Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Protein and Cells
2.2. Animals
2.3. Animal Experiments
2.4. Mice Splenic Lymphocytes Isolation
2.5. IFN-γ ELISpot Assays
2.6. CD4+/CD8+ T Cell Subclass Ratio
2.7. Indirect ELISA
2.8. Cytokine Assays
2.9. Prediction of T Cell Epitopes of ASFV F317L Protein
2.10. F317L Protein T Cell Epitope Screening
2.11. Statistical Analysis
3. Results
3.1. The F317L Protein Stimulates Splenic Lymphocytes to Secrete IFN-γ
3.2. The F317L Protein Can Induce an Increase in the CD4+/CD8+ Ratio of Mouse Splenic Lymphocytes and Promote Differentiation to CD4+ T Cell Subsets
3.3. The F317L Protein Induced and the Secretion of High Specific Antibodies in Humoral Immunity in Immunized Mice
3.4. F317L Protein Promoted the Secretion of Immune-Related Cytokines (IL-2, IL-4, IFN-γ, and TNF-α) in Immunized Mice
3.5. Peptide(246−255aa) Is Potential T Cell Active Epitopes of F317L Protein
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number | Peptide | Number | Peptide | Number | Peptide |
---|---|---|---|---|---|
F1 | 1MVETQMDKL9 | F11 | 115DDDREWCGR119 | F21 | 178CGGMPSICD186 |
F2 | 26SNAHITQTM34 | F12 | 117CGRINMING125 | F22 | 204IISSNQVGM212 |
F3 | 40ENHSVDGGA48 | F13 | 123INGVPEIVE131 | F23 | 211GMLTVDKRII220 |
F4 | 46GGAAKNVSK54 | F14 | 129IVEIIPSPY137 | F24 | 219IIVDLWANEN228 |
F5 | 53SKGKSSPKE61 | F15 | 137YRAGENIYF145 | F25 | 246SRRSLVNPWT255 |
F6 | 57SSPKEKKHW65 | F16 | 148EAMMPADIY156 | F26 | 262ILQDYGIEY270 |
F7 | 60KEKKHWTEF68 | F17 | 157SRVANKPAMF166 | F27 | 270YIIFPSNDF278 |
F8 | 63KHWTEFESW71 | F18 | 160ANKPAMFVF168 | F28 | 276NDFFIYEDE284 |
F9 | 73QLSKSKRSF81 | F19 | 166FVFHTHPNL174 | F29 | 294TNFFTLHEL302 |
F10 | 76KSKRSFKEY84 | F20 | 172PNLGSCCGG180 |
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Huang, Y.; Zhai, W.; Wang, Z.; He, Y.; Tao, C.; Chu, Y.; Pang, Z.; Zhu, H.; Jia, H. Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes. Animals 2024, 14, 1331. https://doi.org/10.3390/ani14091331
Huang Y, Zhai W, Wang Z, He Y, Tao C, Chu Y, Pang Z, Zhu H, Jia H. Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes. Animals. 2024; 14(9):1331. https://doi.org/10.3390/ani14091331
Chicago/Turabian StyleHuang, Ying, Wenzhu Zhai, Zhen Wang, Yuheng He, Chunhao Tao, Yuanyuan Chu, Zhongbao Pang, Hongfei Zhu, and Hong Jia. 2024. "Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes" Animals 14, no. 9: 1331. https://doi.org/10.3390/ani14091331