Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Chemicals and Reagents
2.3. Preparation of Sample and Standard Solution
2.4. HPLC Condition
2.5. Experimental Animals and Protocol
2.6. Bronchoalveolar Lavage Fluid (BALF) and Serum Analysis
2.7. Histological Examination of Lung Tissue
2.8. Western Blot
2.9. Gelatin Zymography
2.10. Statistical Analysis
3. Results
3.1. HPLC Analysis of CRE
3.2. CRE Inhibited Eosinophilia and Airway Hyperresponsiveness (AHR) in Animals with Asthma
3.3. CRE Decreased Inflammatory Cytokines and OVA-Specific IgE in Animals with Asthma
3.4. CRE Reduced Airway Inflammation and Mucus Secretion of Lung Tissue from Animals with Asthma
3.5. CRE Elevated the Expression of the Nrf2/HO-1/NQO1 in Animals with Asthma
3.6. CRE Suppressed NF-κB Phosphorylation and MMP-9 Expression in Animals with Asthma
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Compound | Contents (Mean ± SD, n = 3) | |
---|---|---|
mg/g | % | |
Caffeic acid | 0.62 ± 0.01 | 0.06 |
Ferulic acid | 1.35 ± 0.04 | 0.14 |
Isoferulic acid | 8.09 ± 0.24 | 0.81 |
Cimicifugic acid B | 1.86 ± 0.03 | 0.19 |
Cimicifugic acid F | 10.93 ± 0.14 | 1.09 |
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Lim, J.-O.; Song, K.H.; Lee, I.S.; Lee, S.-J.; Kim, W.-I.; Pak, S.-W.; Shin, I.-S.; Kim, T. Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma. Antioxidants 2021, 10, 1626. https://doi.org/10.3390/antiox10101626
Lim J-O, Song KH, Lee IS, Lee S-J, Kim W-I, Pak S-W, Shin I-S, Kim T. Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma. Antioxidants. 2021; 10(10):1626. https://doi.org/10.3390/antiox10101626
Chicago/Turabian StyleLim, Je-Oh, Kwang Hoon Song, Ik Soo Lee, Se-Jin Lee, Woong-Il Kim, So-Won Pak, In-Sik Shin, and Taesoo Kim. 2021. "Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma" Antioxidants 10, no. 10: 1626. https://doi.org/10.3390/antiox10101626