Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Generation of hPSC-Derived Astrocytes
2.2. Immunocytochemistry
2.3. RNA Sequencing and Analysis
2.4. RNA Expression Analysis with Quantitative Real Time PCR (qRT-PCR)
2.5. Calcium Imaging
2.6. Cytokine Array
3. Results
3.1. Patterning and Differentiation of Neural Progenitors
3.2. Astrocyte Specification
3.3. Properties of hPSC-Derived Astrocytes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peteri, U.-K.; Pitkonen, J.; Utami, K.H.; Paavola, J.; Roybon, L.; Pouladi, M.A.; Castrén, M.L. Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity. Brain Sci. 2021, 11, 209. https://doi.org/10.3390/brainsci11020209
Peteri U-K, Pitkonen J, Utami KH, Paavola J, Roybon L, Pouladi MA, Castrén ML. Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity. Brain Sciences. 2021; 11(2):209. https://doi.org/10.3390/brainsci11020209
Chicago/Turabian StylePeteri, Ulla-Kaisa, Juho Pitkonen, Kagistia Hana Utami, Jere Paavola, Laurent Roybon, Mahmoud A. Pouladi, and Maija L. Castrén. 2021. "Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity" Brain Sciences 11, no. 2: 209. https://doi.org/10.3390/brainsci11020209