Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Tartary Buckwheat Extract
2.2. Antibodies
2.3. Cell Culture
2.4. MTT (Methylthialazole Tetrazolium) Assay for Cell Death
2.5. Western Blotting
2.6. Immunofluorescence and Confocal Microscope Observation
3. Results
3.1. TBE Induces Autophagy
3.2. TBE Induces Autophagosome Formation
3.3. TBE Enhances Dephosphorylation of mTORC1 Substrates
3.4. TBE Induces Autophagy against Protein Aggregation
3.5. Quercetin Is Responsible for the TBE Effect on Autophagy Induction
3.6. TBE and Quercetin Promote Aggrephagy in Liver Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ikari, S.; Yang, Q.; Lu, S.-L.; Liu, Y.; Hao, F.; Tong, G.; Lu, S.; Noda, T. Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations. Antioxidants 2021, 10, 1217. https://doi.org/10.3390/antiox10081217
Ikari S, Yang Q, Lu S-L, Liu Y, Hao F, Tong G, Lu S, Noda T. Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations. Antioxidants. 2021; 10(8):1217. https://doi.org/10.3390/antiox10081217
Chicago/Turabian StyleIkari, Sumiko, Qiang Yang, Shiou-Ling Lu, Yuancai Liu, Feike Hao, Guoqiang Tong, Shiguang Lu, and Takeshi Noda. 2021. "Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations" Antioxidants 10, no. 8: 1217. https://doi.org/10.3390/antiox10081217
APA StyleIkari, S., Yang, Q., Lu, S. -L., Liu, Y., Hao, F., Tong, G., Lu, S., & Noda, T. (2021). Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations. Antioxidants, 10(8), 1217. https://doi.org/10.3390/antiox10081217