harry B. Carslake, Andy E. Durham, Kerstin Fey, and Tobias Warnken. 2023. "Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses" Animals 13, no. 17: 2704. https://doi.org/10.3390/ani13172704
APA StyleDelarocque, J., Feige, K., Carslake, H. B., Durham, A. E., Fey, K., & Warnken, T. (2023). Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses. Animals, 13(17), 2704. https://doi.org/10.3390/ani13172704