Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Drugs and Reagents
2.2. S100β Injections
2.3. Electrophysiology
2.4. Immunohistochemistry
2.5. Confocal Microscopy and Morphometric Analysis
2.6. Sholl Analysis of Bergmann Glia Cells
2.7. Statistical and Mathematical Analysis
3. Results
3.1. Exogenous S100β Alters Morphology of Bergmann Glia
3.2. Extracellular S100β Alters Morphology of Purkinje Cells
3.3. Extracellular S100β Alters Synaptic Transmission in PFs and PCs
3.4. Similarities in Changes in Synaptic Transmission in S100β-Injected Mice and Ataxin1 Mutant Animals
3.5. Extracellular S100β Alters Endocannabinoid-Dependent Short Term Plasticity in PF–PC Synapses
3.6. Effects of FC on PF–PC Transmission and Endocannabinoid Short Term Plasticity in PF–PC Synapses
3.7. Slowdown of Glutamate Uptake in Bergmann Glia by TBOA Leads to Alteration of PF-EPSC Kinetic, But Does Not Change Endocannabinoid Short Term Plasticity in PF-PC Synapses
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Belozor, O.S.; Yakovleva, D.A.; Potapenko, I.V.; Shuvaev, A.N.; Smolnikova, M.V.; Vasilev, A.; Pozhilenkova, E.A.; Shuvaev, A.N. Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells. Brain Sci. 2019, 9, 80. https://doi.org/10.3390/brainsci9040080
Belozor OS, Yakovleva DA, Potapenko IV, Shuvaev AN, Smolnikova MV, Vasilev A, Pozhilenkova EA, Shuvaev AN. Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells. Brain Sciences. 2019; 9(4):80. https://doi.org/10.3390/brainsci9040080
Chicago/Turabian StyleBelozor, Olga S., Dariya A. Yakovleva, Ilya V. Potapenko, Andrey N. Shuvaev, Marina V. Smolnikova, Alex Vasilev, Elena A. Pozhilenkova, and Anton N. Shuvaev. 2019. "Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells" Brain Sciences 9, no. 4: 80. https://doi.org/10.3390/brainsci9040080