Effect of Anacardic Acid against Thiram Induced Tibial Dyschondroplasia in Chickens via Regulation of Wnt4 Expression
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Chickens Husbandry
2.3. Morphological, Production Parameters Analysis and Sample Collection
2.4. Hematoxylin and Eosin (H and E) Staining
2.5. Measurement of SOD, MDA Contents, GSH-Px, and T-AOC Activity
2.6. Reverse Transcription Quantitative-Polymerase Chain Reaction (RT-qPCR)
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Effects of AA on Lameness, Growth-Plate and Morphology
3.2. TD Score
3.3. Tibial Parameters Evaluation
3.4. Histological Examination of Tibial Growth Plate
3.5. Liver Antioxidant Levels
3.6. Effects of AA on Expression of Wnt4 Gene in Growth Plate
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jiang, X.; Zhang, H.; Mehmood, K.; Li, K.; Zhang, L.; Yao, W.; Tong, X.; Li, A.; Wang, Y.; Jiang, J.; et al. Effect of Anacardic Acid against Thiram Induced Tibial Dyschondroplasia in Chickens via Regulation of Wnt4 Expression. Animals 2019, 9, 82. https://doi.org/10.3390/ani9030082
Jiang X, Zhang H, Mehmood K, Li K, Zhang L, Yao W, Tong X, Li A, Wang Y, Jiang J, et al. Effect of Anacardic Acid against Thiram Induced Tibial Dyschondroplasia in Chickens via Regulation of Wnt4 Expression. Animals. 2019; 9(3):82. https://doi.org/10.3390/ani9030082
Chicago/Turabian StyleJiang, Xiong, Hui Zhang, Khalid Mehmood, Kun Li, Lihong Zhang, Wangyuan Yao, Xiaole Tong, Aoyun Li, Yaping Wang, Jinhuan Jiang, and et al. 2019. "Effect of Anacardic Acid against Thiram Induced Tibial Dyschondroplasia in Chickens via Regulation of Wnt4 Expression" Animals 9, no. 3: 82. https://doi.org/10.3390/ani9030082