Next Article in Journal
Heat Stress Mitigation: Impact of Increased Cooling Sessions on Milk Yield and Welfare of Dairy Buffaloes in a Semiarid Summer
Previous Article in Journal
In Vitro Evaluation of the Adsorption Efficacy of Biochar Materials on Aflatoxin B1, Ochratoxin A, and Zearalenone
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Animals
Volume 13
Issue 21
10.3390/ani13213313
animals-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents

by
Min Hui
1,2,3,†,
Yu Zhang
4,†,
Aiyang Wang
1,3 and
Zhongli Sha
1,2,3,*
1
Department of Marine Organism Taxonomy & Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2
Laoshan Laboratory, Qingdao 266237, China
3
Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4
College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Animals 2023, 13(21), 3313; https://doi.org/10.3390/ani13213313
Submission received: 31 August 2023 / Revised: 18 October 2023 / Accepted: 23 October 2023 / Published: 25 October 2023
(This article belongs to the Section Animal Genetics and Genomics)
Browse Figures
Versions Notes

Abstract

:

Simple Summary

Due to limited reporting of genomic resources for deep-sea organisms, studies of their adaptive mechanisms for extreme environments have been hindered. A genome survey of the snail Provanna glabra, a representative species in deep-sea chemosynthetic ecosystem, was conducted based on Illumina sequencing. Genome size, repetitive elements, heterozygosity, and other genome features were estimated and characterized. A draft genome and a complete mitochondrial genome were assembled for P. glabra, and candidate molecular markers were identified. Phylogenetic analysis based on mitochondrial genes was performed. The results provide effective genome information for P. glabra and supply a basis for further high-quality genome map construction and biogeography study of this deep-sea chemosynthetic snail.

Abstract

The snail P. glabra is an endemic species in deep-sea chemosynthetic ecosystems of the Northwest Pacific Ocean. To obtain more genetic information on this species and provide the basis for subsequent whole-genome map construction, a genome survey was performed on this snail from the hydrothermal vent of Okinawa Trough. The genomic size of P. glabra was estimated to be 1.44 Gb, with a heterozygosity of 1.91% and a repeated sequence content of 69.80%. Based on the sequencing data, a draft genome of 1.32 Gb was assembled. Transposal elements (TEs) accounted for 40.17% of the entire genome, with DNA transposons taking the highest proportion. It was found that most TEs were inserted in the genome recently. In the simple sequence repeats, the dinucleotide motif was the most enriched microsatellite type, accounting for 53% of microsatellites. A complete mitochondrial genome of P. glabra with a total length of 16,268 bp was assembled from the sequencing data. After comparison with the published mitochondrial genome of Provanna sp. from a methane seep, 331 potential single nucleotide polymorphism (SNP) sites were identified in protein-coding genes (PCGs). Except for the cox1 gene, nad2, nad4, nad5, and cob genes are expected to be candidate markers for population genetic and phylogenetic studies of P. glabra and other deep-sea snails. Compared with shallow-water species, three mitochondrial genes of deep-sea gastropods exhibited a higher evolutionary rate, indicating strong selection operating on mitochondria of deep-sea species. This study provides insights into the genome characteristics of P. glabra and supplies genomic resources for further studies on the adaptive evolution of the snail in extreme deep-sea chemosynthetic environments.

1. Introduction

Deep-sea hydrothermal vent is one of the chemosynthetic ecosystems primarily relying on chemoautotrophs to sustain their productivity and is characterized by high hydrostatic pressure, low oxygen, and being rich in hazardous substances, such as heavy metals, methane, and hydrogen sulfide [1,2]. Vents are usually found in active mid-ocean ridges and back-arc diffusion centers, and during the eruption, the fluid’s temperature can exceed 400 °C [3,4]. Despite the harsh environment, many macrobenthos are found densely distributed around hydrothermal vents, e.g., fish, polychaetes, crustaceans, and mollusks. However, how these organisms adapt to the extreme environment remains unclarified.
Genome information could provide insights into the evolution and adaptive mechanisms of lives inhabiting deep-sea chemosynthetic ecosystems [5,6,7]. Benefiting from the development of next-generation sequencing technologies, whole-genome maps of several animal species from hydrothermal vents or cold seeps have been completed, and the genetic mechanisms of their adaptation to the extreme environments have been explored in bivalve, gastropod, tubeworm, and sea cucumber [8,9,10,11]. However, genome resources for deep-sea vent and seep species are still scarce in comparison with shallow-water species. Before whole-genome sequencing and assembly, it is necessary to study the genome size and characteristics of the target species in order to decide the sequencing strategy and depth.
Gastropod is a dominant group in deep-sea chemosynthetic ecosystems [12]. The Provannidae family of gastropods is large in number and widely distributed in hydrothermal vents, cold seeps, sunken wood, and vertebrate falls [12,13,14]. Provanna glabra Okutani, Tsuchida & Fujikura, 1992 belonging to Provannidae is one of the common species endemic to the deep-sea hydrothermal vent and cold seep areas in the Northwest Pacific. Thus far, only the mitochondrial genome of Provanna sp. is assembled, and phylogenetic analyses are conducted for Provanna snails and other related species [15]. Therefore, more genetic and genomic resources for this typical gastropod in chemosynthetic ecosystems are very important and imperative to facilitate the study of its adaptive evolution to extreme environments.
Genome survey sequencing (GSS) is an efficient approach for generating genome information, which can serve as a reference for population genomic and evolution studies and supply background for the subsequent whole-genome map construction. In this study, GSS for P. glabra from a hydrothermal vent in the Okinawa Trough was performed, and sequences were assembled into a draft genome. Genome size, heterozygosity, and repeat content were estimated, and candidate molecular markers were identified. A mitochondrial genome was also assembled, and phylogenetic analysis for gastropods was conducted. Candidate molecular markers were also identified. The results are expected to add more genomic resources and provide a basic understanding of the P. glabra genome.

2. Materials and Methods

2.1. Sample Collection and DNA Extraction

In this study, only one snail from the deep sea was used for the experiment, and all efforts were made to minimize the suffering of the snail. The experiments were conducted in strict accordance with the guidelines of the Regulations on the Management of Animal Experiments (20170301, https://www.gov.cn/gongbao/content/2017/content_5219148.htm, accessed on 10 July 2018) constituted by the Chinese government, and the Regulations on the Management of Animal Experiment Safety (2020-37) constituted by the Institute of Oceanology, Chinese Academy of Sciences.
In July 2018, samples of P. glabras were collected from the Iheya North hydrothermal vent in Okinawa Trough (126°53.80′ E, 27°47.21′ N, depth 961.24 m) (Figure 1a) during the cruise by the scientific research vessel (RV) KEXUE (Institute of Oceanology, Chinese Academy of Sciences, China). Samples were taken by the remotely operated vehicle (ROV) Quasar MkII on RV KEXUE, frozen in liquid nitrogen, and stored at −80 °C for subsequent DNA extraction.

2.2. DNA Extraction and Sequencing

DNA of the muscle tissue of the individual was extracted with the traditional phenol-chloroform method [16], and its quality and concentration were assessed by 1.0% agarose and a NanoDrop 2000 spectrophotometer. The tested DNA was used for sequencing library construction using the Illumina library preparation kit (Illumina, San Diego, CA, USA) according to the instructions. The paired-end libraries with an insert length of 350 bp were then sequenced on the Illumina Hiseq X-ten sequencing system (Illumina, San Diego, CA, USA).

2.3. Quality Control, K-mer Analysis, and Assembly of Sequences

The raw reads were then filtered using Trimmomatic v.0.39 [17] to produce clean reads for further analysis. Data filtering was strictly performed according to the following criteria: (i) trimming reads with adaptors; (ii) deleting low-quality reads with unidentified nucleotides (N) content more than 8%; (iii) removing reads with a high percentage (>20%) of low-quality bases (quality score less than 10). K-mer (K = 17) analysis was performed by Jellyfish to calculate genome size, repetitive sequence content, and heterozygosity [18]. The draft genome was de novo assembled based on high-quality reads with SOAPdenovo 2 [19] by setting K-mer = 41. Completeness of the assembled genome was estimated against the metazoa_odb10 database using BUSCO (Benchmarking Universal Single-Copy Orthologs) v.5.5.0 [20].

2.4. Annotation of Repetitive Sequences and Identification of Microsatellites

Repeat sequence identification was performed on the assembled genome. Tandem repeats in the genome were identified through TRF (Tandem Repeats Finder) 4.0 (http://tandem.bu.edu/trf/trf.html, accessed on 26 December 2022). For interspersed repeats (TEs) in the genome, a repeat sequence database of the genome was set up by de novo prediction of RepeatModeler (http://www.repeatmasker.org/RepeatModeler/, accessed on 28 December 2022) and LCR-FINDER [21], and then RepeatMasker was further used to annotate and classify the repeat sequences. Meanwhile, RepeatMasker (http://www.repeatmasker.org/, accessed on 28 December 2022) and RepeatProteinMask were selected to identify TEs by comparing genome sequences with the reported repeat sequences in the RepBase database [22]. The divergence rates of different types of TEs were calculated by using RepeatMasker based on the merged repeat sequence database. Simple sequence repeats (SSRs) in the genome were detected using the MIcroSAtellite identification system (MISA) v. 2.1 [23].

2.5. Mitochondrial Genome Assembly and SNP Screening

With clean reads, the mitochondrial genome of P. glabra was assembled by NOVOPlasty v. 4.2.1 [24]. During assembly, the mitochondrial cytochrome c oxidase subunit I (cox1) sequence of P. glabra was used as a seed file and K-mer was set as 33. The newly assembled mitochondrial genome was annotated on the MITOS2 website (http://mitos2.bioinf.uni-leipzig.de/, accessed on 18 April 2023) and Geseq (https://chlorobox.mpimp-golm.mpg.de/geseq.html, accessed on 18 April 2023) followed by manual correction. The reassembled mitochondrial genome sequence was compared with the previously published mitochondrial genome sequence of Provanna sp. [15] using BioEdit version 7.2 in order to identify candidate SNPs. Nucleotide sequences of PCGs were translated into amino acid sequences on the NovoPro website (https://www.novopro.cn/tools/translate.html, accessed on 20 April 2023). The non-synonymous loci were then screened by comparing the amino acid sequences of the two mitochondrial genomes with BioEdit version 7.2.

2.6. Phylogenetic and Selective Pressure Analyses of Mitochondrial Genes

From the NCBI database, we downloaded complete mitochondrial genome sequences of another 20 gastropod species, including four other species from a deep-sea vent or seep environments and one cephalopod species (Amphioctopus marginatus, outgroup) (Table S1). Based on the TBtools-II bioinformatics platform [25], 13 PCG sequences of 22 species were compared using MUSCLE, and a phylogenetic tree was constructed using IQ-tree under an mtInv+F+R4 model of nucleotide sequences with maximum likelihood (ML) method. By setting the clade of deep-sea chemosynthetic species as foreground lineage (Abyssochrysodea) and shallow-water species, Bolinus brandaris, Conus borgesi, and Monoplex parthenopeus as background lineage, EasyCodeML was used to evaluate the selection pressure in the mitochondrial genes from different lineage [26]. With the branch model, the ω (dN/dS) value of the one-ratio Model 0, the ω0 of the background lineage, and the ω1 of the foreground lineage of the two-ratio Model 2 were evaluated, respectively. The two models were compared according to the likelihood ratio test (LRT, p-value < 0.05) to determine whether the evolutionary rate (dN/dS) of deep-sea species was different from that of shallow-water species.

3. Results and Discussion

3.1. Genome Sequencing, Size Estimation and Assembly

Genomic sequencing of P. glabra generated a total of 63.54 Gb raw reads. After filtering, a total of 55.70 Gb of clean data was obtained with Q30 at 92.98% and GC content at 44.48%, indicating high sequencing quality (Table 1). The clean data were deposited in the NCBI SRA database with the accession number PRJNA990319. By K-mer analysis, the estimated genome size was 1.45 Gb. After correction, genome size was revised into 1.44 Gb with heterozygous rate and repetitive sequence rate of 1.91% and 69.80%, respectively (Figure 1b), indicating a complex genome. Compared with other snails from deep-sea chemosynthetic environments, such as Ifremeria nautilei (0.88 Gb), Alviniconcha hessleri (0.92 Gb), Chrysomallon squamiferum (~0.46 Gb), and Gigantopelta aegis (~1.29 Gb), P. glabra exhibited relative larger genome sizes, as well as high heterozygosity and repeat content [27,28]. Considering the function of repetitive elements in genome evolution [29], the accumulation of repetitive sequences might contribute to the genome size variation among P. glabra and other deep-sea snails.
Genome assembly resulted in 4,132,680 contigs with an N50 length of 437 bp and 3,534,913 scaffolds with an N50 length of 581 bp (Table 1). The total length of the scaffolds was 1.32 Gb, covering about 92% of the genome, and the GC content of the assembly was 45.52%. GC statistics on the assembly showed that the GC content of sliding windows was populated without other independent sequence clusters, indicating that the sequencing sample was not contaminated by alien species (Figure 1c). BUSCO analysis showed that 67.19% of orthologs were missing in the assembled draft genome of P. glabra (Table S2). Therefore, it shows that the quality of the genome assembly is low only based on Illumina short-read sequencing data. Long-read sequencing technologies, such as PacBio SMRT (Single Molecule Real Time) and ONT (Oxford Nanopore Technology, Oxford, UK), are recommended in the subsequent high-quality genome map construction of P. glabra. Moreover, Hi-C (High-throughput chromosome conformation capture) technology can be used to link contigs to different chromosomes. In this study, the genome survey results have provided preliminary insights into genome characteristics of P. glabra, laying the foundation for further whole-genome sequencing and assembly and significantly enriching genomic resources of deep-sea life.

3.2. Repeated Elements in the Genome of P. glabra

Repeat elements, especially transposon, are an important part of the eukaryotic genome and a source of genetic variation [30,31,32]. In total, 636 Mb of repeated elements were screened out, representing 48.03% of the entire assembled draft genome of P. glabra (Table S2), in which, 532 Mb of TEs were included. DNA transposon (9.39%) was the most dominant type among all TEs. It was followed by long interspersed elements (LINEs, 6.17%), short interspersed elements (SINEs, 4.53%), and long terminal repeats (LTRs, 3.75%) (Table 2). Comparing the TE content of P. glabra with that in seven other gastropod species with genome assembly (three from deep sea and four from the shallow sea (Table S4) [28,33,34,35], it has been found that DNA transposons are the most dominant type in deep-sea gastropods, while in most shallow-water species, LINEs are abundant. It indicates that DNA transposons might play important roles in the genome evolution of deep-sea gastropods.
Observing the divergence rate of TEs can provide us with a deeper understanding of the evolutionary history of TEs. A higher divergence rate represents a greater number of mutations accumulated, which indicates that TEs have existed in the genome for a longer period, while conversely, it suggests a shorter insertion time. Most TEs in the P. glabra genome were found with peaks of the divergence rate lower than 10% except that the peak of divergence rate in DNA transposon was lower than 20% (Figure 1d). It indicates that most TEs in the genome of P. glabra burst recently and are relatively young [36]. Moreover, it has been reported that TEs can produce a variety of mutations leading to high genomic plasticity and are sensitive to environmental changes [37,38,39]. For example, a substantial proportion of TE insertions and their frequency in Drosophila are correlated with environmental variables, such as temperature and rainfall [40]. Besides functioning in local adaptation, TEs in Drosophila have also been involved in resistance to microbial infection and toxic substances by inserting themselves into protein-coding sequences of key genes [41]. More importantly, in another study of population genetics of P. glabra from different chemosynthetic environments, some TEs were discovered to be under selection and involved in the adaptation to the local environment [42]. Hence, these findings together with our results suggest that TEs within P. glabra might be rapidly activated during its invasion of deep-sea chemosynthetic environments, and the proliferation of young TEs in P. glabra might function in the adaptive evolution of this snail.

3.3. Microsatellites in the Genome of P. glabra

Microsatellites, also known as SSRs, are widely distributed in the genomes of both eukaryotes and prokaryotes, usually in the form of low repeats of 1–6 bp. A total of 2,387,170 microsatellite loci were found in the P. glabra genome assembly. Statistics of microsatellite loci showed that the majority of them were dinucleotide motifs (53%), followed by trinucleotide (22.04%), tetranucleotide (10.89%), pentanucleotide (1.77%), and hexanucleotide motifs (0.29%) (Figure 2, Table S5). The most prevalent patterns in each motif type were AC/GT (64.06%) in dinucleotides, AAT/ATT (41.39%) in trinucleotides, AAAC/GTTT (23.63%) in tetranucleotides, AATAC/ATTGT (20.78%) in pentanucleotides, and ACAGAG/CTCTGT (17.47%) in hexanucleotides. With characteristics of high polymorphism and codominant, microsatellites are excellent markers in genetic diversity and genetic structure studies [43], and polymorphism is usually higher in dinucleotide and trinucleotide motifs than in other microsatellite types. Therefore, in the future study of the biogeography of P. glabra, dinucleotide, and trinucleotide SSRs should be prioritized as molecular markers [44]. Our study supplies the basis for marker development of the deep-sea snail. Moreover, once the high-quality genome map of P. glabra is completed, population re-sequencing based on the genome is expected to provide more insights into the genetic evolution of the species.

3.4. Phylogeny Based on Mitochondrial Genomes and SNP Candidates

Mitochondrial genes are frequently employed in phylogenetic and population genetic analyses. A complete mitochondrial genome of P. glabra with a length of 16,268 bp was assembled from the genome survey data and deposited in GenBank under the accession number OR209184 (Figure 3). After annotation, we identified 13 PCGs, 2 rRNA genes, and 22 tRNA genes (Table S6).
The obtained mitochondrial genome sequence shows high similarity to that of Provanna sp. Published by Xu et al., 2015 [15], in which the snail was collected from a methane seep on the continental slope of the South China Sea. A longer control region (934 bp VS. 856 bp) was obtained in our study. By sequence alignment of 13 PCGs in the two mitochondrial genomes, a total of 331 potential SNPs were identified (Table 3). Among the PCGs, the cox3 gene exhibited the highest mutation rate (3.72%), followed by cob (3.68%), while the atp6 and atp8 genes had the lowest proportion of mutations. It was also noted that most nad genes showed a high proportion of mutations (2.02~3.67%) (Table 3). Cox1 is usually used as a molecular marker to distinguish inside and interspecific genetic evolutionary information [45,46]. In this study, cox1 with a length of 1536 showed a mutation rate of 2.15%. Considering the high mutation rate (>3%) and sequence length (>1000 bp, proper for primer design) of genes, nad2, nad4, nad5, and cob are suggested to be candidate markers utilized for phylogenetic and population genetic analysis of P. glabra and other deep-sea snails.
Mitochondria in eukaryotes can provide energy for cell life by driving oxidative phosphorylation through the respiratory chain and proton pump, and it has been demonstrated that mitochondrial genes would be under selection in extreme environments [47]. Therefore, we performed phylogenetic analysis and examined selection pressure on mitochondrial genes in deep-sea gastropods, including P. glabra.
Based on the 13 PCGs, a phylogenetic tree of gastropoda was constructed (Figure 4a). It was found that P. glabra and four other species from deep-sea vents or seeps clustered together and all belonged to Abyssochrysoidea. Provanna glabra showed the closest relationship with Provanna sp. By further comparison of mitochondrial PCGs of Provanna sp and P. glabra from different seep and vent environments, 20 non-synonymous sites were identified, most of which were located in nad genes (Table 3). Specifically, six and five non-synonymous sites were detected in nad2 and nad5, followed by nad3 with three non-synonymous sites. One non-synonymous site was found in nad4, nad4l, and nad6, respectively. Meanwhile, the non-synonymous mutation was also detected in the cox3 (two sites) and cob (one site) gene. It indicates that the mutational events occurring in these mitochondrial genes may impact the adaptive evolution process of Provanna snails from different vent and seep environments [48,49,50,51,52]. In other words, the isolation and environmental differences of vent and seep might promote the genetic divergence of the two related species as suggested in the study for vent and seep squat lobsters and mussels [53,54].
Moreover, a positive selection of mitochondrial genes has been also observed in various deep-sea benthic animals, such as mussels, shrimps and crabs [46,55,56]. In our study, the selection pressure suffered in different lineages was also estimated. Based on the phylogenetic tree, ω (dN/dS) values of deep-sea Abyssochrysoidea lineage (foreground branch) and shallow-water lineage (Tonnoidea, Conoidea, and Muricoidea, foreground branch) were calculated and compared. The results based on the branch model showed that in both M0 and M2 models, ω (dN/dS) values for all PCGs were lower than 1, signifying strong purifying selection operating on the mitochondrial genomes within the two gastropod lineages (Table S7). When compared, ω values of four genes were significantly different (p < 0.01) between the two lineages, suggesting different evolutionary rates (Figure 4b, Table S7). Among them, cox2, cox3, and nad4 exhibited higher ω values in deep-sea species compared with the shallow-water species, with notable distinctions observed in the nad4 gene. It is known that NADH dehydrogenase is the largest and most crucial proton pump in the respiratory chain [49,52]. The significant evolutionary rate differences in the nad genes between deep-sea and shallow-water gastropods suggest that such genes may experience greater selective pressure in the adaptive evolution of deep-sea gastropods. Additionally, cytochrome oxidase has been demonstrated to play an important role in anaerobic adaptation, with its catalytic core encoded by three PCGs (cox1, cox2, cox3) [57]. Therefore, the rapid evolution of cox2, and cox3 in deep-sea gastropods might aid them in better adapting to deep-sea anaerobic environments and other stressors. In summary, our results reveal that the mitochondrial cox and nad genes in deep-sea gastropods not only are under selection in different vent and seep environments but have also evolved at a distinct rate when compared with shallow-water species, indicating their important roles in the adaptive evolution of deep-sea gastropods.

4. Conclusions

In this study, a genome survey of P. glabra from a hydrothermal vent in Okinawa Trough revealed a complex snail genome, with a relatively large genome size, high heterozygosity, and repeat sequence. Sequence assembling resulted in a draft genome of P. glabra. In the genome assembly, TEs were found inserted in the genome recently and they accounted for high proportions of the genome, which might contribute to the genome evolution of the snail. Large numbers of SSRs were identified, supplying the basis for the development of molecular markers. Moreover, a complete mitochondrial genome was assembled from the sequencing data. Potential SNPs were screened out by comparing mitochondrial gene sequences obtained in this study with the published mitochondrial genome of Provanna sp. from a methane seep in the South China Sea. In addition to cox1, it was proposed that nad2, nad4, nad5, and cob can be employed as markers for population genetics and phylogenetic analysis of P. glabra and its relatives. Non-synonymous mutations predominantly occur in nad genes, which may play an important role in Provanna snails’ adaptation to different environments. The phylogenetic tree based on mitochondrial genomes established the evolutionary position of P. glabra, and rapid evolutionary rates were detected in three mitochondrial genes of deep-sea species, suggesting that mitochondrion experienced stronger selection compared with shallow-water gastropods. These results provide genome resources, evidence, and candidate molecular markers for the studies of adaptive evolution in snails from deep-sea chemosynthetic ecosystems and lay a foundation for the subsequent high-quality genome map construction of P. glabra.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ani13213313/s1, Table S1: Information on species included in the phylogenetic analysis; Table S2: BUSCO evaluation of the draft genome of P. glabra; Table S3: Statistics of repeat sequences in the draft genome of P. glabra; Table S4: Proportions of TE types in different species; Table S5: Statistics of SSRs in the draft genome of P. glabra; Table S6: Information on mitochondrial genome of P. glabra; Table S7: Evolutionary rate comparison of mitochondrial genes in the deep-sea and shallow-water gastropod lineages.

Author Contributions

Z.S. and M.H. conceived the study. M.H. and Z.S. collected samples. M.H. carried out the lab work. M.H., Y.Z. and A.W. analyzed the data and interpreted the findings. M.H. and Y.Z. authored drafts in the article. M.H., A.W. and Z.S. revised the article and approved the final draft. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA22050302 and XDB42000000), the National Science Foundation for Distinguished Young Scholars (42025603), and the Science and Technology Innovation Project of Laoshan Laboratory (LSKJ202203104).

Institutional Review Board Statement

The animal study protocol was approved by the Institutional Review Board of Institute of Oceanology, Chinese Academy of Sciences (protocol code 2018-0502 and date of approval 9 May 2018).

Informed Consent Statement

Not applicable.

Data Availability Statement

The data supporting the conclusions of this article will be made available by the authors without undue reservation. The clean data used in the genome survey are already publicly available in the NCBI SRA database with the accession number PRJNA990319. The mitochondrial genome sequence of the snail is deposited in GenBank under the accession number OR209184.

Acknowledgments

The samples were collected by RV KEXUE. We wish to thank the crews for their help during the collection of samples.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Levin, L.A.; Baco, A.R.; Bowden, D.A.; Colaco, A.; Cordes, E.E.; Cunha, M.R.; Demopoulos, A.W.J.; Gobin, J.; Grupe, B.M.; Le, J.; et al. Hydrothermal vents and methane seeps: Rethinking the sphere of influence. Front. Mar. Sci. 2016, 3, 72. [Google Scholar] [CrossRef]
  2. Brown, W.M.; George, M., Jr.; Wilson, A.C. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. USA 1979, 76, 1967–1971. [Google Scholar] [CrossRef] [PubMed]
  3. Georgieva, M.N.; Little, C.T.S.; Maslennikov, V.V.; Glover, A.G.; Ayupova, N.R.; Herrington, R.J. The history of life at hydrothermal vents. Earth-Sci. Rev. 2021, 217, 103602. [Google Scholar] [CrossRef]
  4. German, C.R.; Livermore, R.A.; Baker, E.T.; Bruguier, N.I.; Connelly, D.P.; Cunningham, A.P.; Morris, P.; Rouse, I.P.; Statham, P.J.; Tyler, P.A. Hydrothermal plumes above the East Scotia Ridge: An isolated high-latitude back-arc spreading centre. Earth Planet. Sci. Lett. 2000, 184, 241–250. [Google Scholar] [CrossRef]
  5. German, C.R.; Ramirez-Llodra, E.; Baker, M.C.; Tyler, P.A.; ChEss Scientific Steering Committee. Deep-water chemosynthetic ecosystem research during the census of marine life decade and beyond: A proposed deep-ocean road map. PLoS ONE 2011, 6, e23259. [Google Scholar] [CrossRef] [PubMed]
  6. Dubilier, N.; Bergin, C.; Lott, C. Symbiotic diversity in marine animals: The art of harnessing chemosynthesis. Nat. Rev. Microbiol. 2008, 6, 725–740. [Google Scholar] [CrossRef]
  7. Sogin, E.M.; Leisch, N.; Dubilier, N. Chemosynthetic symbioses. Curr. Biol. 2020, 30, R1137–R1142. [Google Scholar] [CrossRef]
  8. Sun, J.; Chen, C.; Miyamoto, N.; Li, R.; Sigwart, J.D.; Xu, T.; Sun, Y.; Wong, W.C.; Ip, J.C.H.; Zhang, W.; et al. The scaly-foot snail genome and implications for the origins of biomineralised armour. Nat. Commun. 2020, 11, 1657. [Google Scholar] [CrossRef]
  9. Ip, J.C.; Xu, T.; Sun, J.; Li, R.; Chen, C.; Lan, Y.; Han, Z.; Zhang, H.; Wei, J.; Wang, H.; et al. Host-Endosymbiont genome integration in a deep-sea chemosymbiotic clam. Mol. Biol. Evol. 2021, 38, 502–518. [Google Scholar] [CrossRef]
  10. Zhang, L.; He, J.; Tan, P.; Gong, Z.; Qian, S.; Miao, Y.; Zhang, H.Y.; Tu, G.; Chen, Q.; Zhong, Q.; et al. The genome of an apodid holothuroid (Chiridota heheva) provides insights into its adaptation to a deep-sea reducing environment. Commun. Biol. 2022, 5, 224. [Google Scholar] [CrossRef]
  11. Wang, M.; Ruan, L.; Liu, M.; Liu, Z.; He, J.; Zhang, L.; Wang, Y.; Shi, H.; Chen, M.; Yang, F.; et al. The genome of a vestimentiferan tubeworm (Ridgeia piscesae) provides insights into its adaptation to a deep-sea environment. BMC Genom. 2023, 24, 72. [Google Scholar] [CrossRef]
  12. Sasaki, T.; Warén, A.; Kano, Y.; Okutani, T.; Fujikura, K. Gastropods from recent hot vents and cold seeps: Systematics, diversity and life strategies. In The Vent and Seep Biota: Aspects from Microbes to Ecosystems; Steffen, K., Ed.; Springer: Dordrecht, The Netherlands, 2010; pp. 169–254. [Google Scholar]
  13. Bouchet, P.; Rocroi, J.P.; Hausdorf, B.; Kaim, A.; Kano, Y.; Nützel, A.; Parkhaev, P.; Schrödl, M.; Strong, E.E. Revised classification, nomenclator and typification of Gastropod and Monoplacophoran families. Malacologia 2017, 61, 1–526. [Google Scholar] [CrossRef]
  14. Colgan, D.J.; Ponder, W.F.; Beacham, E.; Macaranas, J. Molecular phylogenetics of Caenogastropoda (Gastropoda: Mollusca). Mol. Phylogenet. Evol. 2007, 42, 717–737. [Google Scholar] [CrossRef] [PubMed]
  15. Xu, T.; Sun, J.; Chen, C.; Qian, P.Y.; Qiu, J.W. The mitochondrial genome of the deep-sea snail Provanna sp. (Gastropoda: Provannidae). Mitochondrial DNA A 2016, 27, 4026–4027. [Google Scholar] [CrossRef] [PubMed]
  16. Sambrook, J.; Russell, D.W. (Eds.) Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Long Island, NY, USA, 2001. [Google Scholar]
  17. Bolger, A.M.; Lohse, M.; Usadel, B. Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 2014, 30, 2114–2120. [Google Scholar] [CrossRef]
  18. Liu, B.; Shi, Y.; Yuan, J.; Hu, X.; Zhang, H.; Li, N.; Li, Z.; Chen, Y.; Mu, D.; Fan, W. Estimation of genomic characteristics by analyzing k-mer frequency in de novo genome projects. Quant. Biol. 2013, 35, 62–67. [Google Scholar] [CrossRef]
  19. Luo, R.B.; Liu, B.H.; Xie, Y.L.; Li, Z.Y.; Huang, W.H.; Yuan, J.Y.; He, G.Z.; Chen, Y.X.; Pan, Q.; Liu, Y.J.; et al. SOAPdenovo2: An empirically improved memory-efficient short-read de novo assembler. Gigascience 2012, 1, 18. [Google Scholar] [CrossRef]
  20. Simao, F.A.; Waterhouse, R.M.; Ioannidis, P.; Kriventseva, E.V.; Zdobnov, E.M. BUSCO: Assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics 2015, 31, 3210–3212. [Google Scholar] [CrossRef]
  21. Xu, Z.; Wang, H. LTR_FINDER: An efficient tool for the prediction of full-length LTR retrotransposons. Nucleic Acids Res. 2007, 35, W265–W268. [Google Scholar] [CrossRef]
  22. Bao, W.; Kojima, K.K.; Kohany, O. Repbase Update, a database of repetitive elements in eukaryotic genomes. Mob. DNA 2015, 6, 11. [Google Scholar] [CrossRef]
  23. Thiel, T.; Michalek, W.; Varshney, R.; Graner, A. Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor. Appl. Genet. 2003, 106, 411–422. [Google Scholar] [CrossRef] [PubMed]
  24. Dierckxsens, N.; Mardulyn, P.; Smits, G. NOVOPlasty: De novo assembly of organelle genomes from whole genome data. Nucleic Acids Res. 2017, 45, e18. [Google Scholar] [CrossRef] [PubMed]
  25. Chen, C.; Wu, Y.; Li, J.; Wang, X.; Zeng, Z.; Xu, J.; Liu, Y.; Feng, J.; Chen, H.; He, Y.; et al. TBtools-II: A "One for All, All for One" bioinformatics platform for biological big-data mining. Mol. Plant 2023, in press. [Google Scholar] [CrossRef] [PubMed]
  26. Gao, F.L.; Chen, C.J.; Arab, D.A.; Du, Z.G.; He, Y.H.; Ho, S.Y.W. EasyCodeML: A visual tool for analysis of selection using CodeML. Ecol. Evol. 2019, 9, 3891–3898. [Google Scholar] [CrossRef]
  27. Bonnivard, E.; Catrice, O.; Ravaux, J.; Brown, S.C.; Higuet, D. Survey of genome size in 28 hydrothermal vent species covering 10 families. Genome 2009, 52, 524–536. [Google Scholar] [CrossRef]
  28. Zeng, X.; Zhang, Y.; Meng, L.; Fan, G.; Bai, J.; Chen, J.; Song, Y.; Seim, I.; Wang, C.; Shao, Z.; et al. Genome sequencing of deep-sea hydrothermal vent snails reveals adaptions to extreme environments. Gigascience 2020, 9, giaa139. [Google Scholar] [CrossRef]
  29. Biemont, C. Genome size evolution: Within-species variation in genome size. Heredity 2008, 101, 297–298. [Google Scholar] [CrossRef]
  30. Kazazian, H.H., Jr. Mobile elements: Drivers of genome evolution. Science 2004, 303, 1626–1632. [Google Scholar] [CrossRef]
  31. Hoen, D.R.; Bureau, T.E. Discovery of novel genes derived from transposable elements using integrative genomic analysis. Mol. Biol. Evol. 2015, 32, 1487–1506. [Google Scholar] [CrossRef]
  32. Janousek, V.; Laukaitis, C.M.; Yanchukov, A.; Karn, R.C. The role of retrotransposons in gene family expansions in the human and mouse genomes. Genome Biol. Evol. 2016, 8, 2632–2650. [Google Scholar] [CrossRef]
  33. Liu, R.Y.; Wang, K.; Liu, J.; Xu, W.J.; Zhou, Y.; Zhu, C.L.; Wu, B.S.; Li, Y.X.; Wang, W.; He, S.P.; et al. De Novo Genome Assembly of Limpet (Gastropoda: Pectinodontidae): The First Reference Genome of a Deep-Sea Gastropod Endemic to Cold Seeps. Genome Biol. Evol. 2020, 12, 905–910. [Google Scholar] [CrossRef]
  34. Patra, A.K.; Ho, P.-T.; Jun, S.; Lee, S.J.; Kim, Y.; Won, Y.J. Genome assembly of the Korean intertidal mud-creeper Batillaria attramentaria. Sci. Data 2023, 10, 498. [Google Scholar] [CrossRef] [PubMed]
  35. Cai, H.; Li, Q.; Fang, X.; Li, J.; Curtis, N.E.; Altenburger, A.; Shibata, T.; Feng, M.; Maeda, T.; Schwartz, J.A.; et al. A draft genome assembly of the solar-powered sea slug Elysia chlorotica. Sci. Data 2019, 6, 190022. [Google Scholar] [CrossRef]
  36. Peng, C.; Niu, L.; Deng, J.; Yu, J.; Zhang, X.; Zhou, C.; Xing, J.; Li, J. Can-SINE dynamics in the giant panda and three other Caniformia genomes. Mob. DNA 2018, 9, 32. [Google Scholar] [CrossRef] [PubMed]
  37. Casacuberta, E.; Gonzalez, J. The impact of transposable elements in environmental adaptation. Mol. Ecol. 2013, 22, 1503–1517. [Google Scholar] [CrossRef] [PubMed]
  38. Van’t Hof, A.E.; Campagne, P.; Rigden, D.J.; Yung, C.J.; Lingley, J.; Quail, M.A.; Hall, N.; Darby, A.C.; Saccheri, I.J. The industrial melanism mutation in British peppered moths is a transposable element. Nature 2016, 534, 102–105. [Google Scholar] [CrossRef]
  39. Todd, R.T.; Wikoff, T.D.; Forche, A.; Selmecki, A. Genome plasticity in Candida albicans is driven by long repeat sequences. eLife 2019, 8, e45954. [Google Scholar] [CrossRef]
  40. Malik, H.S.; González, J.; Karasov, T.L.; Messer, P.W.; Petrov, D.A. Genome-wide patterns of adaptation to temperate environments associated with transposable elements in Drosophila. PLoS Genet. 2010, 6, e1000905. [Google Scholar] [CrossRef]
  41. Magwire, M.M.; Bayer, F.; Webster, C.L.; Cao, C.; Jiggins, F.M. Successive increases in the resistance of Drosophila to viral infection through a transposon insertion followed by a duplication. PLoS Genet. 2011, 7, e1002337. [Google Scholar] [CrossRef]
  42. Zhang, Y.; Cheng, J.; Sha, Z.; Hui, M. Population genetic structure and implication for adaptive differentiation of the snail (Gastropoda, Provannidae) in deep-sea chemosynthetic ecosystems. Zool. Scr. 2023, in press. [Google Scholar] [CrossRef]
  43. Ashley, M.V.; Dow, B.D. The use of microsatellite analysis in population biology: Background, methods and potential applications. Mol. Ecol. Evol. Approaches Appl. 1994, 69, 185–201. [Google Scholar] [CrossRef]
  44. Chakraborty, R.; Kimmel, M.; Stivers, D.N.; Davison, L.J.; Deka, R. Relative mutation rates at di-, tri-, and tetranucleotide microsatellite loci. Proc. Natl. Acad. Sci. USA 1997, 94, 1041–1046. [Google Scholar] [CrossRef] [PubMed]
  45. Xu, T.; Feng, D.; Tao, J.; Qiu, J.W. A new species of deep-sea mussel (Bivalvia: Mytilidae: Gigantidas) from the South China Sea: Morphology, phylogenetic position, and gill-associated microbes. Deep-Sea Res. PT. I 2019, 146, 79–90. [Google Scholar] [CrossRef]
  46. Zhang, K.; Sun, J.; Xu, T.; Qiu, J.W.; Qian, P.Y. Phylogenetic relationships and adaptation in deep-sea mussels: Insights from mitochondrial genomes. Int. J. Mol. Sci. 2021, 22, 1900. [Google Scholar] [CrossRef] [PubMed]
  47. Tomasco, I.H.; Lessa, E.P. The evolution of mitochondrial genomes in subterranean caviomorph rodents: Adaptation against a background of purifying selection. Mol. Phylogenet. Evol. 2011, 61, 64–70. [Google Scholar] [CrossRef]
  48. da Fonseca, R.R.; Johnson, W.E.; O’Brien, S.J.; Ramos, M.J.; Antunes, A. The adaptive evolution of the mammalian mitochondrial genome. BMC Genom. 2008, 9, 119. [Google Scholar] [CrossRef]
  49. Yu, L.; Wang, X.; Ting, N.; Zhang, Y. Mitogenomic analysis of Chinese snub-nosed monkeys: Evidence of positive selection in NADH dehydrogenase genes in high-altitude adaptation. Mitochondrion 2011, 11, 497–503. [Google Scholar] [CrossRef]
  50. Silva, G.; Lima, F.P.; Martel, P.; Castilho, R. Thermal adaptation and clinal mitochondrial DNA variation of European anchovy. Proc. R. Soc. B 2014, 281, 20141093. [Google Scholar] [CrossRef]
  51. Yang, M.; Gong, L.; Sui, J.; Li, X. The complete mitochondrial genome of Calyptogena marissinica (Heterodonta: Veneroida: Vesicomyidae): Insight into the deep-sea adaptive evolution of vesicomyids. PLoS ONE 2019, 14, e0217952. [Google Scholar] [CrossRef]
  52. Ning, T.; Xiao, H.; Li, J.; Hua, S.; Zhang, Y.P. Adaptive evolution of the mitochondrial ND6 gene in the domestic horse. Genet. Mol. Res. 2010, 9, 144–150. [Google Scholar] [CrossRef]
  53. Cheng, J.; Hui, M.; Li, Y.; Sha, Z. Genomic evidence of population genetic differentiation in deep-sea squat lobster Shinkaia crosnieri (crustacea: Decapoda: Anomura) from Northwestern Pacific hydrothermal vent and cold seep. Deep-Sea Res. PT. I 2020, 156, 103188. [Google Scholar] [CrossRef]
  54. Xu, T.; Sun, J.; Watanabe, H.K.; Chen, C.; Nakamura, M.; Ji, R.; Feng, D.; Lv, J.; Wang, S.; Bao, Z.; et al. Population genetic structure of the deep-sea mussel Bathymodiolus platifrons (Bivalvia: Mytilidae) in the Northwest Pacific. Evol. Appl. 2018, 11, 1915–1930. [Google Scholar] [CrossRef] [PubMed]
  55. Zhang, B.; Wu, Y.; Wang, X.; Jiang, W.; Yin, J.; Lin, Q. Comparative analysis of mitochondrial genome of a deep-sea crab Chaceon granulates reveals positive selection and novel genetic features. J. Oceanol. Limnol. 2019, 38, 427–437. [Google Scholar] [CrossRef]
  56. Sun, S.; Hui, M.; Wang, M.; Sha, Z. The complete mitochondrial genome of the alvinocaridid shrimp Shinkaicaris leurokolos (Decapoda, Caridea): Insight into the mitochondrial genetic basis of deep-sea hydrothermal vent adaptation in the shrimp. Comp. Biochem. Phys. D 2018, 25, 42–52. [Google Scholar] [CrossRef]
  57. Luo, Y.; Gao, W.; Gao, Y.; Tang, S.; Huang, Q.; Tan, X.; Chen, J.; Huang, T. Mitochondrial genome analysis of Ochotona curzoniae and implication of cytochrome c oxidase in hypoxic adaptation. Mitochondrion 2008, 8, 352–357. [Google Scholar] [CrossRef] [PubMed]
Animals 13 03313 g001
Figure 1. Sampling site and genome characteristics of P. glabra. (a) Map showing the sampling area of P. glabra. It is drawn by Ocean Data View (ODV) v.5.0. (b) K-mer (K = 17) analysis for the genome. (c) Correlation between GC content and sequencing depth of the assembled draft genome. The horizontal axis represents GC content, and the vertical axis represents sequencing depth. The red portion represents the denser area of points in this scatterplot. The rightmost plot shows the distribution of sequencing depth of contigs, with the horizontal coordinate representing the number of contigs. The topmost plot displays the distribution of GC content, with its vertical coordinate indicating the number of contigs. (d) Divergence rates of four TE types in P. glabra draft genome.
Figure 1. Sampling site and genome characteristics of P. glabra. (a) Map showing the sampling area of P. glabra. It is drawn by Ocean Data View (ODV) v.5.0. (b) K-mer (K = 17) analysis for the genome. (c) Correlation between GC content and sequencing depth of the assembled draft genome. The horizontal axis represents GC content, and the vertical axis represents sequencing depth. The red portion represents the denser area of points in this scatterplot. The rightmost plot shows the distribution of sequencing depth of contigs, with the horizontal coordinate representing the number of contigs. The topmost plot displays the distribution of GC content, with its vertical coordinate indicating the number of contigs. (d) Divergence rates of four TE types in P. glabra draft genome.
Animals 13 03313 g001
Animals 13 03313 g002
Figure 2. Statistics on microsatellite motifs in P. glabra draft genome. (a) Distribution of different microsatellite motif types. (b) Frequency of different dinucleotide and trinucleotide microsatellite motifs.
Figure 2. Statistics on microsatellite motifs in P. glabra draft genome. (a) Distribution of different microsatellite motif types. (b) Frequency of different dinucleotide and trinucleotide microsatellite motifs.
Animals 13 03313 g002
Animals 13 03313 g003
Figure 3. Mitochondrial genome map of P. glabra. Purple, pink, and green represent PCGs, tRNA genes, and rRNA, respectively. The control region is marked in yellow.
Figure 3. Mitochondrial genome map of P. glabra. Purple, pink, and green represent PCGs, tRNA genes, and rRNA, respectively. The control region is marked in yellow.
Animals 13 03313 g003
Animals 13 03313 g004
Figure 4. Phylogenetic tree and evolutionary rates of gastropod mitochondrial genes. (a) Phylogenetic tree of gastropods with Amphioctopus marginatus as outgroup. Bootstrap value is displayed on the branch. Red branches represent deep-sea gastropods. (b) Evolutionary rate comparison of mitochondrial genes between deep-sea lineage (Abyssochrysoidea) and shallow-water lineage (Tonnoidea, Conoidea, and Muricoidea). ω0 represents the dN/dS values of the shallow-water lineage, and ω1 represents the dN/dS values of the deep-sea lineage. ** indicates extremely significant difference with 0.001 < p-value < 0.01.
Figure 4. Phylogenetic tree and evolutionary rates of gastropod mitochondrial genes. (a) Phylogenetic tree of gastropods with Amphioctopus marginatus as outgroup. Bootstrap value is displayed on the branch. Red branches represent deep-sea gastropods. (b) Evolutionary rate comparison of mitochondrial genes between deep-sea lineage (Abyssochrysoidea) and shallow-water lineage (Tonnoidea, Conoidea, and Muricoidea). ω0 represents the dN/dS values of the shallow-water lineage, and ω1 represents the dN/dS values of the deep-sea lineage. ** indicates extremely significant difference with 0.001 < p-value < 0.01.
Animals 13 03313 g004
Table 1. Statistics on the genome survey sequencing data and assembled draft genome of P. glabra.
Table 1. Statistics on the genome survey sequencing data and assembled draft genome of P. glabra.
Sequencing Data
Raw bases (Gb)63.54
Q30 of raw data (%)88.50
GC content of raw data (%)45.13
Clean bases (Gb)55.70
Q30 of clean data (%)92.98
GC content of clean data (%)44.48
Genome Assembly
Number of contigs4,132,680
Contig N50 (bp)437
Number of scaffolds3,534,913
Scaffold N50 (bp)581
GC content of the assembly (%)45.52
Table 2. Statistics on transposal elements (TEs) in the assembled draft genome of P. glabra.
Table 2. Statistics on transposal elements (TEs) in the assembled draft genome of P. glabra.
RepBaseTE ProteinsDe NovoCombined TEs
Length (bp)Percentage in Genome Length (bp)Percentage in Genome Length (bp)Percentage in Genome Length (bp)Percentage in Genome
DNA91,183,0576.88%4,798,3830.36%35,706,3232.70%124,415,6169.39%
LINE31,192,7612.35%30,457,3522.30%47,851,7763.61%81,713,3066.17%
SINE7,573,6300.57%--57,325,0684.33%59,987,6774.53%
LTR27,154,2352.05%3,528,7670.27%21,823,8491.65%49,670,3103.75%
Other33,747-1401---35,148-
Unknown3,016,3830.23%15,420-243,550,40618.38%246,409,80318.60%
Total139,568,99310.53%38,782,1002.93%401,595,86830.31%532,071,28940.17%
Table 3. Information of putative SNPs within the mitochondrial genome of P. glabra.
Table 3. Information of putative SNPs within the mitochondrial genome of P. glabra.
Gene NameGene Length (bp)Mutation Number (Percentage)Amino Acid ChangeVariation Site (DNA)Mutation Type
cox1153633 (2.15%)---
cox268720 (2.91%)---
cox378029 (3.72%)A→G527Transversion
T→A592Transition
nad194219 (2.02%)---
nad2106232 (3.01%)S→N242Transition
243
V→I502
A→I886
887
M→V961
nad335413 (3.67%)I→T263Transition
S→F281
T→M284
nad4136243 (3.16%)D→N544Transition
nad4l2587 (2.71%)S→T280Transversion
nad5172262 (3.60%)I→T512Transition
Y→C1196
I→V1402
D→N1432
V→I1699
nad650116 (3.19%)S→P244Transition
cob114042 (3.68%)V→A704Transition
atp669612 (1.72%)---
atp81593 (1.89%)---
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Hui, M.; Zhang, Y.; Wang, A.; Sha, Z. The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents. Animals 2023, 13, 3313. https://doi.org/10.3390/ani13213313

AMA Style

Hui M, Zhang Y, Wang A, Sha Z. The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents. Animals. 2023; 13(21):3313. https://doi.org/10.3390/ani13213313

Chicago/Turabian Style

Hui, Min, Yu Zhang, Aiyang Wang, and Zhongli Sha. 2023. "The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents" Animals 13, no. 21: 3313. https://doi.org/10.3390/ani13213313

APA Style

Hui, M., Zhang, Y., Wang, A., & Sha, Z. (2023). The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents. Animals, 13(21), 3313. https://doi.org/10.3390/ani13213313

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop