Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and H2O2 Treatment
2.2. Cell Viability Assay
2.3. Analysis of the Morphological Characteristics of Cells
2.4. Capacity of Cells to Reseeding Post H2O2 Treatments
2.5. Senescence-Associated β-Galactosidase Assay
2.6. Determination of Population Doubling Rate
2.7. Mitochondrial Superoxide Anion Detection
2.8. Reactive Oxygen Species Detection
2.9. Indirect Inmmunofluorescence
2.10. Statistical Analysis
3. Results
3.1. Preliminary Screening of H2O2 Treated Cells: Cell Viability Analysis
3.2. Morphological Changes and Capacity of Cells to Reseeding Post H2O2 Treatments
3.3. H2O2 Increase SA β-Galactosidase Activity in Cells
3.4. Population Doubling Rate Post H2O2 Treatments
3.5. H2O2-Treatment Induced Mitochondrial ROS in Cells
3.6. H2O2 Increase Levels of Reactive Oxygen Species
3.7. H2O2 Treatment Increased Levels of 8-Oxoguanine in Cells
3.8. Foxo3 Increased in Cells Post H2O2 Treatments
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rivas-Chacón, L.d.M.; Martínez-Rodríguez, S.; Madrid-García, R.; Yanes-Díaz, J.; Riestra-Ayora, J.I.; Sanz-Fernández, R.; Sánchez-Rodríguez, C. Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss. Antioxidants 2021, 10, 1497. https://doi.org/10.3390/antiox10091497
Rivas-Chacón LdM, Martínez-Rodríguez S, Madrid-García R, Yanes-Díaz J, Riestra-Ayora JI, Sanz-Fernández R, Sánchez-Rodríguez C. Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss. Antioxidants. 2021; 10(9):1497. https://doi.org/10.3390/antiox10091497
Chicago/Turabian StyleRivas-Chacón, Luz del Mar, Sofía Martínez-Rodríguez, Raquel Madrid-García, Joaquín Yanes-Díaz, Juan Ignacio Riestra-Ayora, Ricardo Sanz-Fernández, and Carolina Sánchez-Rodríguez. 2021. "Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss" Antioxidants 10, no. 9: 1497. https://doi.org/10.3390/antiox10091497