Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bone Marrow Derived Monocyte Isolation
2.2. Bone Marrow Derived Macrophage (BMDM) Culture
2.3. Ascorbate Uptake Measurement
2.4. Immunostaining and Morphometric Analysis
2.5. Morphometric and Immunostaining Analysis
2.6. Flow Cytometry
2.7. Measuring BMDM Gene Expression
2.8. Measuring Proteins by Antibody Array and ELISA
2.9. Statistical Analysis
3. Results
3.1. Cell Surface Markers of Isolated Bone Marrow Cells in Culture with LLCM and Hypoxia
3.2. Ascorbate Uptake of LLCM-Grown Bone Marrow Derived Macrophages
3.3. Morphology of LLCM Grown BMDMs and with Hypoxia Stimulation
3.4. Gene Expression of LLCM Grown BMDMs and with Hypoxia Stimulation
3.5. Protein Secretion of LLCM Grown BMDMs and with Hypoxia Stimulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
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Ratio LLCM + Asc/LLCM | 21% O2 | 1% O2 |
---|---|---|
Top 5 increased | VE-cadherin (3.5), MMP-2 (2.1), LRP-6 (2.0), IL-1 R6 (2.0), Neurturin (1.7) | TRAIL (9.4), IGFBP-7 (3.3), TYRO3 (3.2), MMP-2 (2.8), TIMP-1 (2.3) |
Top 5 decreased | FGF R3 (−33), Granzyme D (−20), MCP-1 (−20), TLR4 (−14), IL-5 Rα (−13) | CCR4 (−6.7), IL-2 (−4.2), bFGF (−2.5), Decorin (−2.4), IL-6 (−2.3) |
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Ang, A.D.; Vissers, M.C.M.; Burgess, E.R.; Currie, M.J.; Dachs, G.U. Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions. Antioxidants 2021, 10, 430. https://doi.org/10.3390/antiox10030430
Ang AD, Vissers MCM, Burgess ER, Currie MJ, Dachs GU. Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions. Antioxidants. 2021; 10(3):430. https://doi.org/10.3390/antiox10030430
Chicago/Turabian StyleAng, Abel D., Margreet C. M. Vissers, Eleanor R. Burgess, Margaret J. Currie, and Gabi U. Dachs. 2021. "Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions" Antioxidants 10, no. 3: 430. https://doi.org/10.3390/antiox10030430