MicroRNA Sequencing Reveals the Effect of Different Levels of Non-Fibrous Carbohydrate/Neutral Detergent Fiber on Rumen Development in Calves
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.1.1. Ethics Statement
2.1.2. Experimental Animals and RNA Isolation
2.1.3. Preparation and Observation of Rumen Sections
2.2. MicroRNA Sequencing
2.2.1. miRNA library Construction and Illumina Deep Sequencing
2.2.2. Sequence Analyses
2.2.3. Differential Expression Analysis of miRNAs
2.2.4. MiRNA Target Prediction, Functional Annotation, and Interaction Networks
2.3. Verification of Sequencing Results
2.3.1. Validation of Relative Expression of miRNAs and mRNAs
2.3.2. Vector Construction
2.3.3. Cell Culture and Luciferase Reporter Assay
2.4. Statistical Analyses
3. Results and Discussion
3.1. Effect of NFC/NDF Levels on Rumen Development of Calf
3.2. Overview of Small RNA Deep Sequencing Data
3.3. Identification of Differentially Expressed miRNAs
3.4. Prediction of DEmiRNA Target Genes
3.5. Functional Enrichment Analysis
3.6. Targeting Effect of Bta-miR-128 on PPARG and SLC16A1
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Xue, M.; Wang, K.; Wang, A.; Li, R.; Wang, Y.; Sun, S.; Yan, D.; Song, G.; Xu, H.; Sun, G.; et al. MicroRNA Sequencing Reveals the Effect of Different Levels of Non-Fibrous Carbohydrate/Neutral Detergent Fiber on Rumen Development in Calves. Animals 2019, 9, 496. https://doi.org/10.3390/ani9080496
Xue M, Wang K, Wang A, Li R, Wang Y, Sun S, Yan D, Song G, Xu H, Sun G, et al. MicroRNA Sequencing Reveals the Effect of Different Levels of Non-Fibrous Carbohydrate/Neutral Detergent Fiber on Rumen Development in Calves. Animals. 2019; 9(8):496. https://doi.org/10.3390/ani9080496
Chicago/Turabian StyleXue, Mingming, Kejun Wang, Ansi Wang, Ruiting Li, Yadong Wang, Shuaijie Sun, Duo Yan, Guohua Song, Huifen Xu, Guirong Sun, and et al. 2019. "MicroRNA Sequencing Reveals the Effect of Different Levels of Non-Fibrous Carbohydrate/Neutral Detergent Fiber on Rumen Development in Calves" Animals 9, no. 8: 496. https://doi.org/10.3390/ani9080496