A Reductive Metabolic Switch Protects Infants with Transposition of Great Arteries Undergoing Atrial Septostomy against Oxidative Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Analytical Procedures
2.2.1. Lipid Peroxidation Biomarkers
2.2.2. Untargeted Ultra-Performance Liquid Chromatography Coupled to Time-of-Flight Mass Spectrometry (UPLC-TOFMS) Metabolomic Analysis
2.2.3. Data Processing and Statistical Analysis
3. Results
3.1. Clinical Results
3.2. Lipid Peroxidation Biomarkers
3.3. Effect of Time on the Plasma Metabolome
3.4. Correlation of Metabolic Features with FTOE
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Analyte [p.d.u.] | RT [min] | Parent Ion (m/z) | Daughter Ion (m/z) | CE (eV) | Cone Voltage (V) |
---|---|---|---|---|---|
IsoPs | 4.3–6.6 | 353.20 | 115.00 | 30 | 35 |
Di-homo-IsoPs | 5.0–6.8 | 381.00 | 143.00 | 20 | 20 |
Di-homo-IsoFs | 3.5–6.5 | 397.00 | 155.00 | 24 | 35 |
NeuroFs | 2.70–6.50 | 393.00 | 193.00 | 20 | 35 |
IsoFs | 2.1–6.60 | 369.20 | 115.00 | 20 | 45 |
NeuroPs | 2.30–6.50 | 377.00 | 101.00 | 20 | 35 |
Patients’ Demographics | All Patients | BAS Patients |
---|---|---|
Patients recruited, N (%) | 12 (100) | 9 (100) |
Gender, N (%) | ||
- Male | 10 (83) | 8 (89) |
- Female | 2 (17) | 1 (11) |
Balloon atrial septostomy, N (%) | 9 (75) | 9 (100) |
Expired, N (%) | 1 (8.3) | 0 (0) |
Prenatal diagnosis, N (%) | 6 (50) | 4 (44) |
Gestational age, weeks (median, range) | 39.5 (37.0–41.3) | 39.9 (38.4–41.3) |
Birth weight, kg (median, range) | 3.4 (2.0–4.0) | 3.5 (3.1–4.0) |
Postnatal age, hours (median, range) | ||
- At the time of the first measured peripheral saturation | 1.7 (0.5–13.2) | 2.3 (0.5–6.7) |
- At time of BAS | 3.2 (2.0–9.1) | |
Saturation, % (median, range) | ||
- First peripheral saturation (SpO2) | 79 (51–91) | 77 (56–88) |
- Lowest peripheral saturation (SpO2) | 65 (39–86) | 65 (39–86) |
- First regional cerebral oxygen saturation (rcSO2) | 58 (37–79) | 53 (37–79) |
- 5-min post septostomy peripheral saturation (SpO2) | 86 (62–91) | 86 (62–91) |
Blood lactate, mmol/L (median, range) | ||
- Before BAS | 3.2 (1.3–4.9) | 3.6 (1.3–4.9) |
- 5-min after BAS | 2.7 (1.4–5.0) | 3.3 (1.4–5.0) |
Pathway Name | Compound Code (KEGG) | Pathway ID | # Hits | # Sig Hits | p-Value |
---|---|---|---|---|---|
Pentose phosphate pathway | C01801; C00672; C00121; C00121; C00257; C00257; C00258 | map00030 | 7 | 7 | 0.00002 |
Pentose and glucuronate interconversions | C01068; C00181; C00259; C00310; C00312; C00379; C00532; C00379; C00532; C00379; C00379; C00532; C00257; C00257; C00029; C00052; C00191; C00618; C02266 | map00040 | 11 | 7 | 0.003 |
Ascorbate and aldarate metabolism | C00137; C00029; C02670; C00191; C00800 | map00053 | 8 | 5 | 0.013 |
Inositol phosphate metabolism | C00137; C00222; C00191 | map00562 | 4 | 3 | 0.03 |
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Piñeiro-Ramos, J.D.; Rahkonen, O.; Korpioja, V.; Quintás, G.; Pihkala, J.; Pitkänen-Argillander, O.; Rautiainen, P.; Andersson, S.; Kuligowski, J.; Vento, M. A Reductive Metabolic Switch Protects Infants with Transposition of Great Arteries Undergoing Atrial Septostomy against Oxidative Stress. Antioxidants 2021, 10, 1502. https://doi.org/10.3390/antiox10101502
Piñeiro-Ramos JD, Rahkonen O, Korpioja V, Quintás G, Pihkala J, Pitkänen-Argillander O, Rautiainen P, Andersson S, Kuligowski J, Vento M. A Reductive Metabolic Switch Protects Infants with Transposition of Great Arteries Undergoing Atrial Septostomy against Oxidative Stress. Antioxidants. 2021; 10(10):1502. https://doi.org/10.3390/antiox10101502
Chicago/Turabian StylePiñeiro-Ramos, José David, Otto Rahkonen, Virpi Korpioja, Guillermo Quintás, Jaana Pihkala, Olli Pitkänen-Argillander, Paula Rautiainen, Sture Andersson, Julia Kuligowski, and Máximo Vento. 2021. "A Reductive Metabolic Switch Protects Infants with Transposition of Great Arteries Undergoing Atrial Septostomy against Oxidative Stress" Antioxidants 10, no. 10: 1502. https://doi.org/10.3390/antiox10101502