Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Care
2.2. Anesthesia/Surgery
2.3. LIPUS on Hippocampus
2.4. Behavioral Test
2.5. Real Time-qPCR
2.6. Immunofluorescent Staining
2.7. Hematoxylin-Eosin (HE) Staining
2.8. Western Blotting
2.9. Statistical Analysis
3. Results
3.1. LIPUS Could Safely Induce Neuronal Activation in Dorsal Hippocampus
3.2. LIPUS Attenuates Hippocampus-Dependent Spatial Reference Learning and Memory Injury after A/S in Aged Mice
3.3. LIPUS Reduces the Neuroinflammation of Hippocampus after A/S in Aged Mice
3.4. LIPUS Reverses the Reduced Synapse-Related Proteins of the Hippocampus after A/S in Aged Mice
3.5. LIPUS Suppresses the Overexpression of Piezo1 after A/S in Aged Mice
3.6. LIPUS May Improve Synaptic Function through Modulating Piezo1/Calpain/Erk Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Protein Name | Forward | Reverse |
---|---|---|---|
Tnf | TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
Il1b | IL-1β | CACTACAGGCTCCGAGATGAACAAC | TGTCGTTGCTTGGTTCTCCTTGTAC |
Il6 | IL-6 | CTCCCAACAGACCTGTCTATAC | CCATTGCACAACTCTTTTCTCA |
Piezo1 | Piezo1 | GAATGTGATTGGGCAGCGTATGAAC | GAACAGCGTGAGGAACAGACAGTAG |
Actb | β-actin | TATGCTCTCCCTCACGCCATCC | GTCACGCACGATTTCCCTCTCAG |
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Wang, Q.; Liu, T.; Chang, H.; Li, Z.; Chen, L.; Mi, X.; Xing, H.; Wang, X.; Hong, J.; Liu, K.; et al. Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice. Brain Sci. 2023, 13, 657. https://doi.org/10.3390/brainsci13040657
Wang Q, Liu T, Chang H, Li Z, Chen L, Mi X, Xing H, Wang X, Hong J, Liu K, et al. Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice. Brain Sciences. 2023; 13(4):657. https://doi.org/10.3390/brainsci13040657
Chicago/Turabian StyleWang, Qian, Taotao Liu, Huixian Chang, Zhengqian Li, Lei Chen, Xinning Mi, Huayi Xing, Xiaoxiao Wang, Jingshu Hong, Kaixi Liu, and et al. 2023. "Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice" Brain Sciences 13, no. 4: 657. https://doi.org/10.3390/brainsci13040657